##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaLI AseI AsnI Asp700I AviII
BbsI BcgI BciVI BclI BfrI
BpuAI BsiWI BsmI BspDI BsrDI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DraIII EcoRV FbaI FspI
HincII HindII HindIII HpaI MluI
NheI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2605 GGTACC G^GTACC
AccI 4544 GTCTAC GT^[AC][GT]AC
AhdI 125 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 419 GGCGCGCC GG^CGCGCC
Asp718I 2605 GGTACC G^GTACC
AspEI 125 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3351 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5196 GGATCC G^GATCC
BbeI 313 GGCGCC GGCGC^C
BbrPI 1935 CACGTG CAC^GTG
BsaBI 3483 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 5692 TGTACA T^GTACA
BspHI 1706 TCATGA T^CATGA
BspLU11I 1838 ACATGT A^CATGT
BsrGI 5692 TGTACA T^GTACA
BssSI 643 CTCGTG CACGAG(-5/-1)
BstEII 82 GGTGACC G^GT[ACGT]ACC
BstPI 82 GGTGACC G^GT[ACGT]ACC
DrdI 1719 GACAAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 125 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 82 GGTGACC G^GT[ACGT]ACC
EcoT22I 1893 ATGCAT ATGCA^T
FseI 3166 GGCCGGCC GGCCGG^CC
KasI 313 GGCGCC G^GCGCC
KpnI 2605 GGTACC GGTAC^C
MamI 3483 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4270 CAATTG C^AATTG
MunI 4270 CAATTG C^AATTG
NarI 313 GGCGCC GG^CGCC
NdeI 1861 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 1893 ATGCAT ATGCA^T
PaeR7I 3527 CTCGAG C^TCGAG
PflFI 3351 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1935 CACGTG CAC^GTG
PmlI 1935 CACGTG CAC^GTG
RcaI 1706 TCATGA T^CATGA
SfiI 5019 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 313 GGCGCC GGC^GCC
SphI 1487 GCATGC GCATG^C
SspBI 5692 TGTACA T^GTACA
Tth111I 3351 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3527 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 3347 TCCGGA T^CCGGA
5471 TCCGGA
AocI 2245 CCTCAGG CC^T[ACGT]AGG
2600 CCTGAGG
BglII 4409 AGATCT A^GATCT
5066 AGATCT
BsaAI 181 TACGTG [CT]AC^GT[GA]
1935 CACGTG
BseAI 3347 TCCGGA T^CCGGA
5471 TCCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
4664 CGGCCG
BspEI 3347 TCCGGA T^CCGGA
5471 TCCGGA
BssHII 362 GCGCGC G^CGCGC
420 GCGCGC
Bsu36I 2245 CCTCAGG CC^T[ACGT]AGG
2600 CCTGAGG
EagI 2 CGGCCG C^GGCCG
4664 CGGCCG
EclXI 2 CGGCCG C^GGCCG
4664 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
4664 CGGCCG
Eco81I 2245 CCTCAGG CC^T[ACGT]AGG
2600 CCTGAGG
EcoNI 2402 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4176 CCTGCGAGAGG
EcoRI 1844 GAATTC G^AATTC
2815 GAATTC
MroI 3347 TCCGGA T^CCGGA
5471 TCCGGA
MseI 1477 TTAA T^TAA
5713 TTAA
NaeI 917 GCCGGC GCC^GGC
3167 GCCGGC
NcoI 3062 CCATGG C^CATGG
3810 CCATGG
NgoMIV 917 GCCGGC G^CCGGC
3167 GCCGGC
Tru9I 1477 TTAA T^TAA
5713 TTAA
XcmI 3204 CCACGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3870 CCACTCAGACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApaI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI