##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI BalI BclI
BfrI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI Eam1105I EcoO65I FbaI FseI
HpaI MluI MluNI MroI MscI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RsrII SbfI SexAI
SfuI SmaI SnaBI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 226 GGTACC G^GTACC
Alw44I 1684 GTGCAC G^TGCAC
Aor51HI 4936 AGCGCT AGC^GCT
ApaLI 1684 GTGCAC G^TGCAC
AseI 5002 ATTAAT AT^TAAT
AsnI 5002 ATTAAT AT^TAAT
Asp718I 226 GGTACC G^GTACC
AspI 5268 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4279 CCTAGG C^CTAGG
BbvCI 5247 GCTGAGG CCTCAGC(-5/-2)
BcgI 1556 CGACGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 73 GTATCC GTATCC(6/5)
BglII 811 AGATCT A^GATCT
BlnI 4279 CCTAGG C^CTAGG
BsaWI 2137 ACCGGA [AT]^CCGG[AT]
BsmBI 1041 CGTCTC CGTCTC(1/5)
BspHI 748 TCATGA T^CATGA
BsrDI 14 CATTGC GCAATG(2/0)
BsrFI 1633 GCCGGC [GA]^CCGG[CT]
Cfr10I 1633 GCCGGC [GA]^CCGG[CT]
DraIII 1346 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1147 CGGCCG C^GGCCG
EclXI 1147 CGGCCG C^GGCCG
Eco47III 4936 AGCGCT AGC^GCT
Eco52I 1147 CGGCCG C^GGCCG
EcoNI 3871 CCTGGGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3538 GAATTC G^AATTC
EcoT22I 5378 ATGCAT ATGCA^T
HincII 920 GTCGAC GT[CT]^[GA]AC
HindII 920 GTCGAC GT[CT]^[GA]AC
HindIII 3108 AAGCTT A^AGCTT
KpnI 226 GGTACC GGTAC^C
MfeI 4458 CAATTG C^AATTG
MunI 4458 CAATTG C^AATTG
NaeI 1633 GCCGGC GCC^GGC
NcoI 3979 CCATGG C^CATGG
NgoMIV 1633 GCCGGC G^CCGGC
NsiI 5378 ATGCAT ATGCA^T
PflFI 5268 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5002 ATTAAT AT^TAAT
RcaI 748 TCATGA T^CATGA
SalI 920 GTCGAC G^TCGAC
SapI 3821 GCTCTTC GCTCTTC(1/4)
SfiI 1174 GGCCAGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1632 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3079 ACTAGT A^CTAGT
SphI 4078 GCATGC GCATG^C
SspI 4639 AATATT AAT^ATT
Tth111I 5268 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1696 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 2240 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
3697 CAGAAGCTG
Asp700I 521 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2162 GAAAGCCTTC
AviII 325 TGCGCA TGC^GCA
433 TGCGCA
BamHI 91 GGATCC G^GATCC
3088 GGATCC
BbrPI 1689 CACGTG CAC^GTG
5019 CACGTG
BbsI 1079 GAAGAC GAAGAC(2/6)
3805 GTCTTC
BlpI 2824 GCTCAGC GC^T[ACGT]AGC
4932 GCTGAGC
Bpu1102I 2824 GCTCAGC GC^T[ACGT]AGC
4932 GCTGAGC
BpuAI 1079 GAAGAC GAAGAC(2/6)
3805 GTCTTC
BsaBI 92 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3356 GATGGAGATC
CelII 2824 GCTCAGC GC^T[ACGT]AGC
4932 GCTGAGC
DraI 4555 TTTAAA TTT^AAA
4834 TTTAAA
EcoRV 3183 GATATC GAT^ATC
4706 GATATC
FspI 325 TGCGCA TGC^GCA
433 TGCGCA
MamI 92 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3356 GATGGAGATC
PmaCI 1689 CACGTG CAC^GTG
5019 CACGTG
PmlI 1689 CACGTG CAC^GTG
5019 CACGTG
PpuMI 1902 GGGACCC [GA]G^G[AT]CC[CT]
5106 GGGACCC
ScaI 3770 AGTACT AGT^ACT
4242 AGTACT
XmnI 521 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2162 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrBI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII