################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I BamHI BbrPI BciVI BclI BfrI BsaBI BsiEI BsiWI BspDI BspHI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI HpaI KpnI KspI MamI MluI NcoI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SfuI SnaBI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 3791 GACGTC GACGT^C AccIII 376 TCCGGA T^CCGGA AflIII 1060 ACACGT A^C[GA][CT]GT Asp700I 2147 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2728 TGCGCA TGC^GCA AvrII 2924 CCTAGG C^CTAGG BalI 4178 TGGCCA TGG^CCA BbeI 847 GGCGCC GGCGC^C BcgI 1079 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1189 AGATCT A^GATCT BlnI 2924 CCTAGG C^CTAGG BseAI 376 TCCGGA T^CCGGA BspEI 376 TCCGGA T^CCGGA BsrDI 4330 CATTGC GCAATG(2/0) DraI 3283 TTTAAA TTT^AAA DrdI 3241 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 2728 TGCGCA TGC^GCA HindIII 1871 AAGCTT A^AGCTT KasI 847 GGCGCC G^GCGCC MfeI 4079 CAATTG C^AATTG MluNI 4178 TGGCCA TGG^CCA MroI 376 TCCGGA T^CCGGA MscI 4178 TGGCCA TGG^CCA MslI 1651 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 4079 CAATTG C^AATTG NaeI 844 GCCGGC GCC^GGC NarI 847 GGCGCC GG^CGCC NdeI 3352 CATATG CA^TATG NgoMIV 844 GCCGGC G^CCGGC PaeR7I 366 CTCGAG C^TCGAG PshAI 1043 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 3699 GCTCTTC GCTCTTC(1/4) SexAI 1225 ACCAGGT A^CC[AT]GGT SfiI 2246 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 847 GGCGCC GGC^GCC SgrAI 843 CGCCGGCG C[GA]^CCGG[CT]G SspI 2150 AATATT AAT^ATT XhoI 366 CTCGAG C^TCGAG XmnI 2147 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AclI 1172 AACGTT AA^CGTT 1758 AACGTT AhdI 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1821 GACTCCCAGTC AspEI 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1821 GACTCCCAGTC AspI 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2904 GACAGGGTC BlpI 1179 GCTGAGC GC^T[ACGT]AGC 2571 GCTCAGC Bpu1102I 1179 GCTGAGC GC^T[ACGT]AGC 2571 GCTCAGC BsaAI 1061 CACGTA [CT]AC^GT[GA] 1716 TACGTG BsmBI 1735 CGTCTC CGTCTC(1/5) 2461 CGTCTC BsmI 819 GCATTC GAATGC(1/-1) 2061 GCATTC BsrBI 477 GAGCGG CCGCTC(-3/-3) 1305 GAGCGG BssHII 134 GCGCGC G^CGCGC 428 GCGCGC BssSI 651 CACGAG CACGAG(-5/-1) 3482 CACGAG CelII 1179 GCTGAGC GC^T[ACGT]AGC 2571 GCTCAGC EaeI 223 TGGCCG [CT]^GGCC[GA] 4178 TGGCCA Eam1105I 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1821 GACTCCCAGTC EcoT22I 87 ATGCAT ATGCA^T 817 ATGCAT HincII 1518 GTTGAC GT[CT]^[GA]AC 2207 GTTGAC HindII 1518 GTTGAC GT[CT]^[GA]AC 2207 GTTGAC NsiI 87 ATGCAT ATGCA^T 817 ATGCAT NspI 1942 ACATGC [GA]CATG^[CT] 3506 GCATGT PflFI 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2904 GACAGGGTC PflMI 851 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2667 CCACTGCGTGG Psp1406I 1172 AACGTT AA^CGTT 1758 AACGTT Tth111I 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 2904 GACAGGGTC Van91I 851 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2667 CCACTGCGTGG XbaI 1601 TCTAGA T^CTAGA 2706 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI