##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AviII BamHI BbrPI BbsI BciVI
BclI BfrI BpuAI BsaAI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DrdI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI MluI
MroI NdeI NheI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
SalI SbfI ScaI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4640 GACGTC GACGT^C
Alw44I 4831 GTGCAC G^TGCAC
Aor51HI 2595 AGCGCT AGC^GCT
ApaLI 4831 GTGCAC G^TGCAC
Asp700I 2023 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1958 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2436 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 308 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 308 GCTGAGC GC^T[ACGT]AGC
BsaBI 1593 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 653 TCCGGT [AT]^CCGG[AT]
BsiEI 2 CGGCCG CG[GA][CT]^CG
BspHI 3014 TCATGA T^CATGA
BspLU11I 4874 ACATGT A^CATGT
BssSI 1760 CTCGTG CACGAG(-5/-1)
CelII 308 GCTGAGC GC^T[ACGT]AGC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco47III 2595 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
EcoRI 2519 GAATTC G^AATTC
HincII 1169 GTCAAC GT[CT]^[GA]AC
HindII 1169 GTCAAC GT[CT]^[GA]AC
KspI 1136 CCGCGG CCGC^GG
MamI 1593 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2266 CAATTG C^AATTG
MseI 4498 TTAA T^TAA
MunI 2266 CAATTG C^AATTG
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 1958 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4792 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3014 TCATGA T^CATGA
SacII 1136 CCGCGG CCGC^GG
SexAI 4721 ACCAGGT A^CC[AT]GGT
SgrAI 2713 CGCCGGTG C[GA]^CCGG[CT]G
TaqI 1401 TCGA T^CGA
Tru9I 4498 TTAA T^TAA
Tth111I 1958 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1401 TCGA T^CGA
XbaI 1544 TCTAGA T^CTAGA
XmnI 2023 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 4621 ACACGT A^C[GA][CT]GT
4874 ACATGT
AhdI 4640 GACGTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4720 GACCAGGTGTC
AspEI 4640 GACGTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4720 GACCAGGTGTC
AvrII 1557 CCTAGG C^CTAGG
4225 CCTAGG
BglII 979 AGATCT A^GATCT
1410 AGATCT
BlnI 1557 CCTAGG C^CTAGG
4225 CCTAGG
BsmBI 1982 CGTCTC CGTCTC(1/5)
2709 GAGACG
BstAPI 609 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4328 GCACACGCTGC
CpoI 1311 CGGTCCG CG^G[AT]CCG
2334 CGGACCG
DraIII 1757 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
4161 CACAGTGTG
Eam1105I 4640 GACGTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4720 GACCAGGTGTC
EcoNI 3379 CCTCGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3776 CCTCGCGCAGG
HindIII 588 AAGCTT A^AGCTT
2144 AAGCTT
NaeI 618 GCCGGC GCC^GGC
3290 GCCGGC
NcoI 49 CCATGG C^CATGG
396 CCATGG
NgoMIV 618 GCCGGC G^CCGGC
3290 GCCGGC
RsrII 1311 CGGTCCG CG^G[AT]CCG
2334 CGGACCG
SapI 2550 GAAGAGC GCTCTTC(1/4)
956 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI