##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AocI AscI Asp718I AviII AvrII
BbeI BcgI BlnI BsaBI BseAI
BsmBI BspDI BspEI BspHI BspLU11I
BstBI Bsu36I ClaI CpoI DraIII
Eco81I EcoRV FseI FspI KasI
KpnI KspI MamI MroI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I RcaI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 3043 CTTAAG C^TTAAG
Aor51HI 5433 AGCGCT AGC^GCT
Asp700I 4950 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 6019 GGATCC G^GATCC
BbrPI 5167 CACGTG CAC^GTG
BciVI 4774 GTATCC GTATCC(6/5)
BfrI 3043 CTTAAG C^TTAAG
BsaWI 2742 TCCGGT [AT]^CCGG[AT]
BsiWI 1321 CGTACG C^GTACG
BsmI 1350 GAATGC GAATGC(1/-1)
Bsp1407I 1370 TGTACA T^GTACA
BsrGI 1370 TGTACA T^GTACA
BssHII 3005 GCGCGC G^CGCGC
Bst1107I 4852 GTATAC GTA^TAC
BstEII 3321 GGTGACC G^GT[ACGT]ACC
BstPI 3321 GGTGACC G^GT[ACGT]ACC
BstXI 1523 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4852 GTATAC GTA^TAC
Eco47III 5433 AGCGCT AGC^GCT
EcoO65I 3321 GGTGACC G^GT[ACGT]ACC
EcoRI 5287 GAATTC G^AATTC
EcoT22I 3624 ATGCAT ATGCA^T
MfeI 473 CAATTG C^AATTG
MunI 473 CAATTG C^AATTG
NheI 444 GCTAGC G^CTAGC
NsiI 3624 ATGCAT ATGCA^T
PmaCI 5167 CACGTG CAC^GTG
PmlI 5167 CACGTG CAC^GTG
PshAI 714 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2825 CGATCG CGAT^CG
SapI 1238 GCTCTTC GCTCTTC(1/4)
SbfI 600 CCTGCAGG CCTGCA^GG
ScaI 3767 AGTACT AGT^ACT
SexAI 2069 ACCTGGT A^CC[AT]GGT
SmaI 2657 CCCGGG CCC^GGG
SnaBI 5693 TACGTA TAC^GTA
SphI 1862 GCATGC GCATG^C
Sse8387I 600 CCTGCAGG CCTGCA^GG
SspBI 1370 TGTACA T^GTACA
XbaI 5650 TCTAGA T^CTAGA
XcmI 2633 CCAACCTCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2657 CCCGGG C^CCGGG
XmaI 2657 CCCGGG C^CCGGG
XmnI 4950 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 691 GTCTAC GT^[AC][GT]AC
4852 GTATAC
AflIII 1472 ACGCGT A^C[GA][CT]GT
5774 ACGCGT
AhdI 2068 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3642 GACAAATGGTC
AseI 5085 ATTAAT AT^TAAT
5441 ATTAAT
AsnI 5085 ATTAAT AT^TAAT
5441 ATTAAT
AspEI 2068 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3642 GACAAATGGTC
AvaI 1087 CCCGAG C^[CT]CG[GA]G
2657 CCCGGG
BalI 2630 TGGCCA TGG^CCA
3164 TGGCCA
BbsI 594 GAAGAC GAAGAC(2/6)
3945 GTCTTC
BclI 1581 TGATCA T^GATCA
4122 TGATCA
BglII 102 AGATCT A^GATCT
1331 AGATCT
BlpI 823 GCTAAGC GC^T[ACGT]AGC
1066 GCTGAGC
Bpu1102I 823 GCTAAGC GC^T[ACGT]AGC
1066 GCTGAGC
BpuAI 594 GAAGAC GAAGAC(2/6)
3945 GTCTTC
BsaI 2769 GGTCTC GGTCTC(1/5)
5560 GGTCTC
BsoBI 1087 CCCGAG C^[CT]CG[GA]G
2657 CCCGGG
BsrFI 1602 GCCGGC [GA]^CCGG[CT]
3036 GCCGGC
CelII 823 GCTAAGC GC^T[ACGT]AGC
1066 GCTGAGC
Cfr10I 1602 GCCGGC [GA]^CCGG[CT]
3036 GCCGGC
DrdI 3324 GACCACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5413 GACATAACTGTC
Eam1105I 2068 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3642 GACAAATGGTC
EcoNI 4166 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5736 CCTTTTAAAGG
FbaI 1581 TGATCA T^GATCA
4122 TGATCA
HindIII 4566 AAGCTT A^AGCTT
5063 AAGCTT
HpaI 1213 GTTAAC GTT^AAC
6097 GTTAAC
MluI 1472 ACGCGT A^CGCGT
5774 ACGCGT
MluNI 2630 TGGCCA TGG^CCA
3164 TGGCCA
MscI 2630 TGGCCA TGG^CCA
3164 TGGCCA
NaeI 1602 GCCGGC GCC^GGC
3036 GCCGGC
NgoMIV 1602 GCCGGC G^CCGGC
3036 GCCGGC
PpuMI 1913 GGGTCCC [GA]G^G[AT]CC[CT]
3425 GGGTCCC
PshBI 5085 ATTAAT AT^TAAT
5441 ATTAAT
SspI 1003 AATATT AAT^ATT
5515 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AspI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII