##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI BbeI BbrPI
BcgI BglI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI HaeII HgaI
HindIII HpaI KasI KspI MamI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 736 GACGTC GACGT^C
AccII 3826 CGCG CG^CG
AclI 3627 AACGTT AA^CGTT
AcyI 736 GACGTC G[GA]^CG[CT]C
AflII 2230 CTTAAG C^TTAAG
AflIII 5266 ACATGT A^C[GA][CT]GT
AlwNI 1020 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2294 ATTAAT AT^TAAT
AsnI 2294 ATTAAT AT^TAAT
AvrII 2301 CCTAGG C^CTAGG
BalI 376 TGGCCA TGG^CCA
BbvCI 5001 GCTGAGG CCTCAGC(-5/-2)
BclI 3810 TGATCA T^GATCA
BfrI 2230 CTTAAG C^TTAAG
BlnI 2301 CCTAGG C^CTAGG
BsaHI 736 GACGTC G[GA]^CG[CT]C
BspHI 3740 TCATGA T^CATGA
BspLU11I 5266 ACATGT A^CATGT
BstUI 3826 CGCG CG^CG
DraIII 4113 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1241 CCTCACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4926 GAATTC G^AATTC
FbaI 3810 TGATCA T^GATCA
Hin1I 736 GACGTC G[GA]^CG[CT]C
MfeI 3064 CAATTG C^AATTG
MluNI 376 TGGCCA TGG^CCA
MscI 376 TGGCCA TGG^CCA
MunI 3064 CAATTG C^AATTG
MvnI 3826 CGCG CG^CG
NdeI 3994 CATATG CA^TATG
NspI 5266 ACATGT [GA]CATG^[CT]
PacI 3077 TTAATTAA TTAAT^TAA
PflMI 2726 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2294 ATTAAT AT^TAAT
Psp1406I 3627 AACGTT AA^CGTT
RcaI 3740 TCATGA T^CATGA
SmaI 5519 CCCGGG CCC^GGG
StuI 283 AGGCCT AGG^CCT
TaqI 1065 TCGA T^CGA
TthHB8I 1065 TCGA T^CGA
Van91I 2726 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 5519 CCCGGG C^CCGGG
XmaI 5519 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 550 GGTACC G^GTACC
5197 GGTACC
AccI 485 GTCTAC GT^[AC][GT]AC
2139 GTAGAC
ApaI 228 GGGCCC GGGCC^C
855 GGGCCC
Asp718I 550 GGTACC G^GTACC
5197 GGTACC
AspI 3744 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5536 GACTCCGTC
AviII 2563 TGCGCA TGC^GCA
4355 TGCGCA
BamHI 794 GGATCC G^GATCC
4397 GGATCC
BbsI 2962 GTCTTC GAAGAC(2/6)
3708 GTCTTC
BciVI 1750 GGATAC GTATCC(6/5)
2850 GTATCC
BpuAI 2962 GTCTTC GAAGAC(2/6)
3708 GTCTTC
BsmBI 916 CGTCTC CGTCTC(1/5)
5541 CGTCTC
BsmI 1085 GAATGC GAATGC(1/-1)
2343 GAATGC
BstAPI 1205 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1300 GCAGGTGATGC
DrdI 1627 GACAATAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4644 GACCAGGTGGTC
FauI 3454 GCGGG CCCGC(4/6)
4532 GCGGG
FspI 2563 TGCGCA TGC^GCA
4355 TGCGCA
KpnI 550 GGTACC GGTAC^C
5197 GGTACC
PflFI 3744 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5536 GACTCCGTC
SacI 341 GAGCTC GAGCT^C
2760 GAGCTC
SapI 2757 GAAGAGC GCTCTTC(1/4)
343 GCTCTTC
SexAI 2814 ACCTGGT A^CC[AT]GGT
4645 ACCAGGT
Tth111I 3744 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5536 GACTCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XcmI XhoII XmnI