##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AspI AvrII
BbeI BbvCI BciVI BfrI BlnI
BsiWI BssHII Bst1107I BstBI BstZ17I
CpoI DraI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FseI HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I RsrII
SacII SalI SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2661 TCCGGA T^CCGGA
AcsI 1006 AAATTC [GA]^AATT[CT]
AocI 2386 CCTGAGG CC^T[ACGT]AGG
Aor51HI 342 AGCGCT AGC^GCT
ApoI 1006 AAATTC [GA]^AATT[CT]
BclI 3601 TGATCA T^GATCA
BglII 3843 AGATCT A^GATCT
BsaBI 2734 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2661 TCCGGA T^CCGGA
BsmBI 3510 GAGACG CGTCTC(1/5)
BspEI 2661 TCCGGA T^CCGGA
BspHI 2790 TCATGA T^CATGA
BspLU11I 2718 ACATGT A^CATGT
BssSI 1772 CTCGTG CACGAG(-5/-1)
Bsu36I 2386 CCTGAGG CC^T[ACGT]AGG
DraIII 2570 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 342 AGCGCT AGC^GCT
Eco81I 2386 CCTGAGG CC^T[ACGT]AGG
FbaI 3601 TGATCA T^GATCA
HindIII 84 AAGCTT A^AGCTT
MamI 2734 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2661 TCCGGA T^CCGGA
PshAI 4072 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1821 CGATCG CGAT^CG
RcaI 2790 TCATGA T^CATGA
SacI 3396 GAGCTC GAGCT^C
ScaI 2901 AGTACT AGT^ACT
SfiI 3962 GGCCAAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 678 CCCGGG CCC^GGG
Tsp509I 1007 AATT ^AATT
XmaCI 678 CCCGGG C^CCGGG
XmaI 678 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 698 GTCTAC GT^[AC][GT]AC
1439 GTCTAC
AflIII 1428 ACGTGT A^C[GA][CT]GT
2718 ACATGT
AhdI 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1574 GACCGCGAGTC
Alw44I 629 GTGCAC G^TGCAC
3426 GTGCAC
ApaLI 629 GTGCAC G^TGCAC
3426 GTGCAC
Asp700I 1858 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2653 GAATCTTTTC
AspEI 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1574 GACCGCGAGTC
AviII 2277 TGCGCA TGC^GCA
2548 TGCGCA
BfaI 2042 CTAG C^TAG
3639 CTAG
Bsp1407I 1593 TGTACA T^GTACA
2812 TGTACA
BspDI 1679 ATCGAT AT^CGAT
2627 ATCGAT
BsrBI 3500 CCGCTC CCGCTC(-3/-3)
443 GAGCGG
BsrGI 1593 TGTACA T^GTACA
2812 TGTACA
BstEII 526 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
BstPI 526 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
ClaI 1679 ATCGAT AT^CGAT
2627 ATCGAT
DrdI 2396 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4151 GACAAGAAAGTC
Eam1105I 662 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1574 GACCGCGAGTC
EarI 2291 CTCTTC CTCTTC(1/4)
1853 GAAGAG
EcoO65I 526 GGTCACC G^GT[ACGT]ACC
1228 GGTGACC
FspI 2277 TGCGCA TGC^GCA
2548 TGCGCA
HaeII 342 AGCGCT [GA]GCGC^[CT]
1566 AGCGCC
HincII 377 GTCAAC GT[CT]^[GA]AC
1165 GTTGAC
HindII 377 GTCAAC GT[CT]^[GA]AC
1165 GTTGAC
Ksp632I 2291 CTCTTC CTCTTC(1/4)
1853 GAAGAG
MaeI 2042 CTAG C^TAG
3639 CTAG
NcoI 2253 CCATGG C^CATGG
3043 CCATGG
NruI 1000 TCGCGA TCG^CGA
3593 TCGCGA
SexAI 546 ACCTGGT A^CC[AT]GGT
2001 ACCTGGT
SspBI 1593 TGTACA T^GTACA
2812 TGTACA
XmnI 1858 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2653 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvI BcgI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII