##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
ApaI AscI AspI AvrII BamHI
BbvCI BcgI BcnI BfrI BglI
BlnI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstZ17I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I FseI MfeI MluI MroI
MunI NaeI NciI NgoMIV NheI
NotI NruI PacI PflFI PmeI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1822 GGTACC G^GTACC
AgeI 4084 ACCGGT A^CCGGT
AhdI 1672 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 36 GTGCAC G^TGCAC
ApaLI 36 GTGCAC G^TGCAC
Asp718I 1822 GGTACC G^GTACC
AspEI 1672 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3487 TGCGCA TGC^GCA
BbeI 7 GGCGCC GGCGC^C
BclI 4131 TGATCA T^GATCA
BsaBI 1070 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 180 CGGTCG CG[GA][CT]^CG
BsmBI 4964 GAGACG CGTCTC(1/5)
BspHI 2692 TCATGA T^CATGA
BsrBI 4958 GAGCGG CCGCTC(-3/-3)
BstBI 4500 TTCGAA TT^CGAA
BstEII 4711 GGTGACC G^GT[ACGT]ACC
BstPI 4711 GGTGACC G^GT[ACGT]ACC
Eam1105I 1672 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1279 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4711 GGTGACC G^GT[ACGT]ACC
EcoRV 590 GATATC GAT^ATC
EcoT22I 5810 ATGCAT ATGCA^T
FbaI 4131 TGATCA T^GATCA
FspI 3487 TGCGCA TGC^GCA
HaeII 7 GGCGCC [GA]GCGC^[CT]
HindIII 2029 AAGCTT A^AGCTT
KasI 7 GGCGCC G^GCGCC
KpnI 1822 GGTACC GGTAC^C
MamI 1070 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 7 GGCGCC GG^CGCC
NdeI 1018 CATATG CA^TATG
NsiI 5810 ATGCAT ATGCA^T
NspV 4500 TTCGAA TT^CGAA
PaeR7I 3342 CTCGAG C^TCGAG
PinAI 4084 ACCGGT A^CCGGT
PshAI 4966 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2692 TCATGA T^CATGA
SfoI 7 GGCGCC GGC^GCC
SfuI 4500 TTCGAA TT^CGAA
SphI 5808 GCATGC GCATG^C
XbaI 1421 TCTAGA T^CTAGA
XhoI 3342 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 4271 GTAGAC GT^[AC][GT]AC
4427 GTCTAC
AocI 686 CCTGAGG CC^T[ACGT]AGG
2103 CCTTAGG
AseI 337 ATTAAT AT^TAAT
3019 ATTAAT
AsnI 337 ATTAAT AT^TAAT
3019 ATTAAT
BalI 4686 TGGCCA TGG^CCA
5133 TGGCCA
BbrPI 245 CACGTG CAC^GTG
1717 CACGTG
BlpI 2857 GCTCAGC GC^T[ACGT]AGC
5381 GCTGAGC
Bpu1102I 2857 GCTCAGC GC^T[ACGT]AGC
5381 GCTGAGC
BsaWI 178 TCCGGT [AT]^CCGG[AT]
4084 ACCGGT
Bsp1407I 1090 TGTACA T^GTACA
2803 TGTACA
BsrDI 2252 CATTGC GCAATG(2/0)
4921 CATTGC
BsrFI 1617 ACCGGC [GA]^CCGG[CT]
4084 ACCGGT
BsrGI 1090 TGTACA T^GTACA
2803 TGTACA
Bsu36I 686 CCTGAGG CC^T[ACGT]AGG
2103 CCTTAGG
CelII 2857 GCTCAGC GC^T[ACGT]AGC
5381 GCTGAGC
Cfr10I 1617 ACCGGC [GA]^CCGG[CT]
4084 ACCGGT
Eco81I 686 CCTGAGG CC^T[ACGT]AGG
2103 CCTTAGG
FauI 75 CCCGC CCCGC(4/6)
4960 GCGGG
HgaI 27 GACGC GACGC(5/10)
5932 GCGTC
HpaI 2497 GTTAAC GTT^AAC
6181 GTTAAC
KspI 76 CCGCGG CCGC^GG
4938 CCGCGG
MluNI 4686 TGGCCA TGG^CCA
5133 TGGCCA
MscI 4686 TGGCCA TGG^CCA
5133 TGGCCA
NcoI 195 CCATGG C^CATGG
2319 CCATGG
PmaCI 245 CACGTG CAC^GTG
1717 CACGTG
PmlI 245 CACGTG CAC^GTG
1717 CACGTG
PpuMI 408 AGGACCT [GA]G^G[AT]CC[CT]
4384 GGGACCT
PshBI 337 ATTAAT AT^TAAT
3019 ATTAAT
SacII 76 CCGCGG CCGC^GG
4938 CCGCGG
ScaI 3715 AGTACT AGT^ACT
5474 AGTACT
SspBI 1090 TGTACA T^GTACA
2803 TGTACA
XcmI 4674 CCAAACTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5731 CCAGTTGGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BciVI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI