##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI ApaI AscI Asp718I
AviII AvrII BbeI BbrPI BcgI
BclI BglI BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BspMI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI Sse8387I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1815 GTAGAC GT^[AC][GT]AC
AflIII 1698 ACATGT A^C[GA][CT]GT
AhdI 100 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4477 GTGCAC G^TGCAC
AlwNI 1902 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4477 GTGCAC G^TGCAC
AspEI 100 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 4781 GGACC G^G[AT]CC
BanII 3063 GGGCTC G[GA]GC[CT]^C
BbsI 1188 GAAGAC GAAGAC(2/6)
BbvCI 1937 CCTCAGC CCTCAGC(-5/-2)
BcnI 3219 CCGGG CC^[GC]GG
BglII 1538 AGATCT A^GATCT
BlpI 2157 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2157 GCTTAGC GC^T[ACGT]AGC
BpuAI 1188 GAAGAC GAAGAC(2/6)
BsaWI 1237 ACCGGA [AT]^CCGG[AT]
Bsp1407I 4695 TGTACA T^GTACA
BspLU11I 1698 ACATGT A^CATGT
BsrBI 3308 GAGCGG CCGCTC(-3/-3)
BsrGI 4695 TGTACA T^GTACA
BstEII 1704 GGTTACC G^GT[ACGT]ACC
BstPI 1704 GGTTACC G^GT[ACGT]ACC
CelII 2157 GCTTAGC GC^T[ACGT]AGC
Eam1105I 100 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1704 GGTTACC G^GT[ACGT]ACC
EcoRV 3454 GATATC GAT^ATC
EcoT14I 663 CCATGG C^C[AT][AT]GG
HgaI 1118 GACGC GACGC(5/10)
NciI 3219 CCGGG CC^[GC]GG
NcoI 663 CCATGG C^CATGG
NheI 189 GCTAGC G^CTAGC
NruI 4383 TCGCGA TCG^CGA
PaeR7I 4528 CTCGAG C^TCGAG
PstI 1899 CTGCAG CTGCA^G
SnaBI 534 TACGTA TAC^GTA
SphI 751 GCATGC GCATG^C
SspBI 4695 TGTACA T^GTACA
StyI 663 CCATGG C^C[AT][AT]GG
SwaI 4839 ATTTAAAT ATTT^AAAT
XbaI 4787 TCTAGA T^CTAGA
XhoI 4528 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflII 606 CTTAAG C^TTAAG
2854 CTTAAG
AocI 2674 CCTGAGG CC^T[ACGT]AGG
3146 CCTGAGG
AseI 3973 ATTAAT AT^TAAT
4760 ATTAAT
AsnI 3973 ATTAAT AT^TAAT
4760 ATTAAT
AspI 1844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4207 GACGGGGTC
AvaI 4283 CTCGGG C^[CT]CG[GA]G
4528 CTCGAG
BalI 2119 TGGCCA TGG^CCA
2230 TGGCCA
BamHI 893 GGATCC G^GATCC
2486 GGATCC
BanI 1966 GGTGCC G^G[CT][GA]CC
4512 GGCACC
BciVI 667 GGATAC GTATCC(6/5)
1788 GTATCC
BfrI 606 CTTAAG C^TTAAG
2854 CTTAAG
BsaAI 534 TACGTA [CT]AC^GT[GA]
2654 TACGTG
BseRI 4656 CTCCTC GAGGAG(10/8)
2677 GAGGAG
BsgI 95 GTGCAG GTGCAG(16/14)
1875 GTGCAG
BsoBI 4283 CTCGGG C^[CT]CG[GA]G
4528 CTCGAG
BsrDI 755 GCAATG GCAATG(2/0)
923 GCAATG
Bsu36I 2674 CCTGAGG CC^T[ACGT]AGG
3146 CCTGAGG
CfoI 2114 GCGC GCG^C
3319 GCGC
DsaI 58 CCGTGG C^C[GA][CT]GG
663 CCATGG
EaeI 2119 TGGCCA [CT]^GGCC[GA]
2230 TGGCCA
Eco81I 2674 CCTGAGG CC^T[ACGT]AGG
3146 CCTGAGG
HapII 1238 CCGG C^CGG
3219 CCGG
HhaI 2114 GCGC GCG^C
3319 GCGC
HinP1I 2114 GCGC G^CGC
3319 GCGC
HpaII 1238 CCGG C^CGG
3219 CCGG
MluNI 2119 TGGCCA TGG^CCA
2230 TGGCCA
MscI 2119 TGGCCA TGG^CCA
2230 TGGCCA
MspA1I 296 CAGCTG C[AC]G^C[GT]G
3648 CAGCTG
MspI 1238 CCGG C^CGG
3219 CCGG
NspI 751 GCATGC [GA]CATG^[CT]
1698 ACATGT
PflFI 1844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4207 GACGGGGTC
PflMI 168 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4406 CCAACTCTTGG
PshBI 3973 ATTAAT AT^TAAT
4760 ATTAAT
PvuII 296 CAGCTG CAG^CTG
3648 CAGCTG
SapI 3700 GAAGAGC GCTCTTC(1/4)
2953 GCTCTTC
ScaI 3896 AGTACT AGT^ACT
4669 AGTACT
StuI 938 AGGCCT AGG^CCT
1442 AGGCCT
Tth111I 1844 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4207 GACGGGGTC
Van91I 168 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4406 CCAACTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI BbvI
BfaI BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT22I FauI Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MvnI MwoI NdeI NdeII NlaIII
NlaIV NsiI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI