##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AclI AflII AseI AsnI AvrII
BciVI BfrI BlnI BsaBI BsiWI
BsrBI Bst1107I BstZ17I CpoI DraI
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PmeI PshBI Psp1406I PvuI RsrII
SapI SbfI ScaI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2830 GACGTC GACGT^C
AccIII 1959 TCCGGA T^CCGGA
AcsI 1042 GAATTC [GA]^AATT[CT]
AhdI 2947 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 6202 AGCGCT AGC^GCT
ApoI 1042 GAATTC [GA]^AATT[CT]
AscI 281 GGCGCGCC GG^CGCGCC
Asp700I 4491 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2947 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1864 CACGTG CAC^GTG
BclI 7824 TGATCA T^GATCA
BglII 2306 AGATCT A^GATCT
BsaAI 1864 CACGTG [CT]AC^GT[GA]
BseAI 1959 TCCGGA T^CCGGA
Bsp1407I 8864 TGTACA T^GTACA
BspDI 3361 ATCGAT AT^CGAT
BspEI 1959 TCCGGA T^CCGGA
BspHI 6500 TCATGA T^CATGA
BsrGI 8864 TGTACA T^GTACA
BssHII 282 GCGCGC G^CGCGC
BssSI 628 CTCGTG CACGAG(-5/-1)
ClaI 3361 ATCGAT AT^CGAT
DrdI 6802 GACCTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2947 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6202 AGCGCT AGC^GCT
EcoRI 1042 GAATTC G^AATTC
FbaI 7824 TGATCA T^GATCA
FseI 8201 GGCCGGCC GGCCGG^CC
HindIII 6110 AAGCTT A^AGCTT
MroI 1959 TCCGGA T^CCGGA
PaeR7I 5863 CTCGAG C^TCGAG
PmaCI 1864 CACGTG CAC^GTG
PmlI 1864 CACGTG CAC^GTG
RcaI 6500 TCATGA T^CATGA
SalI 2320 GTCGAC G^TCGAC
SexAI 8105 ACCAGGT A^CC[AT]GGT
SgrAI 4009 CACCGGTG C[GA]^CCGG[CT]G
SspBI 8864 TGTACA T^GTACA
SspI 1216 AATATT AAT^ATT
Tsp509I 1043 AATT ^AATT
XhoI 5863 CTCGAG C^TCGAG
XmnI 4491 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1369 GTAGAC GT^[AC][GT]AC
2320 GTCGAC
AgeI 3551 ACCGGT A^CCGGT
4010 ACCGGT
AspI 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACTGCGTC
AviII 1868 TGCGCA TGC^GCA
4245 TGCGCA
BbvCI 5820 CCTCAGC CCTCAGC(-5/-2)
7096 GCTGAGG
BsmBI 4504 GAGACG CGTCTC(1/5)
8940 GAGACG
BstBI 3970 TTCGAA TT^CGAA
6463 TTCGAA
EcoRV 3103 GATATC GAT^ATC
5926 GATATC
EcoT22I 3993 ATGCAT ATGCA^T
8275 ATGCAT
FspI 1868 TGCGCA TGC^GCA
4245 TGCGCA
MseI 3533 TTAA T^TAA
4403 TTAA
NsiI 3993 ATGCAT ATGCA^T
8275 ATGCAT
NspV 3970 TTCGAA TT^CGAA
6463 TTCGAA
PflFI 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACTGCGTC
PinAI 3551 ACCGGT A^CCGGT
4010 ACCGGT
PshAI 2607 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3109 GACGAGTGTC
SfuI 3970 TTCGAA TT^CGAA
6463 TTCGAA
Tru9I 3533 TTAA T^TAA
4403 TTAA
Tth111I 1405 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4753 GACTGCGTC
XbaI 2433 TCTAGA T^CTAGA
8217 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 159 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI