##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AocI ApoI AscI AseI AsnI
BalI BciVI BfrI BglII BlpI
Bpu1102I BsaBI BsiWI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KspI MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI ScaI SfuI SgrAI
SmlI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3377 GTCGAC GT^[AC][GT]AC
AccIII 3254 TCCGGA T^CCGGA
AhdI 3385 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1350 AGCGCT AGC^GCT
Asp700I 1626 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3385 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 470 TGCGCA TGC^GCA
AvrII 2662 CCTAGG C^CTAGG
BamHI 524 GGATCC G^GATCC
BbvCI 1838 CCTCAGC CCTCAGC(-5/-2)
BlnI 2662 CCTAGG C^CTAGG
BseAI 3254 TCCGGA T^CCGGA
BsiEI 516 CGGCCG CG[GA][CT]^CG
BsmBI 2574 CGTCTC CGTCTC(1/5)
BsmI 2163 GCATTC GAATGC(1/-1)
BspEI 3254 TCCGGA T^CCGGA
BssHII 606 GCGCGC G^CGCGC
BstXI 1208 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 524 GGATCC [GA]^GATC[CT]
EagI 516 CGGCCG C^GGCCG
Eam1105I 3385 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 516 CGGCCG C^GGCCG
Eco47III 1350 AGCGCT AGC^GCT
Eco52I 516 CGGCCG C^GGCCG
EcoNI 1038 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 470 TGCGCA TGC^GCA
MflI 524 GGATCC [GA]^GATC[CT]
MroI 3254 TCCGGA T^CCGGA
MseI 3009 TTAA T^TAA
NaeI 1118 GCCGGC GCC^GGC
NcoI 1274 CCATGG C^CATGG
NgoMIV 1118 GCCGGC G^CCGGC
SalI 3377 GTCGAC G^TCGAC
SexAI 3404 ACCAGGT A^CC[AT]GGT
SfiI 639 GGCCACGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3298 CCCGGG CCC^GGG
SphI 793 GCATGC GCATG^C
StuI 1449 AGGCCT AGG^CCT
Tru9I 3009 TTAA T^TAA
XhoII 524 GGATCC [GA]^GATC[CT]
XmaCI 3298 CCCGGG C^CCGGG
XmaI 3298 CCCGGG C^CCGGG
XmnI 1626 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 1601 GGTACC G^GTACC
2592 GGTACC
AlwI 525 GATCC GGATC(4/5)
524 GGATC
Asp718I 1601 GGTACC G^GTACC
2592 GGTACC
AspI 688 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACACAGTC
BbsI 2430 GAAGAC GAAGAC(2/6)
694 GTCTTC
BcgI 2178 GCAGAACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3127 GCAGACTCGTCG
BpuAI 2430 GAAGAC GAAGAC(2/6)
694 GTCTTC
BsaI 1989 GAGACC GGTCTC(1/5)
3163 GGTCTC
BstAPI 225 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
547 GCACACGATGC
HgaI 814 GACGC GACGC(5/10)
832 GACGC
HincII 3117 GTCAAC GT[CT]^[GA]AC
3377 GTCGAC
HindII 3117 GTCAAC GT[CT]^[GA]AC
3377 GTCGAC
KpnI 1601 GGTACC GGTAC^C
2592 GGTACC
PflFI 688 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACACAGTC
TaqI 2436 TCGA T^CGA
3378 TCGA
Tth111I 688 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACACAGTC
TthHB8I 2436 TCGA T^CGA
3378 TCGA
XcmI 389 CCATCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3070 CCACCCACACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwNI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbrPI BbvI BclI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI Sse8387I StyI TfiI TseI
Tsp45I Tsp509I TspRI Van91I