##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AclI AflIII AscI
Asp718I BbeI BciVI BglII BsaBI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoT22I HindIII
HpaI KasI KpnI KspI MamI
MluI MroI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2219 GACGTC GACGT^C
AccI 4990 GTAGAC GT^[AC][GT]AC
AcsI 3973 AAATTT [GA]^AATT[CT]
AflII 5769 CTTAAG C^TTAAG
AgeI 4961 ACCGGT A^CCGGT
AhdI 4795 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 355 AGCGCT AGC^GCT
ApoI 3973 AAATTT [GA]^AATT[CT]
AseI 3719 ATTAAT AT^TAAT
AsnI 3719 ATTAAT AT^TAAT
AspEI 4795 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1333 TGCGCA TGC^GCA
BamHI 3909 GGATCC G^GATCC
BbrPI 1103 CACGTG CAC^GTG
BclI 634 TGATCA T^GATCA
BfrI 5769 CTTAAG C^TTAAG
BsaWI 4961 ACCGGT [AT]^CCGG[AT]
BsmI 854 GAATGC GAATGC(1/-1)
BspMI 1450 ACCTGC ACCTGC(4/8)
BstAPI 2922 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 5580 GACAGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4795 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 355 AGCGCT AGC^GCT
EcoRV 668 GATATC GAT^ATC
FbaI 634 TGATCA T^GATCA
FseI 5111 GGCCGGCC GGCCGG^CC
FspI 1333 TGCGCA TGC^GCA
MfeI 2823 CAATTG C^AATTG
MunI 2823 CAATTG C^AATTG
NheI 2728 GCTAGC G^CTAGC
PinAI 4961 ACCGGT A^CCGGT
PmaCI 1103 CACGTG CAC^GTG
PmlI 1103 CACGTG CAC^GTG
PshBI 3719 ATTAAT AT^TAAT
SapI 4847 GCTCTTC GCTCTTC(1/4)
SexAI 1132 ACCTGGT A^CC[AT]GGT
SmlI 5769 CTTAAG C^T[CT][GA]AG
SspI 5940 AATATT AAT^ATT
XbaI 3318 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1748 GACGCC G[GA]^CG[CT]C
2219 GACGTC
BbvCI 2771 CCTCAGC CCTCAGC(-5/-2)
3758 GCTGAGG
BglI 1534 GCCTGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2918 GCCAGCAGGGC
BsaAI 471 TACGTG [CT]AC^GT[GA]
1103 CACGTG
BsaHI 1748 GACGCC G[GA]^CG[CT]C
2219 GACGTC
BsiEI 1765 CGGCCG CG[GA][CT]^CG
5247 CGGCCG
BspHI 5265 TCATGA T^CATGA
5736 TCATGA
BsrBI 872 CCGCTC CCGCTC(-3/-3)
944 CCGCTC
BsrDI 374 CATTGC GCAATG(2/0)
2607 GCAATG
BssSI 4777 CACGAG CACGAG(-5/-1)
5215 CTCGTG
BstEII 1616 GGTCACC G^GT[ACGT]ACC
1911 GGTTACC
BstPI 1616 GGTCACC G^GT[ACGT]ACC
1911 GGTTACC
DraIII 3625 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
5671 CACCCTGTG
EagI 1765 CGGCCG C^GGCCG
5247 CGGCCG
EclXI 1765 CGGCCG C^GGCCG
5247 CGGCCG
Eco52I 1765 CGGCCG C^GGCCG
5247 CGGCCG
EcoO65I 1616 GGTCACC G^GT[ACGT]ACC
1911 GGTTACC
Hin1I 1748 GACGCC G[GA]^CG[CT]C
2219 GACGTC
NaeI 5112 GCCGGC GCC^GGC
5249 GCCGGC
NcoI 703 CCATGG C^CATGG
5117 CCATGG
NgoMIV 5112 GCCGGC G^CCGGC
5249 GCCGGC
RcaI 5265 TCATGA T^CATGA
5736 TCATGA
SfiI 1533 GGCCTGAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2917 GGCCAGCAGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
Asp700I AspHI AspI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI