##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
Aor51HI AscI AseI AsnI AviII
BamHI BbrPI BcgI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfuI SnaBI SphI
Sse8387I SwaI TaqI TfiI TthHB8I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2199 GGTACC G^GTACC
AccII 8 CGCG CG^CG
AclI 278 AACGTT AA^CGTT
AflIII 1793 ACATGT A^C[GA][CT]GT
AhdI 2637 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 598 GTGCAC G^TGCAC
AocI 1663 CCTGAGG CC^T[ACGT]AGG
ApaLI 598 GTGCAC G^TGCAC
Asp700I 2549 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2199 GGTACC G^GTACC
AspEI 2637 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1876 CCTAGG C^CTAGG
BalI 638 TGGCCA TGG^CCA
BbeI 1700 GGCGCC GGCGC^C
BbsI 202 GTCTTC GAAGAC(2/6)
BciVI 714 GTATCC GTATCC(6/5)
BclI 590 TGATCA T^GATCA
BlnI 1876 CCTAGG C^CTAGG
BpuAI 202 GTCTTC GAAGAC(2/6)
BsaI 25 GGTCTC GGTCTC(1/5)
BspLU11I 1793 ACATGT A^CATGT
BsrBI 1372 GAGCGG CCGCTC(-3/-3)
BsrFI 1697 GCCGGC [GA]^CCGG[CT]
BstUI 8 CGCG CG^CG
BstYI 1936 GGATCT [GA]^GATC[CT]
Bsu36I 1663 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1697 GCCGGC [GA]^CCGG[CT]
DrdI 2411 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2637 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1663 CCTGAGG CC^T[ACGT]AGG
FbaI 590 TGATCA T^GATCA
HincII 1504 GTCAAC GT[CT]^[GA]AC
HindII 1504 GTCAAC GT[CT]^[GA]AC
KasI 1700 GGCGCC G^GCGCC
KpnI 2199 GGTACC GGTAC^C
MaeII 279 ACGT A^CGT
MflI 1936 GGATCT [GA]^GATC[CT]
MluNI 638 TGGCCA TGG^CCA
MscI 638 TGGCCA TGG^CCA
MvnI 8 CGCG CG^CG
NaeI 1697 GCCGGC GCC^GGC
NarI 1700 GGCGCC GG^CGCC
NgoMIV 1697 GCCGGC G^CCGGC
PshAI 1912 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 278 AACGTT AA^CGTT
SfiI 1481 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1700 GGCGCC GGC^GCC
SgrAI 1696 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2611 CCCGGG CCC^GGG
SmlI 2402 CTCAAG C^T[CT][GA]AG
SpeI 2562 ACTAGT A^CTAGT
SspI 2780 AATATT AAT^ATT
XhoII 1936 GGATCT [GA]^GATC[CT]
XmaCI 2611 CCCGGG C^CCGGG
XmaI 2611 CCCGGG C^CCGGG
XmnI 2549 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2580 AAATTT [GA]^AATT[CT]
2790 AAATTT
AcyI 1429 GACGCC G[GA]^CG[CT]C
1700 GGCGCC
AlwI 936 GGATC GGATC(4/5)
1936 GGATC
ApaI 1053 GGGCCC GGGCC^C
1480 GGGCCC
ApoI 2580 AAATTT [GA]^AATT[CT]
2790 AAATTT
AspI 150 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
751 GACAATGTC
AvaI 1940 CTCGGG C^[CT]CG[GA]G
2611 CCCGGG
BbvCI 979 CCTCAGC CCTCAGC(-5/-2)
400 GCTGAGG
BsaHI 1429 GACGCC G[GA]^CG[CT]C
1700 GGCGCC
BsmI 2317 GAATGC GAATGC(1/-1)
2600 GAATGC
BsoBI 1940 CTCGGG C^[CT]CG[GA]G
2611 CCCGGG
BspHI 578 TCATGA T^CATGA
587 TCATGA
BsrDI 40 GCAATG GCAATG(2/0)
745 GCAATG
BstXI 629 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2634 CCAGACTCCTGG
DraIII 472 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1804 CACCAAGTG
EaeI 68 CGGCCA [CT]^GGCC[GA]
638 TGGCCA
HgaI 1429 GACGC GACGC(5/10)
2283 GCGTC
Hin1I 1429 GACGCC G[GA]^CG[CT]C
1700 GGCGCC
NcoI 1049 CCATGG C^CATGG
1930 CCATGG
PflFI 150 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
751 GACAATGTC
PpuMI 2355 GGGACCC [GA]G^G[AT]CC[CT]
2695 GGGACCT
PvuII 828 CAGCTG CAG^CTG
1157 CAGCTG
RcaI 578 TCATGA T^CATGA
587 TCATGA
StuI 188 AGGCCT AGG^CCT
1624 AGGCCT
Tth111I 150 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
751 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI