##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BanI BanII
BbeI BbsI BbvCI BciVI BcnI
BlnI BpuAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI BstXI CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MluI MroI MspA1I
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 397 GTATAC GT^[AC][GT]AC
AciI 1626 CCGC CCGC(-3/-1)
AflII 144 CTTAAG C^TTAAG
AocI 2301 CCTAAGG CC^T[ACGT]AGG
AspHI 3577 GTGCTC G[AT]GC[AT]^C
BbrPI 973 CACGTG CAC^GTG
BcgI 2582 CGAGGTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 144 CTTAAG C^TTAAG
BglI 1249 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1461 AGATCT A^GATCT
BlpI 3579 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3579 GCTCAGC GC^T[ACGT]AGC
BsaAI 973 CACGTG [CT]AC^GT[GA]
BsaBI 4129 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3577 GTGCTC G[AT]GC[AT]^C
BsmI 1591 GAATGC GAATGC(1/-1)
BspHI 1620 TCATGA T^CATGA
BspLU11I 3804 ACATGT A^CATGT
BspMI 2838 GCAGGT ACCTGC(4/8)
Bst1107I 397 GTATAC GTA^TAC
BstBI 290 TTCGAA TT^CGAA
BstZ17I 397 GTATAC GTA^TAC
Bsu36I 2301 CCTAAGG CC^T[ACGT]AGG
CelII 3579 GCTCAGC GC^T[ACGT]AGC
Eco81I 2301 CCTAAGG CC^T[ACGT]AGG
EcoT14I 1612 CCATGG C^C[AT][AT]GG
MamI 4129 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1719 CAATTG C^AATTG
MunI 1719 CAATTG C^AATTG
NcoI 1612 CCATGG C^CATGG
NheI 487 GCTAGC G^CTAGC
NspV 290 TTCGAA TT^CGAA
PflMI 3615 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 973 CACGTG CAC^GTG
PmlI 973 CACGTG CAC^GTG
RcaI 1620 TCATGA T^CATGA
SapI 2821 GAAGAGC GCTCTTC(1/4)
SfcI 2815 CTATAG C^T[GA][CT]AG
SfuI 290 TTCGAA TT^CGAA
StyI 1612 CCATGG C^C[AT][AT]GG
Van91I 3615 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 262 AACGTT AA^CGTT
3882 AACGTT
AseI 90 ATTAAT AT^TAAT
1168 ATTAAT
AsnI 90 ATTAAT AT^TAAT
1168 ATTAAT
Asp700I 460 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1447 GAATATTTTC
BalI 1436 TGGCCA TGG^CCA
3150 TGGCCA
BamHI 1401 GGATCC G^GATCC
2342 GGATCC
BclI 969 TGATCA T^GATCA
2853 TGATCA
BmyI 3088 GTGCCC G[AGT]GC[ACT]^C
3577 GTGCTC
BpmI 2355 CTGGAG CTGGAG(16/14)
3713 CTGGAG
BsmFI 516 GGGAC GGGAC(10/14)
1830 GGGAC
Bsp1286I 3088 GTGCCC G[AGT]GC[ACT]^C
3577 GTGCTC
DraIII 908 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3616 CACCTGGTG
DsaI 1612 CCATGG C^C[GA][CT]GG
3651 CCGTGG
EaeI 1436 TGGCCA [CT]^GGCC[GA]
3150 TGGCCA
EcoRI 2491 GAATTC G^AATTC
2926 GAATTC
EcoRV 302 GATATC GAT^ATC
882 GATATC
FbaI 969 TGATCA T^GATCA
2853 TGATCA
HphI 213 GGTGA GGTGA(8/7)
3225 GGTGA
MluNI 1436 TGGCCA TGG^CCA
3150 TGGCCA
MscI 1436 TGGCCA TGG^CCA
3150 TGGCCA
PshBI 90 ATTAAT AT^TAAT
1168 ATTAAT
Psp1406I 262 AACGTT AA^CGTT
3882 AACGTT
SexAI 3444 ACCTGGT A^CC[AT]GGT
3617 ACCTGGT
Tsp45I 914 GTGAC ^GT[GC]AC
1119 GTCAC
XcmI 1840 CCAGAAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3642 CCATCTGAACCGTGG
XmnI 460 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1447 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI AvaII BbvI BfaI
BsaJI BseRI BsiYI BslI BsmAI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SmlI SspI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII