##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I AocI ApaLI AscI
Asp700I Asp718I AspI AvaI AviII
AvrII BbeI BbrPI BbsI BcgI
BciVI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BsrBI
BsrFI BssHII BstBI BstUI Bsu36I
CelII Cfr10I CpoI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoRV
FauI FseI FspI Hin1I KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmlI
PpuMI PshAI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 4108 GTATAC GT^[AC][GT]AC
AclI 1926 AACGTT AA^CGTT
AhdI 1974 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4088 AGCGCT AGC^GCT
ApaI 32 GGGCCC GGGCC^C
AseI 3454 ATTAAT AT^TAAT
AsnI 3454 ATTAAT AT^TAAT
AspEI 1974 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3057 TGGCCA TGG^CCA
BamHI 1533 GGATCC G^GATCC
BanI 3040 GGTGCC G^G[CT][GA]CC
BbvCI 3475 CCTCAGC CCTCAGC(-5/-2)
BclI 1144 TGATCA T^GATCA
BcnI 3044 CCGGG CC^[GC]GG
BglI 785 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1839 CTCCTC GAGGAG(10/8)
BsgI 489 CTGCAC GTGCAG(16/14)
BspDI 2233 ATCGAT AT^CGAT
BspHI 2802 TCATGA T^CATGA
BspMI 2330 ACCTGC ACCTGC(4/8)
Bst1107I 4108 GTATAC GTA^TAC
BstAPI 3723 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1950 GGTTACC G^GT[ACGT]ACC
BstPI 1950 GGTTACC G^GT[ACGT]ACC
BstXI 343 CCATTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4108 GTATAC GTA^TAC
ClaI 2233 ATCGAT AT^CGAT
EaeI 3057 TGGCCA [CT]^GGCC[GA]
Eam1105I 1974 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4088 AGCGCT AGC^GCT
EcoO65I 1950 GGTTACC G^GT[ACGT]ACC
EcoRI 3289 GAATTC G^AATTC
FbaI 1144 TGATCA T^GATCA
HapII 3044 CCGG C^CGG
HgaI 3625 GCGTC GACGC(5/10)
HindIII 1722 AAGCTT A^AGCTT
HpaI 2495 GTTAAC GTT^AAC
HpaII 3044 CCGG C^CGG
MluNI 3057 TGGCCA TGG^CCA
MscI 3057 TGGCCA TGG^CCA
MspI 3044 CCGG C^CGG
NciI 3044 CCGGG CC^[GC]GG
PflMI 196 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1895 GTTTAAAC GTTT^AAAC
PshBI 3454 ATTAAT AT^TAAT
Psp1406I 1926 AACGTT AA^CGTT
RcaI 2802 TCATGA T^CATGA
SapI 3803 GAAGAGC GCTCTTC(1/4)
SexAI 623 ACCAGGT A^CC[AT]GGT
SfaNI 3418 GCATC GCATC(5/9)
SpeI 2437 ACTAGT A^CTAGT
SphI 222 GCATGC GCATG^C
StuI 713 AGGCCT AGG^CCT
TaqI 2234 TCGA T^CGA
TthHB8I 2234 TCGA T^CGA
Van91I 196 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 991 CCATTACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2611 CTTAAG C^TTAAG
3502 CTTAAG
AflIII 380 ACATGT A^C[GA][CT]GT
1371 ACATGT
AlwNI 1400 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3997 CAGAATCTG
AvaII 627 GGTCC G^G[AT]CC
3472 GGACC
BfrI 2611 CTTAAG C^TTAAG
3502 CTTAAG
BsaAI 1383 TACGTA [CT]AC^GT[GA]
2044 TACGTA
BsaI 681 GGTCTC GGTCTC(1/5)
2054 GGTCTC
BsmI 4156 GAATGC GAATGC(1/-1)
64 GCATTC
BspLU11I 380 ACATGT A^CATGT
1371 ACATGT
BsrDI 411 CATTGC GCAATG(2/0)
2162 CATTGC
BssSI 759 CACGAG CACGAG(-5/-1)
1041 CACGAG
DraII 32 GGGCCCT [GA]G^G[ACGT]CC[CT]
115 AGGGCCT
DsaI 640 CCATGG C^C[GA][CT]GG
2970 CCATGG
EcoNI 443 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3818 CCTGGGGGAGG
EcoO109I 32 GGGCCCT [GA]G^G[ACGT]CC[CT]
115 AGGGCCT
HaeII 5 GGCGCT [GA]GCGC^[CT]
4088 AGCGCT
HincII 1445 GTCAAC GT[CT]^[GA]AC
2495 GTTAAC
HindII 1445 GTCAAC GT[CT]^[GA]AC
2495 GTTAAC
HphI 3838 GGTGA GGTGA(8/7)
551 TCACC
MfeI 360 CAATTG C^AATTG
1414 CAATTG
MslI 2341 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3236 CACTGTGGTG
MspA1I 795 CAGCTG C[AC]G^C[GT]G
2525 CAGCTG
MunI 360 CAATTG C^AATTG
1414 CAATTG
NcoI 640 CCATGG C^CATGG
2970 CCATGG
PstI 17 CTGCAG CTGCA^G
2522 CTGCAG
PvuII 795 CAGCTG CAG^CTG
2525 CAGCTG
ScaI 3398 AGTACT AGT^ACT
3926 AGTACT
SnaBI 1383 TACGTA TAC^GTA
2044 TACGTA
SspI 2142 AATATT AAT^ATT
3457 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BfaI
BglII BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfcI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XhoII