##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BglI BglII
BsaBI BsaHI BsaWI BseAI BsiEI
BsmBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MluI MluNI MroI MscI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
TaqI TthHB8I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 275 CTTAAG C^TTAAG
Alw44I 3815 GTGCAC G^TGCAC
ApaI 2403 GGGCCC GGGCC^C
ApaLI 3815 GTGCAC G^TGCAC
Asp700I 3878 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2292 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1940 CCCGAG C^[CT]CG[GA]G
AvrII 3899 CCTAGG C^CTAGG
BcnI 1539 CCCGG CC^[GC]GG
BfrI 275 CTTAAG C^TTAAG
BlnI 3899 CCTAGG C^CTAGG
BsaAI 3179 CACGTA [CT]AC^GT[GA]
BsaI 2755 GGTCTC GGTCTC(1/5)
BseRI 847 CTCCTC GAGGAG(10/8)
BsiWI 3279 CGTACG C^GTACG
BsoBI 1940 CCCGAG C^[CT]CG[GA]G
BspMI 1875 GCAGGT ACCTGC(4/8)
BsrFI 1806 GCCGGC [GA]^CCGG[CT]
Cfr10I 1806 GCCGGC [GA]^CCGG[CT]
EcoRI 3671 GAATTC G^AATTC
HgaI 3175 GCGTC GACGC(5/10)
HpaI 1518 GTTAAC GTT^AAC
MfeI 2435 CAATTG C^AATTG
MunI 2435 CAATTG C^AATTG
NaeI 1806 GCCGGC GCC^GGC
NciI 1539 CCCGG CC^[GC]GG
NgoMIV 1806 GCCGGC G^CCGGC
PflFI 2292 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2060 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 70 GAGCTC GAGCT^C
ScaI 1443 AGTACT AGT^ACT
Tth111I 2292 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4500 TCTAGA T^CTAGA
XcmI 2309 CCAAGCCAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3878 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3419 GTCTAC GT^[AC][GT]AC
3439 GTCTAC
AccII 1973 CGCG CG^CG
4342 CGCG
AclI 1193 AACGTT AA^CGTT
3978 AACGTT
AflIII 912 ACATGT A^C[GA][CT]GT
2791 ACATGT
AlwI 1149 GATCC GGATC(4/5)
1497 GGATC
AlwNI 1741 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1930 CAGGCCCTG
AocI 39 CCTCAGG CC^T[ACGT]AGG
341 CCTAAGG
AseI 3695 ATTAAT AT^TAAT
4281 ATTAAT
AsnI 3695 ATTAAT AT^TAAT
4281 ATTAAT
BpmI 1829 CTCCAG CTGGAG(16/14)
1369 CTGGAG
BspLU11I 912 ACATGT A^CATGT
2791 ACATGT
BstUI 1973 CGCG CG^CG
4342 CGCG
BstYI 1148 AGATCC [GA]^GATC[CT]
1497 GGATCT
Bsu36I 39 CCTCAGG CC^T[ACGT]AGG
341 CCTAAGG
CfoI 1873 GCGC GCG^C
1974 GCGC
DraIII 696 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3360 CACAAAGTG
Eco81I 39 CCTCAGG CC^T[ACGT]AGG
341 CCTAAGG
EcoNI 284 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
298 CCTTGAAAAGG
FauI 1257 CCCGC CCCGC(4/6)
3036 CCCGC
HhaI 1873 GCGC GCG^C
1974 GCGC
HinP1I 1873 GCGC G^CGC
1974 GCGC
HincII 1074 GTCAAC GT[CT]^[GA]AC
1518 GTTAAC
HindII 1074 GTCAAC GT[CT]^[GA]AC
1518 GTTAAC
MflI 1148 AGATCC [GA]^GATC[CT]
1497 GGATCT
MvnI 1973 CGCG CG^CG
4342 CGCG
NcoI 860 CCATGG C^CATGG
2482 CCATGG
PshBI 3695 ATTAAT AT^TAAT
4281 ATTAAT
Psp1406I 1193 AACGTT AA^CGTT
3978 AACGTT
SspI 585 AATATT AAT^ATT
628 AATATT
XhoII 1148 AGATCC [GA]^GATC[CT]
1497 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BlpI BmyI
Bpu1102I BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I