##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FbaI
FseI FspI Hin1I KasI KpnI
KspI MluI MvnI NarI NciI
NcoI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmlI
PshAI PshBI PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 963 TCCGGA T^CCGGA
AclI 2387 AACGTT AA^CGTT
AspI 1134 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2843 CCCGAG C^[CT]CG[GA]G
BalI 1672 TGGCCA TGG^CCA
BbvCI 1285 CCTCAGC CCTCAGC(-5/-2)
BglII 1320 AGATCT A^GATCT
BsaAI 3404 CACGTA [CT]AC^GT[GA]
BsaWI 963 TCCGGA [AT]^CCGG[AT]
BseAI 963 TCCGGA T^CCGGA
BsoBI 2843 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3765 TGTACA T^GTACA
BspEI 963 TCCGGA T^CCGGA
BspMI 1218 ACCTGC ACCTGC(4/8)
BsrBI 1314 CCGCTC CCGCTC(-3/-3)
BsrFI 1526 GCCGGC [GA]^CCGG[CT]
BsrGI 3765 TGTACA T^GTACA
BstXI 347 CCATGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 776 GCGC GCG^C
Cfr10I 1526 GCCGGC [GA]^CCGG[CT]
DraII 382 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1344 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 382 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1555 GAATTC G^AATTC
EcoRV 2495 GATATC GAT^ATC
HaeII 775 AGCGCC [GA]GCGC^[CT]
HgaI 1290 GCGTC GACGC(5/10)
HhaI 776 GCGC GCG^C
HinP1I 776 GCGC G^CGC
HpaI 3743 GTTAAC GTT^AAC
MluNI 1672 TGGCCA TGG^CCA
MroI 963 TCCGGA T^CCGGA
MscI 1672 TGGCCA TGG^CCA
NaeI 1526 GCCGGC GCC^GGC
NgoMIV 1526 GCCGGC G^CCGGC
PflFI 1134 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 985 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 2135 GTTTAAAC GTTT^AAAC
PpuMI 382 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2387 AACGTT AA^CGTT
PvuII 1357 CAGCTG CAG^CTG
ScaI 2604 AGTACT AGT^ACT
SphI 3551 GCATGC GCATG^C
SspBI 3765 TGTACA T^GTACA
SspI 80 AATATT AAT^ATT
Tth111I 1134 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 985 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2906 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 3015 CTTAAG C^TTAAG
3418 CTTAAG
AocI 1327 CCTCAGG CC^T[ACGT]AGG
3209 CCTAAGG
BbsI 2925 GAAGAC GAAGAC(2/6)
1056 GTCTTC
BfrI 3015 CTTAAG C^TTAAG
3418 CTTAAG
BpuAI 2925 GAAGAC GAAGAC(2/6)
1056 GTCTTC
BsaBI 1144 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2807 GATACAAATC
BsaI 2331 GAGACC GGTCTC(1/5)
1248 GGTCTC
BsmI 543 GAATGC GAATGC(1/-1)
3227 GCATTC
BspLU11I 1075 ACATGT A^CATGT
3726 ACATGT
BsrDI 2981 CATTGC GCAATG(2/0)
2199 GCAATG
Bsu36I 1327 CCTCAGG CC^T[ACGT]AGG
3209 CCTAAGG
EaeI 1528 CGGCCA [CT]^GGCC[GA]
1672 TGGCCA
Eco81I 1327 CCTCAGG CC^T[ACGT]AGG
3209 CCTAAGG
EcoT22I 2169 ATGCAT ATGCA^T
3553 ATGCAT
FauI 767 CCCGC CCCGC(4/6)
753 GCGGG
HapII 964 CCGG C^CGG
1527 CCGG
HindIII 2246 AAGCTT A^AGCTT
2435 AAGCTT
HpaII 964 CCGG C^CGG
1527 CCGG
MamI 1144 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2807 GATACAAATC
MfeI 2934 CAATTG C^AATTG
3062 CAATTG
MspA1I 751 CAGCGG C[AC]G^C[GT]G
1357 CAGCTG
MspI 964 CCGG C^CGG
1527 CCGG
MunI 2934 CAATTG C^AATTG
3062 CAATTG
NdeI 2172 CATATG CA^TATG
2689 CATATG
NsiI 2169 ATGCAT ATGCA^T
3553 ATGCAT
StuI 458 AGGCCT AGG^CCT
3900 AGGCCT
SwaI 2757 ATTTAAAT ATTT^AAAT
2942 ATTTAAAT
TaqI 180 TCGA T^CGA
1833 TCGA
TthHB8I 180 TCGA T^CGA
1833 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraIII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI