##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AclI AcsI AflII AgeI ApoI
AscI AseI AsnI Asp700I BfrI
BsiWI BspDI Bst1107I BstEII BstPI
BstZ17I ClaI DraI DrdI EcoO65I
EcoRI EcoT22I FseI NdeI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I SalI SgrAI
SnaBI SpeI SspI SwaI XhoI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccI 7074 GTAGAC GT^[AC][GT]AC
AccIII 2280 TCCGGA T^CCGGA
AhdI 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 9281 CCTAGG C^CTAGG
BciVI 10754 GTATCC GTATCC(6/5)
BlnI 9281 CCTAGG C^CTAGG
BsaBI 7205 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2280 TCCGGA T^CCGGA
BspEI 2280 TCCGGA T^CCGGA
BspLU11I 10648 ACATGT A^CATGT
BsrDI 481 CATTGC GCAATG(2/0)
BstBI 4596 TTCGAA TT^CGAA
Eam1105I 332 GACCCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 9171 GATATC GAT^ATC
MamI 7205 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 8488 CAATTG C^AATTG
MroI 2280 TCCGGA T^CCGGA
MunI 8488 CAATTG C^AATTG
NheI 6621 GCTAGC G^CTAGC
NspV 4596 TTCGAA TT^CGAA
PshAI 2269 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 110 CGATCG CGAT^CG
SapI 452 GAAGAGC GCTCTTC(1/4)
ScaI 10464 AGTACT AGT^ACT
SfuI 4596 TTCGAA TT^CGAA
XbaI 8087 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 3823 GACGTC GACGT^C
5331 GACGTC
Acc65I 863 GGTACC G^GTACC
4085 GGTACC
Asp718I 863 GGTACC G^GTACC
4085 GGTACC
AspI 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
10504 GACCAGGTC
AviII 6142 TGCGCA TGC^GCA
10338 TGCGCA
BglII 4376 AGATCT A^GATCT
7123 AGATCT
BsaWI 1294 ACCGGA [AT]^CCGG[AT]
2280 TCCGGA
BsmBI 5474 CGTCTC CGTCTC(1/5)
7601 CGTCTC
BsmI 6647 GAATGC GAATGC(1/-1)
6724 GCATTC
CpoI 105 CGGACCG CG^G[AT]CCG
131 CGGACCG
EagI 241 CGGCCG C^GGCCG
5223 CGGCCG
EclXI 241 CGGCCG C^GGCCG
5223 CGGCCG
Eco52I 241 CGGCCG C^GGCCG
5223 CGGCCG
FspI 6142 TGCGCA TGC^GCA
10338 TGCGCA
HpaI 3183 GTTAAC GTT^AAC
5603 GTTAAC
KpnI 863 GGTACC GGTAC^C
4085 GGTACC
MluI 5060 ACGCGT A^CGCGT
5144 ACGCGT
NcoI 2410 CCATGG C^CATGG
2558 CCATGG
PflFI 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
10504 GACCAGGTC
RsrII 105 CGGACCG CG^G[AT]CCG
131 CGGACCG
SphI 4703 GCATGC GCATG^C
8594 GCATGC
Tth111I 1662 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
10504 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 168 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RcaI RsaI SacI SacII
Sau3AI Sau96I SbfI ScrFI SexAI
SfaNI SfcI SfiI SfoI SmaI
SmlI Sse8387I SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI