##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BciVI BlnI BsaAI
BsaHI BseAI BseRI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MroI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2289 GTAGAC GT^[AC][GT]AC
AflIII 8 ACGCGT A^C[GA][CT]GT
Alw44I 2096 GTGCAC G^TGCAC
AlwNI 2256 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2888 CCTCAGG CC^T[ACGT]AGG
ApaLI 2096 GTGCAC G^TGCAC
AseI 1459 ATTAAT AT^TAAT
AsnI 1459 ATTAAT AT^TAAT
Asp700I 359 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2748 CCCGGG C^[CT]CG[GA]G
BanI 2578 GGTGCC G^G[CT][GA]CC
BanII 2383 GGGCTC G[GA]GC[CT]^C
BbvI 3033 GCAGC GCAGC(8/12)
BcgI 2895 CGATCCATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 24 TGATCA T^GATCA
BglI 2376 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2957 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2957 GCTCAGC GC^T[ACGT]AGC
BsaWI 2647 ACCGGA [AT]^CCGG[AT]
BsmBI 2675 GAGACG CGTCTC(1/5)
BsoBI 2748 CCCGGG C^[CT]CG[GA]G
Bsu36I 2888 CCTCAGG CC^T[ACGT]AGG
CelII 2957 GCTCAGC GC^T[ACGT]AGC
DraIII 1344 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3 GACCCACGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2574 CCATGG C^C[GA][CT]GG
Eco81I 2888 CCTCAGG CC^T[ACGT]AGG
EcoNI 1967 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2574 CCATGG C^C[AT][AT]GG
EcoT22I 223 ATGCAT ATGCA^T
FbaI 24 TGATCA T^GATCA
Fnu4HI 3033 GCAGC GC^[ACGT]GC
HgaI 10 GCGTC GACGC(5/10)
ItaI 3033 GCAGC GC^[ACGT]GC
MluI 8 ACGCGT A^CGCGT
NcoI 2574 CCATGG C^CATGG
NsiI 223 ATGCAT ATGCA^T
PshBI 1459 ATTAAT AT^TAAT
SapI 267 GCTCTTC GCTCTTC(1/4)
SexAI 1043 ACCTGGT A^CC[AT]GGT
SmaI 2748 CCCGGG CCC^GGG
SspI 1997 AATATT AAT^ATT
StyI 2574 CCATGG C^C[AT][AT]GG
TseI 3033 GCAGC G^C[AT]GC
XmaCI 2748 CCCGGG C^CCGGG
XmaI 2748 CCCGGG C^CCGGG
XmnI 359 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 9 CGCG CG^CG
2718 CGCG
AflII 2150 CTTAAG C^TTAAG
2969 CTTAAG
AlwI 2896 GATCC GGATC(4/5)
2650 GGATC
AspHI 91 GAGCAC G[AT]GC[AT]^C
2096 GTGCAC
AvaII 1619 GGACC G^G[AT]CC
1897 GGTCC
BcnI 2748 CCCGG CC^[GC]GG
2749 CCGGG
BfrI 2150 CTTAAG C^TTAAG
2969 CTTAAG
BglII 770 AGATCT A^GATCT
1274 AGATCT
BsaBI 1680 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1748 GATGTATATC
BsgI 2365 CTGCAC GTGCAG(16/14)
1350 GTGCAG
BsiHKAI 91 GAGCAC G[AT]GC[AT]^C
2096 GTGCAC
BsmFI 1413 GTCCC GGGAC(10/14)
1898 GTCCC
BsmI 221 GAATGC GAATGC(1/-1)
1804 GCATTC
BspMI 2260 ACCTGC ACCTGC(4/8)
2500 GCAGGT
BstAPI 1343 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2255 GCAGTACCTGC
BstBI 356 TTCGAA TT^CGAA
1854 TTCGAA
BstUI 9 CGCG CG^CG
2718 CGCG
BstYI 770 AGATCT [GA]^GATC[CT]
1274 AGATCT
Cfr13I 1619 GGACC G^G[ACGT]CC
1897 GGTCC
EarI 268 CTCTTC CTCTTC(1/4)
2173 GAAGAG
HapII 2648 CCGG C^CGG
2749 CCGG
HpaII 2648 CCGG C^CGG
2749 CCGG
Ksp632I 268 CTCTTC CTCTTC(1/4)
2173 GAAGAG
MaeII 2433 ACGT A^CGT
2858 ACGT
MamI 1680 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1748 GATGTATATC
MflI 770 AGATCT [GA]^GATC[CT]
1274 AGATCT
MspI 2648 CCGG C^CGG
2749 CCGG
MvnI 9 CGCG CG^CG
2718 CGCG
NciI 2748 CCCGG CC^[GC]GG
2749 CCGGG
NlaIV 1897 GGTCCC GG[ACGT]^[ACGT]CC
2578 GGTGCC
NspV 356 TTCGAA TT^CGAA
1854 TTCGAA
Sau96I 1619 GGACC G^G[ACGT]CC
1897 GGTCC
SfuI 356 TTCGAA TT^CGAA
1854 TTCGAA
XhoII 770 AGATCT [GA]^GATC[CT]
1274 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspI BalI BbsI BbvCI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsiYI BslI BsmAI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI DdeI DpnII
EaeI EcoRI EcoRII FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
MwoI NdeII NlaIII PflFI PleI
RcaI RsaI Sau3AI ScrFI SfaNI
SfcI SmlI StuI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
TthHB8I