##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AocI Aor51HI AscI
AseI AsnI AspEI AviII BamHI
BbeI BbsI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
FspI HaeII Hin1I KasI KspI
MamI MluI MroI MvnI NarI
NheI NotI NruI NspV PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 718 GTGCAC G^TGCAC
ApaLI 718 GTGCAC G^TGCAC
BbrPI 3776 CACGTG CAC^GTG
BglII 3289 AGATCT A^GATCT
BsaAI 3776 CACGTG [CT]AC^GT[GA]
BsmI 3910 GAATGC GAATGC(1/-1)
BsrFI 4501 GCCGGC [GA]^CCGG[CT]
CfoI 1667 GCGC GCG^C
Cfr10I 4501 GCCGGC [GA]^CCGG[CT]
DrdI 4442 GACTTAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4204 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2109 GATATC GAT^ATC
EcoT22I 3069 ATGCAT ATGCA^T
HhaI 1667 GCGC GCG^C
HinP1I 1667 GCGC G^CGC
HpaI 4463 GTTAAC GTT^AAC
MfeI 1397 CAATTG C^AATTG
MunI 1397 CAATTG C^AATTG
NaeI 4501 GCCGGC GCC^GGC
NgoMIV 4501 GCCGGC G^CCGGC
NsiI 3069 ATGCAT ATGCA^T
PacI 77 TTAATTAA TTAAT^TAA
PmaCI 3776 CACGTG CAC^GTG
PmlI 3776 CACGTG CAC^GTG
SacI 3187 GAGCTC GAGCT^C
SapI 456 GAAGAGC GCTCTTC(1/4)
SexAI 5020 ACCAGGT A^CC[AT]GGT
SphI 4743 GCATGC GCATG^C
StuI 2307 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2282 GGTACC G^GTACC
3632 GGTACC
AflII 1211 CTTAAG C^TTAAG
4208 CTTAAG
AflIII 450 ACATGT A^C[GA][CT]GT
5176 ACATGT
ApaI 470 GGGCCC GGGCC^C
4391 GGGCCC
Asp700I 3828 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4948 GAAAGTATTC
Asp718I 2282 GGTACC G^GTACC
3632 GGTACC
AspI 479 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2505 GACCCAGTC
AvaI 615 CCCGGG C^[CT]CG[GA]G
1802 CCCGGG
BalI 224 TGGCCA TGG^CCA
1917 TGGCCA
BcgI 2717 CGAGCATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4846 GCAGCTGGCTCG
BciVI 3557 GTATCC GTATCC(6/5)
5096 GTATCC
BclI 3294 TGATCA T^GATCA
3612 TGATCA
BfrI 1211 CTTAAG C^TTAAG
4208 CTTAAG
BlpI 1354 GCTAAGC GC^T[ACGT]AGC
3184 GCTGAGC
Bpu1102I 1354 GCTAAGC GC^T[ACGT]AGC
3184 GCTGAGC
BsmBI 2204 CGTCTC CGTCTC(1/5)
1897 GAGACG
BsmFI 2794 GGGAC GGGAC(10/14)
3989 GGGAC
BsoBI 615 CCCGGG C^[CT]CG[GA]G
1802 CCCGGG
Bsp1407I 2234 TGTACA T^GTACA
5153 TGTACA
BspHI 3795 TCATGA T^CATGA
4942 TCATGA
BspLU11I 450 ACATGT A^CATGT
5176 ACATGT
BsrGI 2234 TGTACA T^GTACA
5153 TGTACA
BstAPI 1789 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3914 GCAGTGGCTGC
BstF5I 3209 CATCC GGATG(2/0)
2437 GGATG
CelII 1354 GCTAAGC GC^T[ACGT]AGC
3184 GCTGAGC
EaeI 224 TGGCCA [CT]^GGCC[GA]
1917 TGGCCA
FauI 4733 CCCGC CCCGC(4/6)
1382 GCGGG
FbaI 3294 TGATCA T^GATCA
3612 TGATCA
FokI 3209 CATCC GGATG(9/13)
2437 GGATG
HgaI 1393 GCGTC GACGC(5/10)
3923 GCGTC
HincII 1165 GTTGAC GT[CT]^[GA]AC
4463 GTTAAC
HindII 1165 GTTGAC GT[CT]^[GA]AC
4463 GTTAAC
HindIII 1583 AAGCTT A^AGCTT
3309 AAGCTT
KpnI 2282 GGTACC GGTAC^C
3632 GGTACC
MluNI 224 TGGCCA TGG^CCA
1917 TGGCCA
MscI 224 TGGCCA TGG^CCA
1917 TGGCCA
PflFI 479 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2505 GACCCAGTC
RcaI 3795 TCATGA T^CATGA
4942 TCATGA
SmaI 615 CCCGGG CCC^GGG
1802 CCCGGG
SspBI 2234 TGTACA T^GTACA
5153 TGTACA
SspI 24 AATATT AAT^ATT
3749 AATATT
TaqI 3952 TCGA T^CGA
4227 TCGA
Tth111I 479 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2505 GACCCAGTC
TthHB8I 3952 TCGA T^CGA
4227 TCGA
XbaI 961 TCTAGA T^CTAGA
2158 TCTAGA
XmaCI 615 CCCGGG C^CCGGG
1802 CCCGGG
XmaI 615 CCCGGG C^CCGGG
1802 CCCGGG
XmnI 3828 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4948 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
AvrII BanI BanII BbvCI BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrDI BsrI BssKI Bst1107I
BstNI BstXI BstYI BstZ17I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII