##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AviII BamHI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstZ17I
ClaI DraIII DrdI EcoRV EcoT22I
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NheI NotI NruI NsiI PacI
PflFI PinAI PmeI PshBI PvuI
SacII SbfI ScaI SexAI SfiI
SgrAI SpeI SphI Sse8387I SspBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 289 GTCGAC GT^[AC][GT]AC
AclI 5452 AACGTT AA^CGTT
AflII 3280 CTTAAG C^TTAAG
AhdI 1579 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 3105 GGATC GGATC(4/5)
AocI 1997 CCTGAGG CC^T[ACGT]AGG
AspEI 1579 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 4727 TGATCA T^GATCA
BfrI 3280 CTTAAG C^TTAAG
BglII 4321 AGATCT A^GATCT
BlpI 2254 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2254 GCTCAGC GC^T[ACGT]AGC
BsaWI 657 ACCGGA [AT]^CCGG[AT]
BspHI 4223 TCATGA T^CATGA
BstBI 849 TTCGAA TT^CGAA
BstEII 1669 GGTCACC G^GT[ACGT]ACC
BstPI 1669 GGTCACC G^GT[ACGT]ACC
Bsu36I 1997 CCTGAGG CC^T[ACGT]AGG
CelII 2254 GCTCAGC GC^T[ACGT]AGC
CpoI 1164 CGGACCG CG^G[AT]CCG
DraI 5465 TTTAAA TTT^AAA
Eam1105I 1579 GACCATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1997 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1669 GGTCACC G^GT[ACGT]ACC
EcoRI 4586 GAATTC G^AATTC
FbaI 4727 TGATCA T^GATCA
NdeI 870 CATATG CA^TATG
NspV 849 TTCGAA TT^CGAA
PaeR7I 1177 CTCGAG C^TCGAG
Psp1406I 5452 AACGTT AA^CGTT
RcaI 4223 TCATGA T^CATGA
RsrII 1164 CGGACCG CG^G[AT]CCG
SalI 289 GTCGAC G^TCGAC
SfuI 849 TTCGAA TT^CGAA
SmaI 2096 CCCGGG CCC^GGG
SnaBI 4804 TACGTA TAC^GTA
XbaI 3024 TCTAGA T^CTAGA
XhoI 1177 CTCGAG C^TCGAG
XmaCI 2096 CCCGGG C^CCGGG
XmaI 2096 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 3605 AAATTT [GA]^AATT[CT]
4586 GAATTC
AflIII 726 ACGTGT A^C[GA][CT]GT
2363 ACGTGT
ApoI 3605 AAATTT [GA]^AATT[CT]
4586 GAATTC
Asp700I 697 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3291 GAACAGTTTC
AvrII 1856 CCTAGG C^CTAGG
4168 CCTAGG
BbeI 386 GGCGCC GGCGC^C
1784 GGCGCC
BbrPI 1079 CACGTG CAC^GTG
4257 CACGTG
BcgI 383 CGAGGCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
841 GCAGCAGGTTCG
BciVI 1864 GGATAC GTATCC(6/5)
4308 GGATAC
BlnI 1856 CCTAGG C^CTAGG
4168 CCTAGG
BsaI 2971 GGTCTC GGTCTC(1/5)
4423 GGTCTC
BsmI 10 GAATGC GAATGC(1/-1)
4050 GCATTC
BsrBI 1648 CCGCTC CCGCTC(-3/-3)
2110 CCGCTC
BsrDI 4266 CATTGC GCAATG(2/0)
1931 GCAATG
BstAPI 1699 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2346 GCAGGCTTTGC
BstYI 3105 GGATCT [GA]^GATC[CT]
4321 AGATCT
HincII 289 GTCGAC GT[CT]^[GA]AC
1576 GTTGAC
HindII 289 GTCGAC GT[CT]^[GA]AC
1576 GTTGAC
HindIII 3619 AAGCTT A^AGCTT
3839 AAGCTT
KasI 386 GGCGCC G^GCGCC
1784 GGCGCC
MflI 3105 GGATCT [GA]^GATC[CT]
4321 AGATCT
NarI 386 GGCGCC GG^CGCC
1784 GGCGCC
NspI 999 GCATGT [GA]CATG^[CT]
3704 ACATGC
PflMI 1224 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3781 CCAGCCCTTGG
PmaCI 1079 CACGTG CAC^GTG
4257 CACGTG
PmlI 1079 CACGTG CAC^GTG
4257 CACGTG
PshAI 19 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3591 GACCCAAGTC
PstI 1711 CTGCAG CTGCA^G
4136 CTGCAG
SapI 2505 GCTCTTC GCTCTTC(1/4)
3530 GCTCTTC
SfoI 386 GGCGCC GGC^GCC
1784 GGCGCC
Van91I 1224 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3781 CCAGCCCTTGG
XcmI 672 CCAGCTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1960 CCAGCCCCTCACTGG
XhoII 3105 GGATCT [GA]^GATC[CT]
4321 AGATCT
XmnI 697 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3291 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I