##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI Aor51HI ApaI AscI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbsI BcgI BlnI BpuAI
BsaHI BsaWI BseAI BseRI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI Eco47III Eco81I
EcoNI EcoRV FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
MluI MluNI MroI MscI NarI
NdeI NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 863 GGTACC G^GTACC
AccI 1430 GTCTAC GT^[AC][GT]AC
AflII 2113 CTTAAG C^TTAAG
AflIII 3347 ACATGT A^C[GA][CT]GT
AhdI 295 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 863 GGTACC G^GTACC
AspEI 295 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2866 GGATCC G^GATCC
BanI 863 GGTACC G^G[CT][GA]CC
BciVI 3481 GGATAC GTATCC(6/5)
BcnI 48 CCGGG CC^[GC]GG
BfrI 2113 CTTAAG C^TTAAG
BglI 1053 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1853 CACGTA [CT]AC^GT[GA]
BsiEI 95 CGGCCG CG[GA][CT]^CG
BspLU11I 3347 ACATGT A^CATGT
BspMI 179 ACCTGC ACCTGC(4/8)
BsrFI 97 GCCGGC [GA]^CCGG[CT]
BssHII 1333 GCGCGC G^CGCGC
BstEII 2901 GGTAACC G^GT[ACGT]ACC
BstPI 2901 GGTAACC G^GT[ACGT]ACC
Cfr10I 97 GCCGGC [GA]^CCGG[CT]
DraIII 1747 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 95 CGGCCG C^GGCCG
Eam1105I 295 GACACTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoO65I 2901 GGTAACC G^GT[ACGT]ACC
EcoT22I 2922 ATGCAT ATGCA^T
HgaI 65 GACGC GACGC(5/10)
KpnI 863 GGTACC GGTAC^C
KspI 5 CCGCGG CCGC^GG
MfeI 978 CAATTG C^AATTG
MunI 978 CAATTG C^AATTG
NaeI 97 GCCGGC GCC^GGC
NciI 48 CCGGG CC^[GC]GG
NcoI 3280 CCATGG C^CATGG
NgoMIV 97 GCCGGC G^CCGGC
NsiI 2922 ATGCAT ATGCA^T
PacI 3553 TTAATTAA TTAAT^TAA
PflMI 747 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 988 CAGCTG CAG^CTG
SacI 2588 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SpeI 2992 ACTAGT A^CTAGT
SphI 2075 GCATGC GCATG^C
SwaI 2399 ATTTAAAT ATTT^AAAT
Van91I 747 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3738 TCTAGA T^CTAGA
XcmI 4091 CCATTAAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 6 CGCG CG^CG
1334 CGCG
Alw44I 1114 GTGCAC G^TGCAC
2452 GTGCAC
ApaLI 1114 GTGCAC G^TGCAC
2452 GTGCAC
Asp700I 153 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2470 GAAATGCTTC
BanII 1412 GGGCTC G[GA]GC[CT]^C
2588 GAGCTC
BglII 194 AGATCT A^GATCT
2744 AGATCT
BsaBI 2921 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4270 GATGACAATC
BsgI 21 CTGCAC GTGCAG(16/14)
1962 GTGCAG
BsmI 3914 GAATGC GAATGC(1/-1)
1928 GCATTC
BspHI 4528 TCATGA T^CATGA
4735 TCATGA
BstUI 6 CGCG CG^CG
1334 CGCG
CfoI 1333 GCGC GCG^C
1335 GCGC
DrdI 65 GACGCACGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
672 GACCTTACTGTC
EarI 277 GAAGAG CTCTTC(1/4)
2146 GAAGAG
EcoRI 396 GAATTC G^AATTC
2966 GAATTC
HapII 48 CCGG C^CGG
98 CCGG
HhaI 1333 GCGC GCG^C
1335 GCGC
HinP1I 1333 GCGC G^CGC
1335 GCGC
HpaII 48 CCGG C^CGG
98 CCGG
Ksp632I 277 GAAGAG CTCTTC(1/4)
2146 GAAGAG
MaeII 1854 ACGT A^CGT
2177 ACGT
MamI 2921 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4270 GATGACAATC
MspI 48 CCGG C^CGG
98 CCGG
MvnI 6 CGCG CG^CG
1334 CGCG
PstI 945 CTGCAG CTGCA^G
4315 CTGCAG
RcaI 4528 TCATGA T^CATGA
4735 TCATGA
ScaI 792 AGTACT AGT^ACT
4386 AGTACT
XmnI 153 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2470 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BbvCI BbvI BclI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII