##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcsI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BbrPI BciVI BclI BlnI
BsaAI BsaBI BsiWI BspDI BssHII
BstBI BstEII BstPI ClaI CpoI
DraI DraIII EcoO65I EcoRI EcoRV
EcoT22I FbaI FspI HpaI MamI
MfeI MluI MslI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SfuI SnaBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 5757 GTATAC GT^[AC][GT]AC
AccIII 1452 TCCGGA T^CCGGA
AflII 5885 CTTAAG C^TTAAG
AgeI 2973 ACCGGT A^CCGGT
AocI 4256 CCTGAGG CC^T[ACGT]AGG
BamHI 5173 GGATCC G^GATCC
BfrI 5885 CTTAAG C^TTAAG
BglII 3930 AGATCT A^GATCT
BseAI 1452 TCCGGA T^CCGGA
BspEI 1452 TCCGGA T^CCGGA
BspHI 4704 TCATGA T^CATGA
BspLU11I 2157 ACATGT A^CATGT
BssSI 1871 CTCGTG CACGAG(-5/-1)
Bst1107I 5757 GTATAC GTA^TAC
BstZ17I 5757 GTATAC GTA^TAC
Bsu36I 4256 CCTGAGG CC^T[ACGT]AGG
DrdI 1442 GACGCAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4256 CCTGAGG CC^T[ACGT]AGG
FseI 218 GGCCGGCC GGCCGG^CC
HincII 2093 GTCAAC GT[CT]^[GA]AC
HindII 2093 GTCAAC GT[CT]^[GA]AC
MroI 1452 TCCGGA T^CCGGA
NdeI 5693 CATATG CA^TATG
PflMI 4643 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2973 ACCGGT A^CCGGT
RcaI 4704 TCATGA T^CATGA
SpeI 5808 ACTAGT A^CTAGT
Tsp509I 554 AATT ^AATT
Van91I 4643 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1085 GGTACC G^GTACC
2594 GGTACC
AflIII 2157 ACATGT A^C[GA][CT]GT
5714 ACGTGT
Alw44I 3476 GTGCAC G^TGCAC
5338 GTGCAC
Aor51HI 3891 AGCGCT AGC^GCT
5385 AGCGCT
ApaLI 3476 GTGCAC G^TGCAC
5338 GTGCAC
Asp718I 1085 GGTACC G^GTACC
2594 GGTACC
BcgI 5330 CGACCGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
472 GCAGTCTTTTCG
BsaWI 1452 TCCGGA [AT]^CCGG[AT]
2973 ACCGGT
BsmI 1102 GCATTC GAATGC(1/-1)
5908 GCATTC
Bsp1407I 5399 TGTACA T^GTACA
5689 TGTACA
BsrGI 5399 TGTACA T^GTACA
5689 TGTACA
Eco47III 3891 AGCGCT AGC^GCT
5385 AGCGCT
HindIII 2340 AAGCTT A^AGCTT
3457 AAGCTT
KpnI 1085 GGTACC GGTAC^C
2594 GGTACC
MaeII 984 ACGT A^CGT
5714 ACGT
PvuII 1820 CAGCTG CAG^CTG
2483 CAGCTG
SacI 3095 GAGCTC GAGCT^C
3348 GAGCTC
SbfI 739 CCTGCAGG CCTGCA^GG
872 CCTGCAGG
ScaI 1467 AGTACT AGT^ACT
3078 AGTACT
SexAI 4090 ACCTGGT A^CC[AT]GGT
4182 ACCTGGT
SfiI 218 GGCCGGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
250 GGCCACCCAGGCC
SgrAI 165 CGCCGGCG C[GA]^CCGG[CT]G
419 CGCCGGCG
SphI 1993 GCATGC GCATG^C
3126 GCATGC
Sse8387I 739 CCTGCAGG CCTGCA^GG
872 CCTGCAGG
SspBI 5399 TGTACA T^GTACA
5689 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI