##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AscI AspI AvrII BamHI BbeI
BclI BfrI BlnI BsmI BspLU11I
Bst1107I BstBI BstZ17I CpoI DraI
EcoRV EcoT22I FbaI FseI HpaI
KasI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI Psp1406I PvuI RsrII
SalI SbfI SfoI SfuI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 4933 GGTACC G^GTACC
AhdI 2318 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 328 AGCGCT AGC^GCT
AseI 1355 ATTAAT AT^TAAT
AsnI 1355 ATTAAT AT^TAAT
Asp700I 2707 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4933 GGTACC G^GTACC
AspEI 2318 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3721 CACGTG CAC^GTG
BciVI 3032 GTATCC GTATCC(6/5)
BglII 3690 AGATCT A^GATCT
BsaAI 3721 CACGTG [CT]AC^GT[GA]
Bsp1407I 5133 TGTACA T^GTACA
BspDI 1364 ATCGAT AT^CGAT
BsrGI 5133 TGTACA T^GTACA
BssHII 3594 GCGCGC G^CGCGC
ClaI 1364 ATCGAT AT^CGAT
DraIII 1658 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 761 CGGCCG C^GGCCG
Eam1105I 2318 GACCGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 761 CGGCCG C^GGCCG
Eco47III 328 AGCGCT AGC^GCT
Eco52I 761 CGGCCG C^GGCCG
HincII 290 GTCAAC GT[CT]^[GA]AC
HindII 290 GTCAAC GT[CT]^[GA]AC
HindIII 1112 AAGCTT A^AGCTT
KpnI 4933 GGTACC GGTAC^C
MfeI 3252 CAATTG C^AATTG
MluI 259 ACGCGT A^CGCGT
MunI 3252 CAATTG C^AATTG
NaeI 1023 GCCGGC GCC^GGC
NgoMIV 1023 GCCGGC G^CCGGC
PmaCI 3721 CACGTG CAC^GTG
PmlI 3721 CACGTG CAC^GTG
PshBI 1355 ATTAAT AT^TAAT
SexAI 2109 ACCAGGT A^CC[AT]GGT
SfiI 3359 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1022 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4788 GCATGC GCATG^C
SspBI 5133 TGTACA T^GTACA
XbaI 2782 TCTAGA T^CTAGA
XmnI 2707 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 3009 TCCGGA T^CCGGA
4874 TCCGGA
BbvCI 2854 GCTGAGG CCTCAGC(-5/-2)
2861 GCTGAGG
BlpI 1016 GCTGAGC GC^T[ACGT]AGC
1669 GCTGAGC
Bpu1102I 1016 GCTGAGC GC^T[ACGT]AGC
1669 GCTGAGC
BsaBI 1099 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3148 GATGCACATC
BsaI 4469 GAGACC GGTCTC(1/5)
3683 GGTCTC
BsaWI 3009 TCCGGA [AT]^CCGG[AT]
4874 TCCGGA
BseAI 3009 TCCGGA T^CCGGA
4874 TCCGGA
BsiEI 280 CGACCG CG[GA][CT]^CG
761 CGGCCG
BsiWI 182 CGTACG C^GTACG
3452 CGTACG
BspEI 3009 TCCGGA T^CCGGA
4874 TCCGGA
BsrDI 3196 CATTGC GCAATG(2/0)
4275 GCAATG
BstAPI 2804 GCAGTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4322 GCAGGTACTGC
CelII 1016 GCTGAGC GC^T[ACGT]AGC
1669 GCTGAGC
DrdI 281 GACCGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2246 GACGCCATCGTC
KspI 3241 CCGCGG CCGC^GG
4731 CCGCGG
MamI 1099 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3148 GATGCACATC
MroI 3009 TCCGGA T^CCGGA
4874 TCCGGA
NspI 4788 GCATGC [GA]CATG^[CT]
4825 GCATGT
PaeR7I 671 CTCGAG C^TCGAG
3401 CTCGAG
PshAI 99 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1444 GACCATCGTC
PstI 723 CTGCAG CTGCA^G
3183 CTGCAG
SacI 883 GAGCTC GAGCT^C
3005 GAGCTC
SacII 3241 CCGCGG CCGC^GG
4731 CCGCGG
SapI 2888 GAAGAGC GCTCTTC(1/4)
1570 GCTCTTC
ScaI 1269 AGTACT AGT^ACT
2759 AGTACT
SfcI 723 CTGCAG C^T[GA][CT]AG
3183 CTGCAG
StuI 556 AGGCCT AGG^CCT
904 AGGCCT
XhoI 671 CTCGAG C^TCGAG
3401 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI