##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BpuAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FseI FspI Hin1I KasI KspI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 1586 GGTACC G^GTACC
AflIII 1573 ACATGT A^C[GA][CT]GT
Alw44I 1742 GTGCAC G^TGCAC
AocI 4967 CCTTAGG CC^T[ACGT]AGG
ApaLI 1742 GTGCAC G^TGCAC
Asp718I 1586 GGTACC G^GTACC
AvaI 122 CTCGGG C^[CT]CG[GA]G
BalI 468 TGGCCA TGG^CCA
BamHI 1206 GGATCC G^GATCC
BclI 4676 TGATCA T^GATCA
BsaAI 540 TACGTG [CT]AC^GT[GA]
BsaBI 2041 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 122 CTCGGG C^[CT]CG[GA]G
BspLU11I 1573 ACATGT A^CATGT
BspMI 2618 GCAGGT ACCTGC(4/8)
BssKI 236 CCAGG ^CC[ACGT]GG
BssSI 1862 CTCGTG CACGAG(-5/-1)
Bst1107I 3117 GTATAC GTA^TAC
BstAPI 1328 GCATACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 236 CCAGG CC^[AT]GG
BstXI 149 CCAAATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3117 GTATAC GTA^TAC
Bsu36I 4967 CCTTAGG CC^T[ACGT]AGG
CfoI 1124 GCGC GCG^C
DsaI 2254 CCATGG C^C[GA][CT]GG
Eco81I 4967 CCTTAGG CC^T[ACGT]AGG
EcoRI 262 GAATTC G^AATTC
EcoRII 236 CCAGG ^CC[AT]GG
EcoRV 2045 GATATC GAT^ATC
EcoT22I 1158 ATGCAT ATGCA^T
FbaI 4676 TGATCA T^GATCA
HaeII 1123 GGCGCT [GA]GCGC^[CT]
HapII 138 CCGG C^CGG
HgaI 1343 GCGTC GACGC(5/10)
HhaI 1124 GCGC GCG^C
HinP1I 1124 GCGC G^CGC
HpaI 2241 GTTAAC GTT^AAC
HpaII 138 CCGG C^CGG
KpnI 1586 GGTACC GGTAC^C
MamI 2041 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 468 TGGCCA TGG^CCA
MscI 468 TGGCCA TGG^CCA
MspA1I 1385 CAGCTG C[AC]G^C[GT]G
MspI 138 CCGG C^CGG
MvaI 236 CCAGG CC^[AT]GG
NcoI 2254 CCATGG C^CATGG
NdeI 5291 CATATG CA^TATG
NsiI 1158 ATGCAT ATGCA^T
PvuII 1385 CAGCTG CAG^CTG
SacI 41 GAGCTC GAGCT^C
SapI 1006 GAAGAGC GCTCTTC(1/4)
ScrFI 236 CCAGG CC^[ACGT]GG
SpeI 5193 ACTAGT A^CTAGT
StuI 1287 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 659 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
1136 CAGACTCTG
BsaI 4881 GAGACC GGTCTC(1/5)
3161 GGTCTC
BsmFI 1679 GTCCC GGGAC(10/14)
4382 GTCCC
BsmI 1046 GAATGC GAATGC(1/-1)
2026 GCATTC
Bsp1407I 2414 TGTACA T^GTACA
4569 TGTACA
BspHI 680 TCATGA T^CATGA
1718 TCATGA
BsrGI 2414 TGTACA T^GTACA
4569 TGTACA
DraII 1409 AGGCCCT [GA]G^G[ACGT]CC[CT]
4643 GGGGCCT
EaeI 230 TGGCCG [CT]^GGCC[GA]
468 TGGCCA
EcoO109I 1409 AGGCCCT [GA]G^G[ACGT]CC[CT]
4643 GGGGCCT
HindIII 2639 AAGCTT A^AGCTT
3109 AAGCTT
MfeI 2293 CAATTG C^AATTG
4076 CAATTG
MunI 2293 CAATTG C^AATTG
4076 CAATTG
PshAI 1672 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4883 GACCCCAGTC
PstI 362 CTGCAG CTGCA^G
2616 CTGCAG
RcaI 680 TCATGA T^CATGA
1718 TCATGA
SspBI 2414 TGTACA T^GTACA
4569 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbvI BfaI BfrI BglII BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScaI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI