##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AlwNI Aor51HI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BanI BbeI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsiEI
BsiWI BsmBI BspDI BspHI BspMI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DsaI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
FseI FspI HaeII HpaI KasI
KpnI KspI MamI MfeI MluI
MspA1I MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI StyI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2491 GTAGAC GT^[AC][GT]AC
AccII 1056 CGCG CG^CG
AccIII 992 TCCGGA T^CCGGA
AcyI 247 GGCGTC G[GA]^CG[CT]C
AflIII 1269 ACATGT A^C[GA][CT]GT
AhdI 1059 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1429 GGGCCC GGGCC^C
AspEI 1059 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 514 CCCGAG C^[CT]CG[GA]G
BalI 2115 TGGCCA TGG^CCA
BbrPI 850 CACGTG CAC^GTG
BciVI 1025 GGATAC GTATCC(6/5)
BclI 177 TGATCA T^GATCA
BglI 166 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 850 CACGTG [CT]AC^GT[GA]
BsaHI 247 GGCGTC G[GA]^CG[CT]C
BseAI 992 TCCGGA T^CCGGA
BsmFI 2678 GTCCC GGGAC(10/14)
BsoBI 514 CCCGAG C^[CT]CG[GA]G
BspEI 992 TCCGGA T^CCGGA
BspLU11I 1269 ACATGT A^CATGT
BsrDI 3038 GCAATG GCAATG(2/0)
BssSI 741 CACGAG CACGAG(-5/-1)
BstAPI 2237 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1056 CGCG CG^CG
BstYI 323 AGATCC [GA]^GATC[CT]
DraII 1429 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1280 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 241 GACAGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1059 GACTTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 737 CCTTCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1429 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 1232 GCGGG CCCGC(4/6)
FbaI 177 TGATCA T^GATCA
HgaI 248 GCGTC GACGC(5/10)
Hin1I 247 GGCGTC G[GA]^CG[CT]C
HincII 281 GTCAAC GT[CT]^[GA]AC
HindII 281 GTCAAC GT[CT]^[GA]AC
MflI 323 AGATCC [GA]^GATC[CT]
MluNI 2115 TGGCCA TGG^CCA
MroI 992 TCCGGA T^CCGGA
MscI 2115 TGGCCA TGG^CCA
MvnI 1056 CGCG CG^CG
NdeI 962 CATATG CA^TATG
PflMI 1420 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 850 CACGTG CAC^GTG
PmlI 850 CACGTG CAC^GTG
PstI 614 CTGCAG CTGCA^G
TfiI 669 GAATC G^A[AT]TC
Van91I 1420 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2503 TCTAGA T^CTAGA
XhoII 323 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 1015 GTGCAC G^TGCAC
1997 GTGCAC
AlwI 324 GATCC GGATC(4/5)
2082 GGATC
ApaLI 1015 GTGCAC G^TGCAC
1997 GTGCAC
AvaII 233 GGACC G^G[AT]CC
2379 GGTCC
BcnI 1289 CCGGG CC^[GC]GG
2022 CCCGG
BsaI 1293 GAGACC GGTCTC(1/5)
1378 GAGACC
BsgI 2268 CTGCAC GTGCAG(16/14)
266 GTGCAG
CfoI 1055 GCGC GCG^C
2262 GCGC
DraI 2877 TTTAAA TTT^AAA
2999 TTTAAA
EaeI 2115 TGGCCA [CT]^GGCC[GA]
2564 TGGCCG
EcoRI 437 GAATTC G^AATTC
2217 GAATTC
HhaI 1055 GCGC GCG^C
2262 GCGC
HinP1I 1055 GCGC G^CGC
2262 GCGC
HindIII 2010 AAGCTT A^AGCTT
2328 AAGCTT
MaeII 851 ACGT A^CGT
1013 ACGT
NciI 1289 CCGGG CC^[GC]GG
2022 CCCGG
SfcI 153 CTACAG C^T[GA][CT]AG
614 CTGCAG
SmlI 391 CTTGAG C^T[CT][GA]AG
1074 CTTGAG
SphI 1219 GCATGC GCATG^C
2174 GCATGC
TaqI 667 TCGA T^CGA
2103 TCGA
TthHB8I 667 TCGA T^CGA
2103 TCGA
XcmI 1596 CCATCTATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2016 CCATCTCCCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AocI
ApoI Asp700I AspHI BanII BbsI
BbvCI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstXI Bsu36I Cac8I Cfr13I
DdeI DpnII EarI Eco81I EcoRII
EcoT22I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SspBI SspI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI