##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
AspEI BclI BfrI BsaBI BsiWI
BsmBI BspDI BspHI BspLU11I Bst1107I
BstEII BstPI BstZ17I ClaI Eam1105I
EcoO65I FbaI FseI HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfiI
SnaBI SpeI Sse8387I SspI StuI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2594 GGTACC G^GTACC
AccIII 118 TCCGGA T^CCGGA
AcyI 29 GGCGCC G[GA]^CG[CT]C
Aor51HI 1130 AGCGCT AGC^GCT
Asp718I 2594 GGTACC G^GTACC
AspI 214 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 242 TGCGCA TGC^GCA
BamHI 1931 GGATCC G^GATCC
BbeI 29 GGCGCC GGCGC^C
BbrPI 2143 CACGTG CAC^GTG
BciVI 2686 GTATCC GTATCC(6/5)
BsaHI 29 GGCGCC G[GA]^CG[CT]C
BsaI 2539 GAGACC GGTCTC(1/5)
BseAI 118 TCCGGA T^CCGGA
BspEI 118 TCCGGA T^CCGGA
BsrDI 1080 CATTGC GCAATG(2/0)
BssHII 287 GCGCGC G^CGCGC
BstBI 421 TTCGAA TT^CGAA
BstXI 815 CCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 152 CGGACCG CG^G[AT]CCG
DraI 3028 TTTAAA TTT^AAA
DraIII 1402 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1525 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1130 AGCGCT AGC^GCT
EcoRI 3476 GAATTC G^AATTC
EcoRV 3657 GATATC GAT^ATC
EcoT22I 3133 ATGCAT ATGCA^T
FspI 242 TGCGCA TGC^GCA
Hin1I 29 GGCGCC G[GA]^CG[CT]C
HincII 2809 GTCAAC GT[CT]^[GA]AC
HindII 2809 GTCAAC GT[CT]^[GA]AC
KasI 29 GGCGCC G^GCGCC
KpnI 2594 GGTACC GGTAC^C
MroI 118 TCCGGA T^CCGGA
NarI 29 GGCGCC GG^CGCC
NsiI 3133 ATGCAT ATGCA^T
NspV 421 TTCGAA TT^CGAA
PaeR7I 494 CTCGAG C^TCGAG
PflFI 214 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2143 CACGTG CAC^GTG
PmlI 2143 CACGTG CAC^GTG
RsrII 152 CGGACCG CG^G[AT]CCG
SexAI 3357 ACCAGGT A^CC[AT]GGT
SfoI 29 GGCGCC GGC^GCC
SfuI 421 TTCGAA TT^CGAA
SgrAI 31 CGCCGGCG C[GA]^CCGG[CT]G
Tth111I 214 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 815 CCACTGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 494 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 258 CCTGAGG CC^T[ACGT]AGG
3318 CCTCAGG
Asp700I 2382 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2469 GAAAGTCTTC
AvrII 2559 CCTAGG C^CTAGG
2983 CCTAGG
BcgI 2849 CGACCGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2573 GCACCCCGCTCG
BglI 131 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
294 GCCGCCTCGGC
BglII 1013 AGATCT A^GATCT
1706 AGATCT
BlnI 2559 CCTAGG C^CTAGG
2983 CCTAGG
BsaAI 484 TACGTG [CT]AC^GT[GA]
2143 CACGTG
BsaWI 118 TCCGGA [AT]^CCGG[AT]
2456 ACCGGA
BsmI 236 GAATGC GAATGC(1/-1)
2794 GAATGC
Bsp1407I 1587 TGTACA T^GTACA
1820 TGTACA
BsrGI 1587 TGTACA T^GTACA
1820 TGTACA
BssSI 3418 CACGAG CACGAG(-5/-1)
3578 CACGAG
BstAPI 1731 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3076 GCATGGCATGC
Bsu36I 258 CCTGAGG CC^T[ACGT]AGG
3318 CCTCAGG
EagI 185 CGGCCG C^GGCCG
1647 CGGCCG
EclXI 185 CGGCCG C^GGCCG
1647 CGGCCG
Eco52I 185 CGGCCG C^GGCCG
1647 CGGCCG
Eco81I 258 CCTGAGG CC^T[ACGT]AGG
3318 CCTCAGG
NcoI 280 CCATGG C^CATGG
1802 CCATGG
NspI 1544 GCATGC [GA]CATG^[CT]
3081 GCATGC
SapI 400 GAAGAGC GCTCTTC(1/4)
2834 GCTCTTC
SphI 1544 GCATGC GCATG^C
3081 GCATGC
SspBI 1587 TGTACA T^GTACA
1820 TGTACA
XmnI 2382 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2469 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI