##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AscI AspEI BciVI BfrI BsaBI
BsiWI BsmI BspHI BsrDI BstBI
Eam1105I EcoRI EcoRV HindIII HpaI
KspI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PmeI Psp1406I RcaI SacII SalI
SfuI SnaBI SpeI SspI XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 2637 GACGTC GACGT^C
AseI 7471 ATTAAT AT^TAAT
AsnI 7471 ATTAAT AT^TAAT
Asp700I 4895 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 947 CCTAGG C^CTAGG
BclI 2788 TGATCA T^GATCA
BglII 2071 AGATCT A^GATCT
BlnI 947 CCTAGG C^CTAGG
BspLU11I 772 ACATGT A^CATGT
Bst1107I 4514 GTATAC GTA^TAC
BstZ17I 4514 GTATAC GTA^TAC
DraI 6731 TTTAAA TTT^AAA
EcoT22I 5676 ATGCAT ATGCA^T
FbaI 2788 TGATCA T^GATCA
NdeI 5652 CATATG CA^TATG
NsiI 5676 ATGCAT ATGCA^T
PshAI 2478 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7471 ATTAAT AT^TAAT
PvuI 4939 CGATCG CGAT^CG
ScaI 253 AGTACT AGT^ACT
SgrAI 4259 CACCGGCG C[GA]^CCGG[CT]G
SphI 5674 GCATGC GCATG^C
SwaI 6730 ATTTAAAT ATTT^AAAT
XmnI 4895 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3792 GTCTAC GT^[AC][GT]AC
4514 GTATAC
AccIII 2122 TCCGGA T^CCGGA
4631 TCCGGA
AgeI 4195 ACCGGT A^CCGGT
5617 ACCGGT
Aor51HI 3325 AGCGCT AGC^GCT
3910 AGCGCT
AviII 809 TGCGCA TGC^GCA
7263 TGCGCA
BamHI 3807 GGATCC G^GATCC
5254 GGATCC
BfaI 948 CTAG C^TAG
4725 CTAG
BseAI 2122 TCCGGA T^CCGGA
4631 TCCGGA
Bsp1407I 208 TGTACA T^GTACA
5128 TGTACA
BspDI 393 ATCGAT AT^CGAT
3504 ATCGAT
BspEI 2122 TCCGGA T^CCGGA
4631 TCCGGA
BsrGI 208 TGTACA T^GTACA
5128 TGTACA
BssHII 1763 GCGCGC G^CGCGC
3567 GCGCGC
ClaI 393 ATCGAT AT^CGAT
3504 ATCGAT
CpoI 214 CGGACCG CG^G[AT]CCG
2901 CGGTCCG
DraIII 318 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
582 CACGAGGTG
Eco47III 3325 AGCGCT AGC^GCT
3910 AGCGCT
FseI 3756 GGCCGGCC GGCCGG^CC
4764 GGCCGGCC
FspI 809 TGCGCA TGC^GCA
7263 TGCGCA
HincII 4500 GTTGAC GT[CT]^[GA]AC
5472 GTTGAC
HindII 4500 GTTGAC GT[CT]^[GA]AC
5472 GTTGAC
MaeI 948 CTAG C^TAG
4725 CTAG
MroI 2122 TCCGGA T^CCGGA
4631 TCCGGA
PaeR7I 4377 CTCGAG C^TCGAG
4431 CTCGAG
PinAI 4195 ACCGGT A^CCGGT
5617 ACCGGT
RsrII 214 CGGACCG CG^G[AT]CCG
2901 CGGTCCG
SbfI 3294 CCTGCAGG CCTGCA^GG
4043 CCTGCAGG
SexAI 4922 ACCAGGT A^CC[AT]GGT
5341 ACCTGGT
SfiI 521 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3756 GGCCGGCCGGGCC
Sse8387I 3294 CCTGCAGG CCTGCA^GG
4043 CCTGCAGG
SspBI 208 TGTACA T^GTACA
5128 TGTACA
XhoI 4377 CTCGAG C^TCGAG
4431 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI