##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
AseI AsnI AspEI BbeI BbrPI
BcgI BclI BfrI BsaAI BseAI
BsiWI BsmI BspDI BspEI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoRI EcoRV FbaI
HindIII HpaI KasI MfeI MluI
MroI MunI NarI NdeI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SalI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 557 GGTACC G^GTACC
AclI 4939 AACGTT AA^CGTT
AcsI 1324 AAATTT [GA]^AATT[CT]
AflIII 1346 ACATGT A^C[GA][CT]GT
ApoI 1324 AAATTT [GA]^AATT[CT]
AscI 2071 GGCGCGCC GG^CGCGCC
Asp718I 557 GGTACC G^GTACC
AspI 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 939 CCTAGG C^CTAGG
BciVI 1534 GTATCC GTATCC(6/5)
BglII 1409 AGATCT A^GATCT
BlnI 939 CCTAGG C^CTAGG
BsaWI 1702 TCCGGT [AT]^CCGG[AT]
BspLU11I 1346 ACATGT A^CATGT
DraIII 2153 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3103 ATGCAT ATGCA^T
HincII 4430 GTCAAC GT[CT]^[GA]AC
HindII 4430 GTCAAC GT[CT]^[GA]AC
KpnI 557 GGTACC GGTAC^C
NheI 4616 GCTAGC G^CTAGC
NotI 1148 GCGGCCGC GC^GGCCGC
NsiI 3103 ATGCAT ATGCA^T
PflFI 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2303 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4939 AACGTT AA^CGTT
SapI 3917 GCTCTTC GCTCTTC(1/4)
SbfI 927 CCTGCAGG CCTGCA^GG
SfiI 879 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 927 CCTGCAGG CCTGCA^GG
SspI 5068 AATATT AAT^ATT
StuI 4626 AGGCCT AGG^CCT
Tth111I 147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4297 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
Aor51HI 957 AGCGCT AGC^GCT
1394 AGCGCT
Asp700I 795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1359 GAACCAGTTC
AviII 431 TGCGCA TGC^GCA
3167 TGCGCA
BamHI 990 GGATCC G^GATCC
1559 GGATCC
BbvCI 72 CCTCAGC CCTCAGC(-5/-2)
3350 CCTCAGC
BsaBI 609 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4135 GATTTTTATC
BsmBI 1730 CGTCTC CGTCTC(1/5)
2830 GAGACG
BspHI 1355 TCATGA T^CATGA
3071 TCATGA
BsrDI 3039 CATTGC GCAATG(2/0)
3899 CATTGC
BssHII 622 GCGCGC G^CGCGC
2072 GCGCGC
BssSI 28 CACGAG CACGAG(-5/-1)
1122 CTCGTG
BstAPI 768 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1488 GCACTCACTGC
Bsu36I 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
Eco47III 957 AGCGCT AGC^GCT
1394 AGCGCT
Eco81I 1563 CCTGAGG CC^T[ACGT]AGG
2526 CCTGAGG
FspI 431 TGCGCA TGC^GCA
3167 TGCGCA
KspI 2261 CCGCGG CCGC^GG
2421 CCGCGG
MamI 609 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4135 GATTTTTATC
NcoI 650 CCATGG C^CATGG
1868 CCATGG
NspI 1346 ACATGT [GA]CATG^[CT]
1388 ACATGC
PaeR7I 715 CTCGAG C^TCGAG
1806 CTCGAG
RcaI 1355 TCATGA T^CATGA
3071 TCATGA
SacII 2261 CCGCGG CCGC^GG
2421 CCGCGG
XhoI 715 CTCGAG C^TCGAG
1806 CTCGAG
XmnI 795 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1359 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI FseI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI