##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AvrII BbrPI BlnI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII Bst1107I BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI MluI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SbfI
SfiI SnaBI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 6590 GGTACC G^GTACC
AccIII 6060 TCCGGA T^CCGGA
AflIII 732 ACATGT A^C[GA][CT]GT
Alw44I 3400 GTGCAC G^TGCAC
ApaI 244 GGGCCC GGGCC^C
ApaLI 3400 GTGCAC G^TGCAC
Asp718I 6590 GGTACC G^GTACC
AspI 7175 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 129 CCCGGG C^[CT]CG[GA]G
AviII 141 TGCGCA TGC^GCA
BbeI 5649 GGCGCC GGCGC^C
BbvCI 7013 GCTGAGG CCTCAGC(-5/-2)
BcgI 6706 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 5813 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5813 GCTGAGC GC^T[ACGT]AGC
BsaAI 2304 TACGTG [CT]AC^GT[GA]
BsaBI 1898 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 6060 TCCGGA [AT]^CCGG[AT]
BseAI 6060 TCCGGA T^CCGGA
BsoBI 129 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2008 TGTACA T^GTACA
BspEI 6060 TCCGGA T^CCGGA
BspLU11I 732 ACATGT A^CATGT
BsrGI 2008 TGTACA T^GTACA
BstBI 1950 TTCGAA TT^CGAA
BstEII 1124 GGTAACC G^GT[ACGT]ACC
BstPI 1124 GGTAACC G^GT[ACGT]ACC
CelII 5813 GCTGAGC GC^T[ACGT]AGC
EcoO65I 1124 GGTAACC G^GT[ACGT]ACC
EcoRI 633 GAATTC G^AATTC
EcoRV 437 GATATC GAT^ATC
FspI 141 TGCGCA TGC^GCA
HgaI 205 GACGC GACGC(5/10)
HpaI 956 GTTAAC GTT^AAC
KasI 5649 GGCGCC G^GCGCC
KpnI 6590 GGTACC GGTAC^C
KspI 57 CCGCGG CCGC^GG
MamI 1898 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 6060 TCCGGA T^CCGGA
NaeI 38 GCCGGC GCC^GGC
NarI 5649 GGCGCC GG^CGCC
NdeI 693 CATATG CA^TATG
NgoMIV 38 GCCGGC G^CCGGC
NruI 21 TCGCGA TCG^CGA
NspV 1950 TTCGAA TT^CGAA
PflFI 7175 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2442 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 6350 GAGCTC GAGCT^C
SacII 57 CCGCGG CCGC^GG
SexAI 3558 ACCTGGT A^CC[AT]GGT
SfoI 5649 GGCGCC GGC^GCC
SfuI 1950 TTCGAA TT^CGAA
SgrAI 37 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 129 CCCGGG CCC^GGG
SphI 1377 GCATGC GCATG^C
SspBI 2008 TGTACA T^GTACA
Tth111I 7175 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2442 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 129 CCCGGG C^CCGGG
XmaI 129 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 22 CGCG CG^CG
58 CGCG
AcyI 205 GACGCC G[GA]^CG[CT]C
5649 GGCGCC
AflII 4207 CTTAAG C^TTAAG
6131 CTTAAG
Asp700I 908 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7482 GAAACTTTTC
BamHI 3334 GGATCC G^GATCC
6580 GGATCC
BbsI 728 GAAGAC GAAGAC(2/6)
2864 GAAGAC
BciVI 2917 GGATAC GTATCC(6/5)
4951 GGATAC
BclI 1696 TGATCA T^GATCA
1705 TGATCA
BfrI 4207 CTTAAG C^TTAAG
6131 CTTAAG
BglI 237 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5267 GCCCATGTGGC
BpuAI 728 GAAGAC GAAGAC(2/6)
2864 GAAGAC
BsaHI 205 GACGCC G[GA]^CG[CT]C
5649 GGCGCC
BsaI 1367 GAGACC GGTCTC(1/5)
1428 GAGACC
BspHI 1515 TCATGA T^CATGA
1959 TCATGA
BspMI 7642 ACCTGC ACCTGC(4/8)
7325 GCAGGT
BsrFI 38 GCCGGC [GA]^CCGG[CT]
5694 GCCGGT
BssSI 6704 CACGAG CACGAG(-5/-1)
6155 CTCGTG
BstUI 22 CGCG CG^CG
58 CGCG
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
5694 GCCGGT
DraIII 1399 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
6262 CACTCTGTG
FbaI 1696 TGATCA T^GATCA
1705 TGATCA
Hin1I 205 GACGCC G[GA]^CG[CT]C
5649 GGCGCC
MfeI 1826 CAATTG C^AATTG
2264 CAATTG
MunI 1826 CAATTG C^AATTG
2264 CAATTG
MvnI 22 CGCG CG^CG
58 CGCG
NheI 3167 GCTAGC G^CTAGC
7321 GCTAGC
RcaI 1515 TCATGA T^CATGA
1959 TCATGA
SalI 3120 GTCGAC G^TCGAC
5973 GTCGAC
ScaI 559 AGTACT AGT^ACT
4179 AGTACT
SpeI 5483 ACTAGT A^CTAGT
7560 ACTAGT
XmnI 908 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7482 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII