##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AviII AvrII BcgI
BciVI BlnI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BssHII
BstBI BstUI ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HindIII HpaI
KpnI KspI MluI MroI MvnI
NdeI NotI NruI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 2664 CTTAAG C^TTAAG
ApaI 2966 GGGCCC GGGCC^C
AseI 882 ATTAAT AT^TAAT
AsnI 882 ATTAAT AT^TAAT
AspI 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 3517 TGATCA T^GATCA
BfrI 2664 CTTAAG C^TTAAG
BlpI 3046 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3046 GCTCAGC GC^T[ACGT]AGC
BseRI 3375 GAGGAG GAGGAG(10/8)
BsmBI 2438 CGTCTC CGTCTC(1/5)
Bsp1407I 956 TGTACA T^GTACA
BspLU11I 4084 ACATGT A^CATGT
BsrDI 3774 CATTGC GCAATG(2/0)
BsrGI 956 TGTACA T^GTACA
BssSI 3080 CTCGTG CACGAG(-5/-1)
Bst1107I 159 GTATAC GTA^TAC
BstAPI 521 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 371 GGTGACC G^GT[ACGT]ACC
BstPI 371 GGTGACC G^GT[ACGT]ACC
BstZ17I 159 GTATAC GTA^TAC
CelII 3046 GCTCAGC GC^T[ACGT]AGC
EcoO65I 371 GGTGACC G^GT[ACGT]ACC
FbaI 3517 TGATCA T^GATCA
MfeI 2806 CAATTG C^AATTG
MunI 2806 CAATTG C^AATTG
NaeI 2115 GCCGGC GCC^GGC
NgoMIV 2115 GCCGGC G^CCGGC
NheI 1599 GCTAGC G^CTAGC
PaeR7I 4314 CTCGAG C^TCGAG
PflFI 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4005 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 882 ATTAAT AT^TAAT
ScaI 4348 AGTACT AGT^ACT
SexAI 1813 ACCTGGT A^CC[AT]GGT
SfiI 17 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 4431 GCATGC GCATG^C
SspBI 956 TGTACA T^GTACA
SspI 1522 AATATT AAT^ATT
StuI 4020 AGGCCT AGG^CCT
Tth111I 1398 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4151 TCTAGA T^CTAGA
XhoI 4314 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 159 GTATAC GT^[AC][GT]AC
3560 GTCTAC
AflIII 1852 ACGTGT A^C[GA][CT]GT
4084 ACATGT
Alw44I 2556 GTGCAC G^TGCAC
4090 GTGCAC
AocI 183 CCTGAGG CC^T[ACGT]AGG
2920 CCTCAGG
ApaLI 2556 GTGCAC G^TGCAC
4090 GTGCAC
BamHI 1490 GGATCC G^GATCC
3003 GGATCC
BbeI 392 GGCGCC GGCGC^C
2399 GGCGCC
BbrPI 2976 CACGTG CAC^GTG
3221 CACGTG
BbsI 1745 GAAGAC GAAGAC(2/6)
732 GTCTTC
BbvCI 1611 GCTGAGG CCTCAGC(-5/-2)
1677 GCTGAGG
BglII 1882 AGATCT A^GATCT
3953 AGATCT
BpuAI 1745 GAAGAC GAAGAC(2/6)
732 GTCTTC
BsaBI 2018 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3423 GATGAATATC
BsrBI 1904 CCGCTC CCGCTC(-3/-3)
1067 GAGCGG
BsrFI 10 GCCGGT [GA]^CCGG[CT]
2115 GCCGGC
Bsu36I 183 CCTGAGG CC^T[ACGT]AGG
2920 CCTCAGG
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
2115 GCCGGC
DraI 3506 TTTAAA TTT^AAA
4174 TTTAAA
DrdI 84 GACAACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2908 GACCTCACAGTC
Eco81I 183 CCTGAGG CC^T[ACGT]AGG
2920 CCTCAGG
EcoNI 128 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3995 CCTTCCCCAGG
EcoRI 838 GAATTC G^AATTC
1895 GAATTC
EcoT22I 3163 ATGCAT ATGCA^T
4188 ATGCAT
HincII 246 GTTGAC GT[CT]^[GA]AC
2510 GTCAAC
HindII 246 GTTGAC GT[CT]^[GA]AC
2510 GTCAAC
KasI 392 GGCGCC G^GCGCC
2399 GGCGCC
MamI 2018 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3423 GATGAATATC
NarI 392 GGCGCC GG^CGCC
2399 GGCGCC
NsiI 3163 ATGCAT ATGCA^T
4188 ATGCAT
PmaCI 2976 CACGTG CAC^GTG
3221 CACGTG
PmlI 2976 CACGTG CAC^GTG
3221 CACGTG
SfoI 392 GGCGCC GGC^GCC
2399 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII