##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AvrII BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I EcoT22I
FseI HpaI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2291 GGTACC G^GTACC
AccI 2197 GTCGAC GT^[AC][GT]AC
AcsI 321 GAATTC [GA]^AATT[CT]
AflII 5428 CTTAAG C^TTAAG
AflIII 1908 ACATGT A^C[GA][CT]GT
Alw44I 5891 GTGCAC G^TGCAC
ApaLI 5891 GTGCAC G^TGCAC
ApoI 321 GAATTC [GA]^AATT[CT]
Asp718I 2291 GGTACC G^GTACC
AspI 5098 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5939 CACGTG CAC^GTG
BclI 3305 TGATCA T^GATCA
BfrI 5428 CTTAAG C^TTAAG
BglII 3518 AGATCT A^GATCT
BsaAI 5939 CACGTG [CT]AC^GT[GA]
BsiEI 3152 CGGCCG CG[GA][CT]^CG
BsmI 3401 GCATTC GAATGC(1/-1)
BspLU11I 1908 ACATGT A^CATGT
DraI 1656 TTTAAA TTT^AAA
EagI 3152 CGGCCG C^GGCCG
EclXI 3152 CGGCCG C^GGCCG
Eco52I 3152 CGGCCG C^GGCCG
EcoRI 321 GAATTC G^AATTC
EcoRV 3534 GATATC GAT^ATC
FbaI 3305 TGATCA T^GATCA
HincII 2197 GTCGAC GT[CT]^[GA]AC
HindII 2197 GTCGAC GT[CT]^[GA]AC
KpnI 2291 GGTACC GGTAC^C
NaeI 5607 GCCGGC GCC^GGC
NgoMIV 5607 GCCGGC G^CCGGC
PflFI 5098 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5939 CACGTG CAC^GTG
PmlI 5939 CACGTG CAC^GTG
SalI 2197 GTCGAC G^TCGAC
SphI 2264 GCATGC GCATG^C
Tth111I 5098 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2239 CGCG CG^CG
4854 CGCG
AccIII 5012 TCCGGA T^CCGGA
5578 TCCGGA
Aor51HI 2180 AGCGCT AGC^GCT
2994 AGCGCT
Asp700I 4083 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4899 GAAGTTGTTC
AviII 4385 TGCGCA TGC^GCA
5459 TGCGCA
BbeI 3222 GGCGCC GGCGC^C
3598 GGCGCC
BcgI 1272 GCAACAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3894 GCAGCAGGCTCG
BciVI 1437 GTATCC GTATCC(6/5)
5009 GTATCC
BsaWI 5012 TCCGGA [AT]^CCGG[AT]
5578 TCCGGA
BseAI 5012 TCCGGA T^CCGGA
5578 TCCGGA
BsmBI 5829 CGTCTC CGTCTC(1/5)
463 GAGACG
BspEI 5012 TCCGGA T^CCGGA
5578 TCCGGA
BsrDI 1477 GCAATG GCAATG(2/0)
3706 GCAATG
BstEII 1561 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
BstPI 1561 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
BstUI 2239 CGCG CG^CG
4854 CGCG
DraIII 2526 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3986 CACAGCGTG
Eco47III 2180 AGCGCT AGC^GCT
2994 AGCGCT
EcoO65I 1561 GGTGACC G^GT[ACGT]ACC
5238 GGTGACC
FspI 4385 TGCGCA TGC^GCA
5459 TGCGCA
KasI 3222 GGCGCC G^GCGCC
3598 GGCGCC
MroI 5012 TCCGGA T^CCGGA
5578 TCCGGA
MvnI 2239 CGCG CG^CG
4854 CGCG
NarI 3222 GGCGCC GG^CGCC
3598 GGCGCC
SacI 2164 GAGCTC GAGCT^C
2827 GAGCTC
SexAI 1640 ACCAGGT A^CC[AT]GGT
2786 ACCTGGT
SfoI 3222 GGCGCC GGC^GCC
3598 GGCGCC
XmnI 4083 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4899 GAAGTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI