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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BglI BlnI BlpI
Bpu1102I BsaHI BseAI BseRI BsiEI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SphI Sse8387I SspBI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2447 GTAGAC GT^[AC][GT]AC
AflII 2942 CTTAAG C^TTAAG
Alw44I 4068 GTGCAC G^TGCAC
AlwNI 71 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4068 GTGCAC G^TGCAC
AseI 1103 ATTAAT AT^TAAT
AsnI 1103 ATTAAT AT^TAAT
AspI 1088 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 87 CCCGGG C^[CT]CG[GA]G
BbsI 1085 GAAGAC GAAGAC(2/6)
BciVI 3252 GGATAC GTATCC(6/5)
BfrI 2942 CTTAAG C^TTAAG
BglII 3812 AGATCT A^GATCT
BpmI 321 CTCCAG CTGGAG(16/14)
BpuAI 1085 GAAGAC GAAGAC(2/6)
BsaWI 2515 TCCGGT [AT]^CCGG[AT]
BsgI 1421 GTGCAG GTGCAG(16/14)
BsiWI 2390 CGTACG C^GTACG
BsoBI 87 CCCGGG C^[CT]CG[GA]G
BspMI 4272 GCAGGT ACCTGC(4/8)
BstNI 4202 CCTGG CC^[AT]GG
DraIII 1251 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 38 GACCCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 4202 CCTGG ^CC[AT]GG
EcoRV 3750 GATATC GAT^ATC
FauI 24 GCGGG CCCGC(4/6)
HincII 513 GTCAAC GT[CT]^[GA]AC
HindII 513 GTCAAC GT[CT]^[GA]AC
MfeI 4485 CAATTG C^AATTG
MspA1I 690 CAGCTG C[AC]G^C[GT]G
MunI 4485 CAATTG C^AATTG
MvaI 4202 CCTGG CC^[AT]GG
NdeI 530 CATATG CA^TATG
NheI 92 GCTAGC G^CTAGC
PflFI 1088 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1103 ATTAAT AT^TAAT
PstI 77 CTGCAG CTGCA^G
PvuII 690 CAGCTG CAG^CTG
SacI 1213 GAGCTC GAGCT^C
SmaI 87 CCCGGG CCC^GGG
SpeI 2960 ACTAGT A^CTAGT
StuI 2698 AGGCCT AGG^CCT
Tth111I 1088 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4073 CCACATTATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 256 TGGCCA TGG^CCA
958 TGGCCA
BclI 3385 TGATCA T^GATCA
4450 TGATCA
BsaAI 2388 TACGTA [CT]AC^GT[GA]
2392 TACGTA
BsaBI 743 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2299 GATAAAAATC
BsaI 930 GAGACC GGTCTC(1/5)
2209 GGTCTC
BsmI 3442 GAATGC GAATGC(1/-1)
3501 GAATGC
BstXI 961 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4235 CCAAGTATCTGG
CfoI 135 GCGC GCG^C
245 GCGC
DraII 3900 GGGTCCT [GA]G^G[ACGT]CC[CT]
4342 GGGACCT
DsaI 248 CCATGG C^C[GA][CT]GG
3863 CCATGG
EcoO109I 3900 GGGTCCT [GA]G^G[ACGT]CC[CT]
4342 GGGACCT
EcoT22I 2817 ATGCAT ATGCA^T
4440 ATGCAT
FbaI 3385 TGATCA T^GATCA
4450 TGATCA
HhaI 135 GCGC GCG^C
245 GCGC
HinP1I 135 GCGC G^CGC
245 GCGC
MamI 743 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2299 GATAAAAATC
MluNI 256 TGGCCA TGG^CCA
958 TGGCCA
MscI 256 TGGCCA TGG^CCA
958 TGGCCA
NcoI 248 CCATGG C^CATGG
3863 CCATGG
NsiI 2817 ATGCAT ATGCA^T
4440 ATGCAT
PflMI 248 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2157 CCATTCCTTGG
PleI 1508 GAGTC GAGTC(4/5)
4548 GAGTC
PpuMI 3900 GGGTCCT [GA]G^G[AT]CC[CT]
4342 GGGACCT
ScaI 2946 AGTACT AGT^ACT
4286 AGTACT
SmlI 299 CTTGAG C^T[CT][GA]AG
2942 CTTAAG
SnaBI 2388 TACGTA TAC^GTA
2392 TACGTA
SwaI 1780 ATTTAAAT ATTT^AAAT
4226 ATTTAAAT
Van91I 248 CCATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2157 CCATTCCTTGG
XbaI 2381 TCTAGA T^CTAGA
3978 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BsrDI
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRI EcoT14I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MwoI NciI
NdeII NlaIII NlaIV NspI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI