##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
AviII BamHI BcgI BclI BsaWI
BseAI BsiWI BspEI BsrFI BssHII
BssSI Bst1107I BstAPI BstZ17I Cfr10I
CpoI DrdI EagI EclXI Eco52I
EcoT22I FbaI FseI FspI KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SalI SbfI SexAI SgrAI SmaI
SnaBI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2332 GGTACC G^GTACC
AhdI 1426 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 804 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1409 AGCGCT AGC^GCT
ApaI 1504 GGGCCC GGGCC^C
Asp700I 5369 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2332 GGTACC G^GTACC
AspEI 1426 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2496 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1835 CCCGAG C^[CT]CG[GA]G
AvrII 5233 CCTAGG C^CTAGG
BbeI 1817 GGCGCC GGCGC^C
BbvCI 2419 GCTGAGG CCTCAGC(-5/-2)
BciVI 5009 GGATAC GTATCC(6/5)
BlnI 5233 CCTAGG C^CTAGG
BlpI 5238 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5238 GCTCAGC GC^T[ACGT]AGC
BsaBI 5010 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1390 CTGCAC GTGCAG(16/14)
BsoBI 1835 CCCGAG C^[CT]CG[GA]G
BspHI 474 TCATGA T^CATGA
BstBI 704 TTCGAA TT^CGAA
Bsu36I 804 CCTGAGG CC^T[ACGT]AGG
CelII 5238 GCTCAGC GC^T[ACGT]AGC
DraI 2251 TTTAAA TTT^AAA
Eam1105I 1426 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1409 AGCGCT AGC^GCT
Eco81I 804 CCTGAGG CC^T[ACGT]AGG
EcoNI 4410 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4234 GATATC GAT^ATC
KasI 1817 GGCGCC G^GCGCC
KpnI 2332 GGTACC GGTAC^C
MamI 5010 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1817 GGCGCC GG^CGCC
NcoI 582 CCATGG C^CATGG
NspV 704 TTCGAA TT^CGAA
PflFI 2496 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3754 CGATCG CGAT^CG
RcaI 474 TCATGA T^CATGA
SacI 1344 GAGCTC GAGCT^C
SapI 1100 GCTCTTC GCTCTTC(1/4)
SfiI 2797 GGCCACAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1817 GGCGCC GGC^GCC
SfuI 704 TTCGAA TT^CGAA
SpeI 4382 ACTAGT A^CTAGT
Tth111I 2496 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5377 TCTAGA T^CTAGA
XmnI 5369 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 27 CGCG CG^CG
3149 CGCG
AflII 3563 CTTAAG C^TTAAG
4088 CTTAAG
AlwI 988 GATCC GGATC(4/5)
3702 GATCC
BbrPI 1557 CACGTG CAC^GTG
3216 CACGTG
BfrI 3563 CTTAAG C^TTAAG
4088 CTTAAG
BglII 1127 AGATCT A^GATCT
1686 AGATCT
BsaAI 1557 CACGTG [CT]AC^GT[GA]
3216 CACGTG
BsiEI 1173 CGGTCG CG[GA][CT]^CG
3754 CGATCG
BsmI 1806 GAATGC GAATGC(1/-1)
5536 GCATTC
BspDI 1049 ATCGAT AT^CGAT
3752 ATCGAT
BspLU11I 968 ACATGT A^CATGT
3633 ACATGT
BsrBI 30 GAGCGG CCGCTC(-3/-3)
1954 GAGCGG
BstEII 1515 GGTCACC G^GT[ACGT]ACC
1706 GGTCACC
BstPI 1515 GGTCACC G^GT[ACGT]ACC
1706 GGTCACC
BstUI 27 CGCG CG^CG
3149 CGCG
BstXI 653 CCAGGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2547 CCACAACAATGG
ClaI 1049 ATCGAT AT^CGAT
3752 ATCGAT
DraIII 4521 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4889 CACTCAGTG
DsaI 582 CCATGG C^C[GA][CT]GG
1702 CCACGG
EcoO65I 1515 GGTCACC G^GT[ACGT]ACC
1706 GGTCACC
HindIII 2039 AAGCTT A^AGCTT
3846 AAGCTT
MvnI 27 CGCG CG^CG
3149 CGCG
PflMI 252 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCAGCTCCTGG
PmaCI 1557 CACGTG CAC^GTG
3216 CACGTG
PmlI 1557 CACGTG CAC^GTG
3216 CACGTG
ScaI 1375 AGTACT AGT^ACT
4973 AGTACT
StuI 3410 AGGCCT AGG^CCT
4422 AGGCCT
Van91I 252 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
792 CCAGCTCCTGG
XcmI 1386 CCAACTGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3314 CCATAGGTGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII