##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccII AclI AgeI Aor51HI
AscI AseI AsnI AviII BciVI
BclI BsaBI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoO65I EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KspI MamI MfeI MluI
MunI MvnI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SalI ScaI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3789 GGTACC G^GTACC
AccI 1952 GTCTAC GT^[AC][GT]AC
AccIII 165 TCCGGA T^CCGGA
AcyI 219 GGCGCC G[GA]^CG[CT]C
AflII 3215 CTTAAG C^TTAAG
Alw44I 347 GTGCAC G^TGCAC
ApaI 2460 GGGCCC GGGCC^C
ApaLI 347 GTGCAC G^TGCAC
Asp700I 4459 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3789 GGTACC G^GTACC
AspI 1694 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3831 CCTAGG C^CTAGG
BbeI 219 GGCGCC GGCGC^C
BbrPI 1840 CACGTG CAC^GTG
BcgI 2554 CGAGGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3215 CTTAAG C^TTAAG
BglII 2586 AGATCT A^GATCT
BlnI 3831 CCTAGG C^CTAGG
BsaAI 1840 CACGTG [CT]AC^GT[GA]
BsaHI 219 GGCGCC G[GA]^CG[CT]C
BseAI 165 TCCGGA T^CCGGA
BsmI 1770 GCATTC GAATGC(1/-1)
Bsp1407I 4493 TGTACA T^GTACA
BspEI 165 TCCGGA T^CCGGA
BspHI 76 TCATGA T^CATGA
BspLU11I 47 ACATGT A^CATGT
BsrBI 1418 GAGCGG CCGCTC(-3/-3)
BsrGI 4493 TGTACA T^GTACA
BssSI 3442 CTCGTG CACGAG(-5/-1)
EagI 588 CGGCCG C^GGCCG
EclXI 588 CGGCCG C^GGCCG
Eco52I 588 CGGCCG C^GGCCG
FseI 1640 GGCCGGCC GGCCGG^CC
HgaI 2056 GACGC GACGC(5/10)
Hin1I 219 GGCGCC G[GA]^CG[CT]C
KasI 219 GGCGCC G^GCGCC
KpnI 3789 GGTACC GGTAC^C
MroI 165 TCCGGA T^CCGGA
NarI 219 GGCGCC GG^CGCC
PflFI 1694 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1840 CACGTG CAC^GTG
PmlI 1840 CACGTG CAC^GTG
PvuI 1805 CGATCG CGAT^CG
RcaI 76 TCATGA T^CATGA
SapI 485 GAAGAGC GCTCTTC(1/4)
SbfI 2341 CCTGCAGG CCTGCA^GG
SexAI 3557 ACCTGGT A^CC[AT]GGT
SfiI 3635 GGCCCAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 219 GGCGCC GGC^GCC
SphI 1974 GCATGC GCATG^C
Sse8387I 2341 CCTGCAGG CCTGCA^GG
SspBI 4493 TGTACA T^GTACA
Tth111I 1694 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4459 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 47 ACATGT A^C[GA][CT]GT
1841 ACGTGT
AhdI 3916 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4299 GACACGGAGTC
AspEI 3916 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4299 GACACGGAGTC
BamHI 168 GGATCC G^GATCC
226 GGATCC
BbsI 4296 GAAGAC GAAGAC(2/6)
4719 GTCTTC
BpuAI 4296 GAAGAC GAAGAC(2/6)
4719 GTCTTC
BsaWI 165 TCCGGA [AT]^CCGG[AT]
2145 ACCGGA
BsiEI 588 CGGCCG CG[GA][CT]^CG
1805 CGATCG
BsrDI 250 CATTGC GCAATG(2/0)
3492 CATTGC
DsaI 1107 CCATGG C^C[GA][CT]GG
3085 CCATGG
Eam1105I 3916 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4299 GACACGGAGTC
EcoRI 778 GAATTC G^AATTC
4169 GAATTC
NcoI 1107 CCATGG C^CATGG
3085 CCATGG
NheI 2608 GCTAGC G^CTAGC
4029 GCTAGC
PaeR7I 1191 CTCGAG C^TCGAG
1218 CTCGAG
XhoI 1191 CTCGAG C^TCGAG
1218 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII