##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AhdI Aor51HI AscI
Asp718I AspEI AviII BbeI BbrPI
BcgI BglII BsaAI BsaBI BsiEI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FspI KasI
KpnI KspI MamI MluI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4213 GACGTC GACGT^C
AccI 3823 GTCTAC GT^[AC][GT]AC
AccII 3616 CGCG CG^CG
AccIII 2537 TCCGGA T^CCGGA
AflII 418 CTTAAG C^TTAAG
AflIII 2823 ACATGT A^C[GA][CT]GT
AgeI 472 ACCGGT A^CCGGT
Alw44I 1379 GTGCAC G^TGCAC
ApaLI 1379 GTGCAC G^TGCAC
Asp700I 5319 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1372 CCCGAG C^[CT]CG[GA]G
AvrII 2342 CCTAGG C^CTAGG
BamHI 2191 GGATCC G^GATCC
BanI 2856 GGCACC G^G[CT][GA]CC
BfrI 418 CTTAAG C^TTAAG
BlnI 2342 CCTAGG C^CTAGG
BseAI 2537 TCCGGA T^CCGGA
BsgI 4161 CTGCAC GTGCAG(16/14)
BsoBI 1372 CCCGAG C^[CT]CG[GA]G
BspEI 2537 TCCGGA T^CCGGA
BspHI 5113 TCATGA T^CATGA
BspLU11I 2823 ACATGT A^CATGT
BstEII 3318 GGTCACC G^GT[ACGT]ACC
BstPI 3318 GGTCACC G^GT[ACGT]ACC
BstUI 3616 CGCG CG^CG
DraIII 3422 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 307 GAAGAG CTCTTC(1/4)
EcoO65I 3318 GGTCACC G^GT[ACGT]ACC
EcoRV 1889 GATATC GAT^ATC
FseI 2833 GGCCGGCC GGCCGG^CC
Ksp632I 307 GAAGAG CTCTTC(1/4)
MroI 2537 TCCGGA T^CCGGA
MvnI 3616 CGCG CG^CG
NaeI 2834 GCCGGC GCC^GGC
NgoMIV 2834 GCCGGC G^CCGGC
NheI 410 GCTAGC G^CTAGC
PinAI 472 ACCGGT A^CCGGT
RcaI 5113 TCATGA T^CATGA
SexAI 2698 ACCAGGT A^CC[AT]GGT
SpeI 5125 ACTAGT A^CTAGT
XmnI 5319 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 4213 GACGTC G[GA]^CG[CT]C
4522 GACGCC
ApaI 2407 GGGCCC GGGCC^C
4630 GGGCCC
AseI 851 ATTAAT AT^TAAT
5025 ATTAAT
AsnI 851 ATTAAT AT^TAAT
5025 ATTAAT
AspI 1956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACTCTGTC
BlpI 2519 GCTCAGC GC^T[ACGT]AGC
4435 GCTAAGC
Bpu1102I 2519 GCTCAGC GC^T[ACGT]AGC
4435 GCTAAGC
BsaHI 4213 GACGTC G[GA]^CG[CT]C
4522 GACGCC
BsaI 4084 GAGACC GGTCTC(1/5)
4354 GAGACC
BsmBI 2487 CGTCTC CGTCTC(1/5)
2166 GAGACG
BsmI 3843 GAATGC GAATGC(1/-1)
2453 GCATTC
BspMI 1695 ACCTGC ACCTGC(4/8)
4246 ACCTGC
BsrDI 3373 CATTGC GCAATG(2/0)
2046 GCAATG
BsrFI 472 ACCGGT [GA]^CCGG[CT]
2834 GCCGGC
BstAPI 1690 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3598 GCAGCAGATGC
CelII 2519 GCTCAGC GC^T[ACGT]AGC
4435 GCTAAGC
Cfr10I 472 ACCGGT [GA]^CCGG[CT]
2834 GCCGGC
DraI 4919 TTTAAA TTT^AAA
5100 TTTAAA
EcoNI 2525 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4010 CCTACCTTAGG
EcoRI 910 GAATTC G^AATTC
1899 GAATTC
HaeII 1297 AGCGCC [GA]GCGC^[CT]
1398 AGCGCC
HgaI 3614 GACGC GACGC(5/10)
4522 GACGC
Hin1I 4213 GACGTC G[GA]^CG[CT]C
4522 GACGCC
HindIII 3748 AAGCTT A^AGCTT
5214 AAGCTT
PflFI 1956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACTCTGTC
PpuMI 1976 GGGACCT [GA]G^G[AT]CC[CT]
4399 GGGTCCC
PshAI 2312 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3651 GACATTTGTC
PshBI 851 ATTAAT AT^TAAT
5025 ATTAAT
PvuII 2426 CAGCTG CAG^CTG
2442 CAGCTG
SmlI 418 CTTAAG C^T[CT][GA]AG
4179 CTCAAG
SphI 2620 GCATGC GCATG^C
4113 GCATGC
SspI 2144 AATATT AAT^ATT
4943 AATATT
Tth111I 1956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2098 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BciVI BclI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII