##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I ApaLI AscI AspI AvaII
AviII AvrII BbeI BbrPI BbvCI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI DsaI EagI
EclXI Eco52I EcoO65I EcoT14I FauI
FseI FspI Hin1I HpaI KasI
KspI MluI MroI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 269 GGTACC G^GTACC
AccII 932 CGCG CG^CG
AhdI 2205 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2603 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 680 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3392 AGCGCT AGC^GCT
Asp718I 269 GGTACC G^GTACC
AspEI 2205 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1772 TGGCCA TGG^CCA
BcgI 2527 GCAACACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 945 GGATAC GTATCC(6/5)
BclI 1577 TGATCA T^GATCA
BsaBI 519 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1960 GAGACC GGTCTC(1/5)
BsaWI 949 ACCGGA [AT]^CCGG[AT]
BstAPI 737 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 932 CGCG CG^CG
Bsu36I 680 CCTGAGG CC^T[ACGT]AGG
Eam1105I 2205 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3392 AGCGCT AGC^GCT
Eco81I 680 CCTGAGG CC^T[ACGT]AGG
FbaI 1577 TGATCA T^GATCA
HaeII 3392 AGCGCT [GA]GCGC^[CT]
KpnI 269 GGTACC GGTAC^C
MamI 519 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1772 TGGCCA TGG^CCA
MscI 1772 TGGCCA TGG^CCA
MspA1I 2083 CAGCTG C[AC]G^C[GT]G
MvnI 932 CGCG CG^CG
NaeI 812 GCCGGC GCC^GGC
NgoMIV 812 GCCGGC G^CCGGC
PflMI 256 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2083 CAGCTG CAG^CTG
SapI 1939 GCTCTTC GCTCTTC(1/4)
Van91I 256 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1212 GTAGAC GT^[AC][GT]AC
1524 GTAGAC
AflII 1110 CTTAAG C^TTAAG
1871 CTTAAG
AflIII 1116 ACATGT A^C[GA][CT]GT
3101 ACATGT
ApaI 1799 GGGCCC GGGCC^C
2342 GGGCCC
AseI 1718 ATTAAT AT^TAAT
2705 ATTAAT
AsnI 1718 ATTAAT AT^TAAT
2705 ATTAAT
BamHI 401 GGATCC G^GATCC
893 GGATCC
BfrI 1110 CTTAAG C^TTAAG
1871 CTTAAG
BpmI 308 CTCCAG CTGGAG(16/14)
475 CTGGAG
BspHI 73 TCATGA T^CATGA
2951 TCATGA
BspLU11I 1116 ACATGT A^CATGT
3101 ACATGT
BspMI 326 ACCTGC ACCTGC(4/8)
1454 ACCTGC
BsrFI 812 GCCGGC [GA]^CCGG[CT]
1047 ACCGGC
CfoI 2129 GCGC GCG^C
3393 GCGC
Cfr10I 812 GCCGGC [GA]^CCGG[CT]
1047 ACCGGC
DraII 1799 GGGCCCT [GA]G^G[ACGT]CC[CT]
2342 GGGCCCT
EaeI 1049 CGGCCA [CT]^GGCC[GA]
1772 TGGCCA
EcoO109I 1799 GGGCCCT [GA]G^G[ACGT]CC[CT]
2342 GGGCCCT
EcoRV 1017 GATATC GAT^ATC
3885 GATATC
EcoT22I 3408 ATGCAT ATGCA^T
3695 ATGCAT
HgaI 1008 GACGC GACGC(5/10)
2075 GCGTC
HhaI 2129 GCGC GCG^C
3393 GCGC
HinP1I 2129 GCGC G^CGC
3393 GCGC
HincII 3366 GTCAAC GT[CT]^[GA]AC
3506 GTTGAC
HindII 3366 GTCAAC GT[CT]^[GA]AC
3506 GTTGAC
NsiI 3408 ATGCAT ATGCA^T
3695 ATGCAT
PleI 2303 GACTC GAGTC(4/5)
2674 GAGTC
PshBI 1718 ATTAAT AT^TAAT
2705 ATTAAT
PstI 2386 CTGCAG CTGCA^G
3957 CTGCAG
RcaI 73 TCATGA T^CATGA
2951 TCATGA
SacI 305 GAGCTC GAGCT^C
3355 GAGCTC
SexAI 1391 ACCAGGT A^CC[AT]GGT
2601 ACCAGGT
SmaI 1754 CCCGGG CCC^GGG
2044 CCCGGG
SphI 1967 GCATGC GCATG^C
3808 GCATGC
Tsp45I 166 GTGAC ^GT[GC]AC
3950 GTGAC
XcmI 256 CCACCACTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
324 CCACCTGCCTACTGG
XmaCI 1754 CCCGGG C^CCGGG
2044 CCCGGG
XmaI 1754 CCCGGG C^CCGGG
2044 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoNI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI