##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflIII
AgeI AhdI ApaI AscI Asp718I
AspEI AviII BbeI BciVI BglI
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoT22I FauI FseI
FspI HgaI Hin1I KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PinAI PmeI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 3992 GTAGAC GT^[AC][GT]AC
AclI 1577 AACGTT AA^CGTT
AflII 1591 CTTAAG C^TTAAG
Aor51HI 3673 AGCGCT AGC^GCT
AseI 3864 ATTAAT AT^TAAT
AsnI 3864 ATTAAT AT^TAAT
AspI 3436 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3752 CCTAGG C^CTAGG
BbrPI 3559 CACGTG CAC^GTG
BbsI 76 GAAGAC GAAGAC(2/6)
BcgI 1451 CGATGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1404 TGATCA T^GATCA
BfrI 1591 CTTAAG C^TTAAG
BlnI 3752 CCTAGG C^CTAGG
BlpI 2303 GCTCAGC GC^T[ACGT]AGC
BpmI 1874 CTGGAG CTGGAG(16/14)
Bpu1102I 2303 GCTCAGC GC^T[ACGT]AGC
BpuAI 76 GAAGAC GAAGAC(2/6)
BsaAI 3559 CACGTG [CT]AC^GT[GA]
BsaWI 282 TCCGGT [AT]^CCGG[AT]
BsmI 3046 GAATGC GAATGC(1/-1)
Bsp1407I 737 TGTACA T^GTACA
BspDI 1166 ATCGAT AT^CGAT
BsrFI 937 GCCGGT [GA]^CCGG[CT]
BsrGI 737 TGTACA T^GTACA
BstAPI 3492 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2303 GCTCAGC GC^T[ACGT]AGC
Cfr10I 937 GCCGGT [GA]^CCGG[CT]
ClaI 1166 ATCGAT AT^CGAT
DraIII 967 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3673 AGCGCT AGC^GCT
EcoRV 2346 GATATC GAT^ATC
FbaI 1404 TGATCA T^GATCA
HaeII 3673 AGCGCT [GA]GCGC^[CT]
HindIII 1857 AAGCTT A^AGCTT
HpaI 2600 GTTAAC GTT^AAC
NcoI 1202 CCATGG C^CATGG
NheI 1968 GCTAGC G^CTAGC
PacI 2007 TTAATTAA TTAAT^TAA
PaeR7I 3112 CTCGAG C^TCGAG
PflFI 3436 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3559 CACGTG CAC^GTG
PmlI 3559 CACGTG CAC^GTG
PshAI 3110 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3864 ATTAAT AT^TAAT
Psp1406I 1577 AACGTT AA^CGTT
PvuII 133 CAGCTG CAG^CTG
SacI 184 GAGCTC GAGCT^C
SmaI 1638 CCCGGG CCC^GGG
SphI 1362 GCATGC GCATG^C
SspBI 737 TGTACA T^GTACA
SwaI 1987 ATTTAAAT ATTT^AAAT
Tth111I 3436 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3112 CTCGAG C^TCGAG
XmaCI 1638 CCCGGG C^CCGGG
XmaI 1638 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 40 CGCG CG^CG
3700 CGCG
Alw44I 1179 GTGCAC G^TGCAC
2978 GTGCAC
AlwNI 1342 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1501 CAGCTTCTG
AocI 1347 CCTGAGG CC^T[ACGT]AGG
2656 CCTAAGG
ApaLI 1179 GTGCAC G^TGCAC
2978 GTGCAC
BalI 358 TGGCCA TGG^CCA
2037 TGGCCA
BbvCI 3528 CCTCAGC CCTCAGC(-5/-2)
1140 GCTGAGG
BcnI 1638 CCCGG CC^[GC]GG
1639 CCGGG
BglII 270 AGATCT A^GATCT
830 AGATCT
BsaBI 703 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3629 GATCTCGATC
BspHI 699 TCATGA T^CATGA
1260 TCATGA
BssSI 2924 CTCGTG CACGAG(-5/-1)
3644 CTCGTG
BstUI 40 CGCG CG^CG
3700 CGCG
Bsu36I 1347 CCTGAGG CC^T[ACGT]AGG
2656 CCTAAGG
DsaI 1202 CCATGG C^C[GA][CT]GG
1286 CCGTGG
Eco81I 1347 CCTGAGG CC^T[ACGT]AGG
2656 CCTAAGG
EcoNI 648 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2968 CCTATTGAAGG
EcoRI 115 GAATTC G^AATTC
2168 GAATTC
HincII 1196 GTCAAC GT[CT]^[GA]AC
2600 GTTAAC
HindII 1196 GTCAAC GT[CT]^[GA]AC
2600 GTTAAC
MamI 703 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3629 GATCTCGATC
MluNI 358 TGGCCA TGG^CCA
2037 TGGCCA
MscI 358 TGGCCA TGG^CCA
2037 TGGCCA
MvnI 40 CGCG CG^CG
3700 CGCG
NciI 1638 CCCGG CC^[GC]GG
1639 CCGGG
RcaI 699 TCATGA T^CATGA
1260 TCATGA
SexAI 248 ACCTGGT A^CC[AT]GGT
852 ACCTGGT
SspI 3942 AATATT AAT^ATT
4027 AATATT
TfiI 2680 GATTC G^A[AT]TC
3132 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanI BanII BbvI BfaI
BmyI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI