##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI AviII BamHI BbrPI BciVI
BclI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BstBI BstEII BstPI ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI MamI MluI
MroI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SfuI SgrAI SnaBI
SpeI SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 284 GACGTC GACGT^C
Acc65I 381 GGTACC G^GTACC
AflII 2333 CTTAAG C^TTAAG
AflIII 126 ACACGT A^C[GA][CT]GT
Asp718I 381 GGTACC G^GTACC
BalI 547 TGGCCA TGG^CCA
BfrI 2333 CTTAAG C^TTAAG
BsiEI 75 CGGCCG CG[GA][CT]^CG
BssSI 419 CACGAG CACGAG(-5/-1)
BstXI 580 CCAGTCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 75 CGGCCG C^GGCCG
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
EcoRI 140 GAATTC G^AATTC
HindIII 1909 AAGCTT A^AGCTT
KpnI 381 GGTACC GGTAC^C
KspI 698 CCGCGG CCGC^GG
MfeI 3390 CAATTG C^AATTG
MluNI 547 TGGCCA TGG^CCA
MscI 547 TGGCCA TGG^CCA
MslI 809 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3390 CAATTG C^AATTG
NcoI 528 CCATGG C^CATGG
NheI 3380 GCTAGC G^CTAGC
PshAI 2985 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 698 CCGCGG CCGC^GG
SbfI 2460 CCTGCAGG CCTGCA^GG
ScaI 861 AGTACT AGT^ACT
SexAI 1290 ACCAGGT A^CC[AT]GGT
SfiI 526 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1108 GCATGC GCATG^C
Sse8387I 2460 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 699 CGCG CG^CG
2188 CGCG
AcsI 140 GAATTC [GA]^AATT[CT]
1315 AAATTT
AlwI 619 GATCC GGATC(4/5)
1853 GGATC
ApoI 140 GAATTC [GA]^AATT[CT]
1315 AAATTT
AspI 22 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAGTGTC
AvrII 1150 CCTAGG C^CTAGG
2497 CCTAGG
BcgI 56 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1662 GCATCACCATCG
BglII 900 AGATCT A^GATCT
2486 AGATCT
BlnI 1150 CCTAGG C^CTAGG
2497 CCTAGG
BlpI 3069 GCTTAGC GC^T[ACGT]AGC
3730 GCTCAGC
Bpu1102I 3069 GCTTAGC GC^T[ACGT]AGC
3730 GCTCAGC
BsaAI 127 CACGTA [CT]AC^GT[GA]
977 CACGTA
BsaI 825 GAGACC GGTCTC(1/5)
3058 GGTCTC
BsmBI 786 CGTCTC CGTCTC(1/5)
849 GAGACG
BsmI 1516 GAATGC GAATGC(1/-1)
3473 GCATTC
BsrFI 240 GCCGGT [GA]^CCGG[CT]
750 ACCGGC
Bst1107I 2583 GTATAC GTA^TAC
2609 GTATAC
BstUI 699 CGCG CG^CG
2188 CGCG
BstZ17I 2583 GTATAC GTA^TAC
2609 GTATAC
CelII 3069 GCTTAGC GC^T[ACGT]AGC
3730 GCTCAGC
Cfr10I 240 GCCGGT [GA]^CCGG[CT]
750 ACCGGC
MvnI 699 CGCG CG^CG
2188 CGCG
PflFI 22 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAGTGTC
SacI 1690 GAGCTC GAGCT^C
2390 GAGCTC
TfiI 408 GAATC G^A[AT]TC
2817 GAATC
Tsp45I 999 GTGAC ^GT[GC]AC
2455 GTGAC
Tth111I 22 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2650 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwNI AocI ApaI Asp700I AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspBI
StuI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI