##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI BamHI BbeI BbrPI
BcgI BcnI BglI BglII BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstYI CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FauI FseI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KspI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1016 GGTACC G^GTACC
AflII 56 CTTAAG C^TTAAG
AlwI 1478 GGATC GGATC(4/5)
Asp700I 1991 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1016 GGTACC G^GTACC
AviII 2510 TGCGCA TGC^GCA
AvrII 1628 CCTAGG C^CTAGG
BalI 976 TGGCCA TGG^CCA
BanI 1016 GGTACC G^G[CT][GA]CC
BbvCI 1293 GCTGAGG CCTCAGC(-5/-2)
BciVI 3038 GGATAC GTATCC(6/5)
BclI 2384 TGATCA T^GATCA
BfrI 56 CTTAAG C^TTAAG
BlnI 1628 CCTAGG C^CTAGG
BsaBI 2683 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1408 GGTCTC GGTCTC(1/5)
BsmBI 920 GAGACG CGTCTC(1/5)
BsmFI 1660 GGGAC GGGAC(10/14)
BsmI 2513 GCATTC GAATGC(1/-1)
BspHI 44 TCATGA T^CATGA
BspMI 420 ACCTGC ACCTGC(4/8)
Bst1107I 942 GTATAC GTA^TAC
BstXI 691 CCAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 942 GTATAC GTA^TAC
CfoI 2511 GCGC GCG^C
EaeI 976 TGGCCA [CT]^GGCC[GA]
EarI 2824 CTCTTC CTCTTC(1/4)
FbaI 2384 TGATCA T^GATCA
FspI 2510 TGCGCA TGC^GCA
HapII 586 CCGG C^CGG
HhaI 2511 GCGC GCG^C
HinP1I 2511 GCGC G^CGC
HpaII 586 CCGG C^CGG
KpnI 1016 GGTACC GGTAC^C
Ksp632I 2824 CTCTTC CTCTTC(1/4)
MaeII 923 ACGT A^CGT
MamI 2683 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 976 TGGCCA TGG^CCA
MscI 976 TGGCCA TGG^CCA
MspI 586 CCGG C^CGG
NdeI 2878 CATATG CA^TATG
NspI 661 GCATGC [GA]CATG^[CT]
PstI 739 CTGCAG CTGCA^G
RcaI 44 TCATGA T^CATGA
SacI 1849 GAGCTC GAGCT^C
SexAI 1012 ACCTGGT A^CC[AT]GGT
SphI 661 GCATGC GCATG^C
StuI 2366 AGGCCT AGG^CCT
XmnI 1991 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 954 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1008 CAGGACCTG
AocI 818 CCTGAGG CC^T[ACGT]AGG
2276 CCTTAGG
AvaI 1446 CCCGAG C^[CT]CG[GA]G
2362 CCCGAG
AvaII 1010 GGACC G^G[AT]CC
1661 GGACC
BsoBI 1446 CCCGAG C^[CT]CG[GA]G
2362 CCCGAG
Bsp1407I 1191 TGTACA T^GTACA
1888 TGTACA
BsrGI 1191 TGTACA T^GTACA
1888 TGTACA
Bsu36I 818 CCTGAGG CC^T[ACGT]AGG
2276 CCTTAGG
DraII 1009 AGGACCT [GA]G^G[ACGT]CC[CT]
1660 GGGACCT
Eco81I 818 CCTGAGG CC^T[ACGT]AGG
2276 CCTTAGG
EcoO109I 1009 AGGACCT [GA]G^G[ACGT]CC[CT]
1660 GGGACCT
EcoRV 703 GATATC GAT^ATC
1098 GATATC
PflMI 425 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
979 CCAAGGAATGG
PpuMI 1009 AGGACCT [GA]G^G[AT]CC[CT]
1660 GGGACCT
PvuII 157 CAGCTG CAG^CTG
1696 CAGCTG
ScaI 1076 AGTACT AGT^ACT
2913 AGTACT
SmlI 56 CTTAAG C^T[CT][GA]AG
1858 CTCAAG
SspBI 1191 TGTACA T^GTACA
1888 TGTACA
SspI 2611 AATATT AAT^ATT
2843 AATATT
Van91I 425 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
979 CCAAGGAATGG
XbaI 2059 TCTAGA T^CTAGA
2194 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AspHI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I