##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BcgI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MroI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflII 148 CTTAAG C^TTAAG
AocI 777 CCTTAGG CC^T[ACGT]AGG
AseI 4382 ATTAAT AT^TAAT
AsnI 4382 ATTAAT AT^TAAT
BalI 2773 TGGCCA TGG^CCA
BanI 634 GGCACC G^G[CT][GA]CC
BbvCI 4629 GCTGAGG CCTCAGC(-5/-2)
BcnI 4452 CCGGG CC^[GC]GG
BfrI 148 CTTAAG C^TTAAG
BpmI 4704 CTCCAG CTGGAG(16/14)
BsiWI 4599 CGTACG C^GTACG
BsmBI 4732 CGTCTC CGTCTC(1/5)
BspHI 665 TCATGA T^CATGA
BsrDI 993 GCAATG GCAATG(2/0)
BstEII 2127 GGTAACC G^GT[ACGT]ACC
BstPI 2127 GGTAACC G^GT[ACGT]ACC
Bsu36I 777 CCTTAGG CC^T[ACGT]AGG
CfoI 501 GCGC GCG^C
EaeI 2773 TGGCCA [CT]^GGCC[GA]
EarI 3671 CTCTTC CTCTTC(1/4)
Eco81I 777 CCTTAGG CC^T[ACGT]AGG
EcoNI 773 CCTCCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2127 GGTAACC G^GT[ACGT]ACC
EcoT14I 255 CCATGG C^C[AT][AT]GG
FauI 4501 GCGGG CCCGC(4/6)
HaeII 500 AGCGCC [GA]GCGC^[CT]
HapII 4452 CCGG C^CGG
HhaI 501 GCGC GCG^C
HinP1I 501 GCGC G^CGC
HincII 1676 GTTGAC GT[CT]^[GA]AC
HindII 1676 GTTGAC GT[CT]^[GA]AC
HpaII 4452 CCGG C^CGG
Ksp632I 3671 CTCTTC CTCTTC(1/4)
MfeI 109 CAATTG C^AATTG
MluI 4588 ACGCGT A^CGCGT
MluNI 2773 TGGCCA TGG^CCA
MscI 2773 TGGCCA TGG^CCA
MspI 4452 CCGG C^CGG
MunI 109 CAATTG C^AATTG
NciI 4452 CCGGG CC^[GC]GG
NcoI 255 CCATGG C^CATGG
NdeI 3729 CATATG CA^TATG
NheI 2315 GCTAGC G^CTAGC
NlaIV 634 GGCACC GG[ACGT]^[ACGT]CC
PshAI 3646 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4382 ATTAAT AT^TAAT
PstI 300 CTGCAG CTGCA^G
RcaI 665 TCATGA T^CATGA
SexAI 4018 ACCAGGT A^CC[AT]GGT
SmlI 148 CTTAAG C^T[CT][GA]AG
StyI 255 CCATGG C^C[AT][AT]GG
TaqI 201 TCGA T^CGA
TthHB8I 201 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 4589 CGCG CG^CG
4692 CGCG
AclI 3559 AACGTT AA^CGTT
3825 AACGTT
AspI 3850 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4727 GACTCCGTC
BbsI 1574 GTCTTC GAAGAC(2/6)
3184 GTCTTC
BclI 2740 TGATCA T^GATCA
3437 TGATCA
BglII 3337 AGATCT A^GATCT
4342 AGATCT
BmyI 633 GGGCAC G[AGT]GC[ACT]^C
4454 GGGCAC
BpuAI 1574 GTCTTC GAAGAC(2/6)
3184 GTCTTC
BsaI 4528 GAGACC GGTCTC(1/5)
3855 GGTCTC
BsmFI 413 GGGAC GGGAC(10/14)
4611 GTCCC
Bsp1286I 633 GGGCAC G[AGT]GC[ACT]^C
4454 GGGCAC
Bst1107I 3147 GTATAC GTA^TAC
4208 GTATAC
BstUI 4589 CGCG CG^CG
4692 CGCG
BstXI 2550 CCAATTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4614 CCAGCTACTTGG
BstZ17I 3147 GTATAC GTA^TAC
4208 GTATAC
DraII 2796 AGGACCT [GA]G^G[ACGT]CC[CT]
2975 AGGACCT
DsaI 255 CCATGG C^C[GA][CT]GG
638 CCGTGG
EcoO109I 2796 AGGACCT [GA]G^G[ACGT]CC[CT]
2975 AGGACCT
EcoRI 70 GAATTC G^AATTC
310 GAATTC
EcoT22I 1092 ATGCAT ATGCA^T
3712 ATGCAT
FbaI 2740 TGATCA T^GATCA
3437 TGATCA
HgaI 140 GACGC GACGC(5/10)
4693 GCGTC
MvnI 4589 CGCG CG^CG
4692 CGCG
NsiI 1092 ATGCAT ATGCA^T
3712 ATGCAT
PflFI 3850 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4727 GACTCCGTC
PpuMI 2796 AGGACCT [GA]G^G[AT]CC[CT]
2975 AGGACCT
Psp1406I 3559 AACGTT AA^CGTT
3825 AACGTT
SphI 143 GCATGC GCATG^C
834 GCATGC
Tth111I 3850 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4727 GACTCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AvaII
BbvI BciVI BfaI BsaJI BsgI
BsiYI BslI BsmAI BsmI Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI