##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI BbrPI BciVI BfrI BglI
BglII BsaAI BseAI BsiWI BspDI
BspEI BsrDI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
FseI HincII HindII HpaI KpnI
MfeI MroI MunI NdeI NheI
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1815 GACGTC GACGT^C
AccI 2885 GTAGAC GT^[AC][GT]AC
AgeI 1439 ACCGGT A^CCGGT
Alw44I 2529 GTGCAC G^TGCAC
ApaI 500 GGGCCC GGGCC^C
ApaLI 2529 GTGCAC G^TGCAC
AviII 2817 TGCGCA TGC^GCA
AvrII 3024 CCTAGG C^CTAGG
BamHI 1835 GGATCC G^GATCC
BbvCI 1387 CCTCAGC CCTCAGC(-5/-2)
BcgI 2864 CGAAGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3547 TGATCA T^GATCA
BlnI 3024 CCTAGG C^CTAGG
BsaBI 2326 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3827 GAGACC GGTCTC(1/5)
BsaWI 1439 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3948 TGTACA T^GTACA
BspHI 1071 TCATGA T^CATGA
BsrBI 862 CCGCTC CCGCTC(-3/-3)
BsrGI 3948 TGTACA T^GTACA
BssHII 93 GCGCGC G^CGCGC
BssSI 1547 CACGAG CACGAG(-5/-1)
EcoT22I 3739 ATGCAT ATGCA^T
FbaI 3547 TGATCA T^GATCA
FspI 2817 TGCGCA TGC^GCA
HindIII 3486 AAGCTT A^AGCTT
MamI 2326 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 418 ACGCGT A^CGCGT
NaeI 3370 GCCGGC GCC^GGC
NgoMIV 3370 GCCGGC G^CCGGC
NsiI 3739 ATGCAT ATGCA^T
PaeR7I 1840 CTCGAG C^TCGAG
PflMI 1098 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1439 ACCGGT A^CCGGT
RcaI 1071 TCATGA T^CATGA
SacI 845 GAGCTC GAGCT^C
SspBI 3948 TGTACA T^GTACA
Van91I 1098 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1891 CCAGCCCTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1840 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Aor51HI 612 AGCGCT AGC^GCT
1756 AGCGCT
Asp700I 1075 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAATCTTTTC
BbsI 962 GAAGAC GAAGAC(2/6)
1471 GAAGAC
BpuAI 962 GAAGAC GAAGAC(2/6)
1471 GAAGAC
BsmBI 2424 CGTCTC CGTCTC(1/5)
3220 CGTCTC
BsmI 1747 GAATGC GAATGC(1/-1)
2213 GAATGC
BspLU11I 1780 ACATGT A^CATGT
3604 ACATGT
BsrFI 1439 ACCGGT [GA]^CCGG[CT]
3370 GCCGGC
Cfr10I 1439 ACCGGT [GA]^CCGG[CT]
3370 GCCGGC
Eco47III 612 AGCGCT AGC^GCT
1756 AGCGCT
KspI 123 CCGCGG CCGC^GG
242 CCGCGG
PshAI 1535 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2418 GACTAACGTC
SacII 123 CCGCGG CCGC^GG
242 CCGCGG
SapI 1570 GAAGAGC GCTCTTC(1/4)
184 GCTCTTC
XmnI 1075 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI