##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvrII BamHI
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BspDI BspLU11I BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 1945 CGCG CG^CG
AccIII 1967 TCCGGA T^CCGGA
AclI 2013 AACGTT AA^CGTT
AocI 1237 CCTGAGG CC^T[ACGT]AGG
Asp700I 3936 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 133 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4064 CCCGGG C^[CT]CG[GA]G
AviII 1980 TGCGCA TGC^GCA
BanII 1420 GGGCTC G[GA]GC[CT]^C
BclI 330 TGATCA T^GATCA
BglI 1597 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3279 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3279 GCTGAGC GC^T[ACGT]AGC
BsaI 2443 GGTCTC GGTCTC(1/5)
BseAI 1967 TCCGGA T^CCGGA
BsoBI 4064 CCCGGG C^[CT]CG[GA]G
BspEI 1967 TCCGGA T^CCGGA
BspMI 970 GCAGGT ACCTGC(4/8)
BsrBI 876 CCGCTC CCGCTC(-3/-3)
BssSI 839 CTCGTG CACGAG(-5/-1)
BstUI 1945 CGCG CG^CG
Bsu36I 1237 CCTGAGG CC^T[ACGT]AGG
CelII 3279 GCTGAGC GC^T[ACGT]AGC
DraIII 467 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1237 CCTGAGG CC^T[ACGT]AGG
EcoNI 4036 CCTAAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3947 GAATTC G^AATTC
FbaI 330 TGATCA T^GATCA
FspI 1980 TGCGCA TGC^GCA
HincII 3441 GTCAAC GT[CT]^[GA]AC
HindII 3441 GTCAAC GT[CT]^[GA]AC
MfeI 581 CAATTG C^AATTG
MroI 1967 TCCGGA T^CCGGA
MunI 581 CAATTG C^AATTG
MvnI 1945 CGCG CG^CG
NdeI 3863 CATATG CA^TATG
PflFI 133 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1858 GACACTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2013 AACGTT AA^CGTT
SapI 2296 GCTCTTC GCTCTTC(1/4)
SmaI 4064 CCCGGG CCC^GGG
SpeI 1861 ACTAGT A^CTAGT
SspI 19 AATATT AAT^ATT
StuI 1142 AGGCCT AGG^CCT
Tth111I 133 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4064 CCCGGG C^CCGGG
XmaI 4064 CCCGGG C^CCGGG
XmnI 3936 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanI 871 GGTGCC G^G[CT][GA]CC
1118 GGCACC
BsaAI 1951 CACGTA [CT]AC^GT[GA]
1984 CACGTA
BsaBI 715 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3169 GATGCCAATC
BsaWI 1329 TCCGGT [AT]^CCGG[AT]
1967 TCCGGA
BsmFI 1911 GTCCC GGGAC(10/14)
1941 GTCCC
Bsp1407I 1086 TGTACA T^GTACA
2053 TGTACA
BspHI 1916 TCATGA T^CATGA
3993 TCATGA
BsrGI 1086 TGTACA T^GTACA
2053 TGTACA
BstAPI 589 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3508 GCAGTGGTTGC
BstEII 1685 GGTTACC G^GT[ACGT]ACC
2766 GGTGACC
BstPI 1685 GGTTACC G^GT[ACGT]ACC
2766 GGTGACC
DraI 529 TTTAAA TTT^AAA
2950 TTTAAA
EcoO65I 1685 GGTTACC G^GT[ACGT]ACC
2766 GGTGACC
EcoRV 2861 GATATC GAT^ATC
2871 GATATC
MamI 715 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3169 GATGCCAATC
RcaI 1916 TCATGA T^CATGA
3993 TCATGA
SspBI 1086 TGTACA T^GTACA
2053 TGTACA
TaqI 1164 TCGA T^CGA
2118 TCGA
TthHB8I 1164 TCGA T^CGA
2118 TCGA
XbaI 1384 TCTAGA T^CTAGA
3117 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII