##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AgeI Alw44I ApaI ApaLI
AscI Asp718I AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BlnI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoNI
EcoO65I EcoT22I FseI FspI HgaI
Hin1I KasI KpnI KspI MamI
MluI MvnI NaeI NarI NgoMIV
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SgrAI SmaI SpeI
SphI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 3573 TCCGGA T^CCGGA
AflIII 4507 ACATGT A^C[GA][CT]GT
AhdI 170 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4186 CCTTAGG CC^T[ACGT]AGG
Aor51HI 4361 AGCGCT AGC^GCT
AspEI 170 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 327 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2647 CTCGAG C^[CT]CG[GA]G
BciVI 2476 GTATCC GTATCC(6/5)
BcnI 3068 CCGGG CC^[GC]GG
BglI 2596 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3657 TACGTA [CT]AC^GT[GA]
BsaWI 3573 TCCGGA [AT]^CCGG[AT]
BseAI 3573 TCCGGA T^CCGGA
BsoBI 2647 CTCGAG C^[CT]CG[GA]G
BspEI 3573 TCCGGA T^CCGGA
BspLU11I 4507 ACATGT A^CATGT
BstBI 1852 TTCGAA TT^CGAA
Bsu36I 4186 CCTTAGG CC^T[ACGT]AGG
Eam1105I 170 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4361 AGCGCT AGC^GCT
Eco81I 4186 CCTTAGG CC^T[ACGT]AGG
EcoRV 3168 GATATC GAT^ATC
HaeII 4361 AGCGCT [GA]GCGC^[CT]
HincII 1573 GTTAAC GT[CT]^[GA]AC
HindII 1573 GTTAAC GT[CT]^[GA]AC
HpaI 1573 GTTAAC GTT^AAC
MfeI 4484 CAATTG C^AATTG
MroI 3573 TCCGGA T^CCGGA
MunI 4484 CAATTG C^AATTG
NciI 3068 CCGGG CC^[GC]GG
NcoI 3730 CCATGG C^CATGG
NheI 1593 GCTAGC G^CTAGC
NspV 1852 TTCGAA TT^CGAA
PaeR7I 2647 CTCGAG C^TCGAG
PflFI 327 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3449 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3107 GCTCTTC GCTCTTC(1/4)
SfiI 2595 GGCCCTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1852 TTCGAA TT^CGAA
SnaBI 3657 TACGTA TAC^GTA
StuI 3367 AGGCCT AGG^CCT
Tth111I 327 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3449 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2647 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 413 CCGC CCGC(-3/-1)
2379 GCGG
AlwI 7 GATCC GGATC(4/5)
2888 GATCC
AlwNI 1621 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4159 CAGGTCCTG
BclI 18 TGATCA T^GATCA
392 TGATCA
BglII 1984 AGATCT A^GATCT
2620 AGATCT
BlpI 845 GCTTAGC GC^T[ACGT]AGC
3854 GCTCAGC
Bpu1102I 845 GCTTAGC GC^T[ACGT]AGC
3854 GCTCAGC
Bsp1407I 199 TGTACA T^GTACA
3520 TGTACA
BspHI 395 TCATGA T^CATGA
1377 TCATGA
BsrDI 1146 CATTGC GCAATG(2/0)
2518 GCAATG
BsrGI 199 TGTACA T^GTACA
3520 TGTACA
BssSI 3450 CACGAG CACGAG(-5/-1)
3865 CACGAG
BstAPI 1886 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4158 GCAGGTCCTGC
BstXI 2992 CCAAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3724 CCACAGCCATGG
CelII 845 GCTTAGC GC^T[ACGT]AGC
3854 GCTCAGC
FauI 412 CCCGC CCCGC(4/6)
2379 GCGGG
FbaI 18 TGATCA T^GATCA
392 TGATCA
PstI 1618 CTGCAG CTGCA^G
2553 CTGCAG
RcaI 395 TCATGA T^CATGA
1377 TCATGA
ScaI 637 AGTACT AGT^ACT
1563 AGTACT
SspBI 199 TGTACA T^GTACA
3520 TGTACA
SspI 1208 AATATT AAT^ATT
2756 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AcsI AfaI AflII AluI ApoI
AseI AsnI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI