##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AclI AhdI ApaI AscI
AspEI AvrII BbeI BbvCI BglII
BlnI BsiWI BssHII BstXI CpoI
Eam1105I EcoRI EcoT22I FseI HindIII
KasI MluI NarI NdeI NheI
NotI NruI NsiI PacI PmeI
PshAI Psp1406I PvuI RsrII SalI
SfiI SfoI SgrAI SnaBI SphI
SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 3611 GGTACC G^GTACC
AccI 4017 GTATAC GT^[AC][GT]AC
AccIII 910 TCCGGA T^CCGGA
AcyI 710 GGCGTC G[GA]^CG[CT]C
AflII 5328 CTTAAG C^TTAAG
AseI 5964 ATTAAT AT^TAAT
AsnI 5964 ATTAAT AT^TAAT
Asp718I 3611 GGTACC G^GTACC
AviII 5632 TGCGCA TGC^GCA
BcgI 1346 GCATAAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5486 TGATCA T^GATCA
BfrI 5328 CTTAAG C^TTAAG
BglI 4061 GCCAGTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 5265 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 710 GGCGTC G[GA]^CG[CT]C
BsaI 214 GAGACC GGTCTC(1/5)
BseAI 910 TCCGGA T^CCGGA
BspEI 910 TCCGGA T^CCGGA
BspLU11I 6265 ACATGT A^CATGT
BsrBI 297 GAGCGG CCGCTC(-3/-3)
BssSI 85 CTCGTG CACGAG(-5/-1)
Bst1107I 4017 GTATAC GTA^TAC
BstAPI 5858 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1354 TTCGAA TT^CGAA
BstZ17I 4017 GTATAC GTA^TAC
DrdI 2808 GACCCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2440 CGGCCG C^GGCCG
EclXI 2440 CGGCCG C^GGCCG
Eco52I 2440 CGGCCG C^GGCCG
EcoRV 2882 GATATC GAT^ATC
FbaI 5486 TGATCA T^GATCA
FspI 5632 TGCGCA TGC^GCA
Hin1I 710 GGCGTC G[GA]^CG[CT]C
KpnI 3611 GGTACC GGTAC^C
MamI 5265 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6108 CAATTG C^AATTG
MroI 910 TCCGGA T^CCGGA
MunI 6108 CAATTG C^AATTG
NaeI 2442 GCCGGC GCC^GGC
NgoMIV 2442 GCCGGC G^CCGGC
NspV 1354 TTCGAA TT^CGAA
PshBI 5964 ATTAAT AT^TAAT
SbfI 4891 CCTGCAGG CCTGCA^GG
ScaI 5751 AGTACT AGT^ACT
SfuI 1354 TTCGAA TT^CGAA
SmaI 249 CCCGGG CCC^GGG
SpeI 3465 ACTAGT A^CTAGT
Sse8387I 4891 CCTGCAGG CCTGCA^GG
StuI 2714 AGGCCT AGG^CCT
XbaI 452 TCTAGA T^CTAGA
XcmI 3686 CCAGTTTTCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 249 CCCGGG C^CCGGG
XmaI 249 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AgeI 138 ACCGGT A^CCGGT
3156 ACCGGT
Alw44I 657 GTGCAC G^TGCAC
3160 GTGCAC
AocI 540 CCTCAGG CC^T[ACGT]AGG
1122 CCTCAGG
ApaLI 657 GTGCAC G^TGCAC
3160 GTGCAC
AspI 1869 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACGCGGTC
BalI 577 TGGCCA TGG^CCA
4059 TGGCCA
BamHI 862 GGATCC G^GATCC
1024 GGATCC
BciVI 2780 GGATAC GTATCC(6/5)
5609 GTATCC
BsiEI 2436 CGGTCG CG[GA][CT]^CG
2440 CGGCCG
BsmI 3592 GCATTC GAATGC(1/-1)
5837 GCATTC
BspDI 1142 ATCGAT AT^CGAT
4518 ATCGAT
BspHI 3598 TCATGA T^CATGA
6018 TCATGA
BsrDI 5629 CATTGC GCAATG(2/0)
2904 GCAATG
Bsu36I 540 CCTCAGG CC^T[ACGT]AGG
1122 CCTCAGG
ClaI 1142 ATCGAT AT^CGAT
4518 ATCGAT
DraIII 195 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
1566 CACCGTGTG
Eco81I 540 CCTCAGG CC^T[ACGT]AGG
1122 CCTCAGG
HpaI 4729 GTTAAC GTT^AAC
5951 GTTAAC
MluNI 577 TGGCCA TGG^CCA
4059 TGGCCA
MscI 577 TGGCCA TGG^CCA
4059 TGGCCA
PaeR7I 219 CTCGAG C^TCGAG
2588 CTCGAG
PflFI 1869 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACGCGGTC
PflMI 2046 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4420 CCAGGTTTTGG
PinAI 138 ACCGGT A^CCGGT
3156 ACCGGT
PpuMI 1126 AGGACCT [GA]G^G[AT]CC[CT]
4887 GGGTCCT
RcaI 3598 TCATGA T^CATGA
6018 TCATGA
SexAI 3607 ACCAGGT A^CC[AT]GGT
4419 ACCAGGT
Tth111I 1869 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2432 GACGCGGTC
Van91I 2046 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4420 CCAGGTTTTGG
XhoI 219 CTCGAG C^TCGAG
2588 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI