##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AspEI AvaI AviII
AvrII BalI BamHI BanII BbeI
BcgI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KspI MflI MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3753 GGTACC G^GTACC
AflII 2199 CTTAAG C^TTAAG
AlwI 3150 GGATC GGATC(4/5)
Asp718I 3753 GGTACC G^GTACC
AspI 3480 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3433 CACGTG CAC^GTG
BbvCI 3644 GCTGAGG CCTCAGC(-5/-2)
BfrI 2199 CTTAAG C^TTAAG
BsaAI 3433 CACGTG [CT]AC^GT[GA]
BsaBI 3775 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1979 GAGACC GGTCTC(1/5)
Bsp1407I 2124 TGTACA T^GTACA
BsrBI 881 GAGCGG CCGCTC(-3/-3)
BsrGI 2124 TGTACA T^GTACA
Bst1107I 2655 GTATAC GTA^TAC
BstAPI 1846 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2655 GTATAC GTA^TAC
DsaI 1759 CCATGG C^C[GA][CT]GG
EcoRI 3192 GAATTC G^AATTC
EcoT22I 2986 ATGCAT ATGCA^T
FauI 2010 CCCGC CCCGC(4/6)
HgaI 2152 GACGC GACGC(5/10)
KpnI 3753 GGTACC GGTAC^C
MamI 3775 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2945 CAATTG C^AATTG
MspA1I 532 CAGCTG C[AC]G^C[GT]G
MunI 2945 CAATTG C^AATTG
NcoI 1759 CCATGG C^CATGG
NsiI 2986 ATGCAT ATGCA^T
PacI 2616 TTAATTAA TTAAT^TAA
PflFI 3480 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3433 CACGTG CAC^GTG
PmlI 3433 CACGTG CAC^GTG
PstI 3253 CTGCAG CTGCA^G
PvuII 532 CAGCTG CAG^CTG
ScaI 3297 AGTACT AGT^ACT
SfcI 3253 CTGCAG C^T[GA][CT]AG
SphI 2988 GCATGC GCATG^C
SspBI 2124 TGTACA T^GTACA
SwaI 3909 ATTTAAAT ATTT^AAAT
Tsp45I 3394 GTGAC ^GT[GC]AC
Tth111I 3480 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 943 CCAAAAATCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Alw44I 3430 GTGCAC G^TGCAC
3845 GTGCAC
ApaLI 3430 GTGCAC G^TGCAC
3845 GTGCAC
AspHI 3430 GTGCAC G[AT]GC[AT]^C
3845 GTGCAC
AvaII 2488 GGTCC G^G[AT]CC
2603 GGACC
BanI 2430 GGCACC G^G[CT][GA]CC
3753 GGTACC
BbvI 531 GCAGC GCAGC(8/12)
2846 GCTGC
BpmI 3488 CTCCAG CTGGAG(16/14)
3047 CTGGAG
BsiHKAI 3430 GTGCAC G[AT]GC[AT]^C
3845 GTGCAC
BsrDI 297 CATTGC GCAATG(2/0)
1252 CATTGC
MaeII 1555 ACGT A^CGT
3434 ACGT
PflMI 2420 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2971 CCATAAAATGG
SapI 878 GAAGAGC GCTCTTC(1/4)
1383 GAAGAGC
SmlI 1733 CTTGAG C^T[CT][GA]AG
2199 CTTAAG
TseI 531 GCAGC G^C[AT]GC
2846 GCTGC
Van91I 2420 CCATCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2971 CCATAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI Asp700I
BbsI BciVI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BspHI BspLU11I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SspI StyI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I XmnI