##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI AscI AseI AsnI AspEI
AvrII BciVI BfrI BlnI BlpI
Bpu1102I BsaBI BsiWI BspDI Bst1107I
BstZ17I Bsu36I CelII ClaI DraI
Eam1105I Eco81I EcoRV EcoT22I FseI
HindIII HpaI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI SacII
SalI SapI SfiI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Aor51HI 1863 AGCGCT AGC^GCT
Asp700I 1816 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1278 TGCGCA TGC^GCA
BamHI 1557 GGATCC G^GATCC
BbvCI 1096 CCTCAGC CCTCAGC(-5/-2)
BcgI 1022 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1766 CTAG C^TAG
BglII 2028 AGATCT A^GATCT
BsmI 2209 GAATGC GAATGC(1/-1)
BspHI 2229 TCATGA T^CATGA
BstAPI 844 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 512 TTCGAA TT^CGAA
CpoI 1244 CGGACCG CG^G[AT]CCG
DraIII 2219 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1669 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1863 AGCGCT AGC^GCT
EcoRI 515 GAATTC G^AATTC
FspI 1278 TGCGCA TGC^GCA
MaeI 1766 CTAG C^TAG
MseI 1836 TTAA T^TAA
NcoI 3318 CCATGG C^CATGG
NspV 512 TTCGAA TT^CGAA
PaeR7I 2819 CTCGAG C^TCGAG
RcaI 2229 TCATGA T^CATGA
RsrII 1244 CGGACCG CG^G[AT]CCG
SacI 458 GAGCTC GAGCT^C
SbfI 2452 CCTGCAGG CCTGCA^GG
ScaI 780 AGTACT AGT^ACT
SexAI 993 ACCTGGT A^CC[AT]GGT
SfuI 512 TTCGAA TT^CGAA
Sse8387I 2452 CCTGCAGG CCTGCA^GG
StuI 828 AGGCCT AGG^CCT
Tru9I 1836 TTAA T^TAA
XbaI 1765 TCTAGA T^CTAGA
XhoI 2819 CTCGAG C^TCGAG
XmnI 1816 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1394 GGTACC G^GTACC
3626 GGTACC
AccIII 870 TCCGGA T^CCGGA
1554 TCCGGA
AcyI 186 GGCGCC G[GA]^CG[CT]C
2606 GGCGCC
AflIII 858 ACATGT A^C[GA][CT]GT
1533 ACATGT
Asp718I 1394 GGTACC G^GTACC
3626 GGTACC
AspI 959 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3560 GACCGCGTC
BbeI 186 GGCGCC GGCGC^C
2606 GGCGCC
BbrPI 2264 CACGTG CAC^GTG
2912 CACGTG
BclI 2232 TGATCA T^GATCA
2916 TGATCA
BsaHI 186 GGCGCC G[GA]^CG[CT]C
2606 GGCGCC
BseAI 870 TCCGGA T^CCGGA
1554 TCCGGA
BsmBI 964 CGTCTC CGTCTC(1/5)
1653 GAGACG
BspEI 870 TCCGGA T^CCGGA
1554 TCCGGA
BspLU11I 858 ACATGT A^CATGT
1533 ACATGT
EcoNI 2781 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2797 CCTGAGCCAGG
FbaI 2232 TGATCA T^GATCA
2916 TGATCA
Hin1I 186 GGCGCC G[GA]^CG[CT]C
2606 GGCGCC
KasI 186 GGCGCC G^GCGCC
2606 GGCGCC
KpnI 1394 GGTACC GGTAC^C
3626 GGTACC
MroI 870 TCCGGA T^CCGGA
1554 TCCGGA
NarI 186 GGCGCC GG^CGCC
2606 GGCGCC
PflFI 959 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3560 GACCGCGTC
PflMI 2218 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3759 CCATCCGCTGG
PmaCI 2264 CACGTG CAC^GTG
2912 CACGTG
PmlI 2264 CACGTG CAC^GTG
2912 CACGTG
SfoI 186 GGCGCC GGC^GCC
2606 GGCGCC
SphI 2258 GCATGC GCATG^C
2617 GCATGC
TfiI 1130 GATTC G^A[AT]TC
1251 GATTC
Tth111I 959 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3560 GACCGCGTC
Van91I 2218 CCACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3759 CCATCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI