##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI Aor51HI AscI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BsrFI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
Eco47III EcoNI EcoRV FseI FspI
HaeII Hin1I KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 3945 AACGTT AA^CGTT
AhdI 3259 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4219 GGGCCC GGGCC^C
AseI 5170 ATTAAT AT^TAAT
AsnI 5170 ATTAAT AT^TAAT
AspEI 3259 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 3490 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3248 GCTGAGC GC^T[ACGT]AGC
BpmI 2443 CTCCAG CTGGAG(16/14)
Bpu1102I 3248 GCTGAGC GC^T[ACGT]AGC
BsiEI 2 CGGCCG CG[GA][CT]^CG
BssHII 144 GCGCGC G^CGCGC
BssSI 214 CTCGTG CACGAG(-5/-1)
BstAPI 210 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2508 GGTCACC G^GT[ACGT]ACC
BstPI 2508 GGTCACC G^GT[ACGT]ACC
CelII 3248 GCTGAGC GC^T[ACGT]AGC
DraII 804 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 566 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2 CGGCCG C^GGCCG
Eam1105I 3259 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoO109I 804 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2508 GGTCACC G^GT[ACGT]ACC
HpaI 2776 GTTAAC GTT^AAC
KspI 240 CCGCGG CCGC^GG
NcoI 2436 CCATGG C^CATGG
NheI 733 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PpuMI 804 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 5170 ATTAAT AT^TAAT
Psp1406I 3945 AACGTT AA^CGTT
PstI 205 CTGCAG CTGCA^G
PvuII 1308 CAGCTG CAG^CTG
SacII 240 CCGCGG CCGC^GG
SapI 4302 GCTCTTC GCTCTTC(1/4)
ScaI 4928 AGTACT AGT^ACT
SexAI 567 ACCAGGT A^CC[AT]GGT
SmaI 91 CCCGGG CCC^GGG
SpeI 4684 ACTAGT A^CTAGT
SphI 522 GCATGC GCATG^C
XcmI 2529 CCACTATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 3309 CCTGAGG CC^T[ACGT]AGG
4585 CCTTAGG
BalI 2516 TGGCCA TGG^CCA
3393 TGGCCA
BbsI 2545 GAAGAC GAAGAC(2/6)
1129 GTCTTC
BbvCI 679 GCTGAGG CCTCAGC(-5/-2)
3229 GCTGAGG
BpuAI 2545 GAAGAC GAAGAC(2/6)
1129 GTCTTC
BsaAI 2712 TACGTG [CT]AC^GT[GA]
3749 TACGTG
BsaI 3739 GAGACC GGTCTC(1/5)
2375 GGTCTC
BsmFI 124 GGGAC GGGAC(10/14)
2045 GGGAC
Bsu36I 3309 CCTGAGG CC^T[ACGT]AGG
4585 CCTTAGG
DrdI 126 GACCGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2416 GACTACTTTGTC
EarI 4303 CTCTTC CTCTTC(1/4)
81 GAAGAG
Eco81I 3309 CCTGAGG CC^T[ACGT]AGG
4585 CCTTAGG
EcoRI 1016 GAATTC G^AATTC
4514 GAATTC
Ksp632I 4303 CTCTTC CTCTTC(1/4)
81 GAAGAG
MluNI 2516 TGGCCA TGG^CCA
3393 TGGCCA
MscI 2516 TGGCCA TGG^CCA
3393 TGGCCA
PflMI 1491 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCATGACTTGG
SacI 2132 GAGCTC GAGCT^C
2657 GAGCTC
StuI 2447 AGGCCT AGG^CCT
3233 AGGCCT
SwaI 4720 ATTTAAAT ATTT^AAAT
4838 ATTTAAAT
Van91I 1491 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCATGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BciVI
BclI BcnI BfaI BfrI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI