##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccIII AclI AgeI AhdI AlwNI
Aor51HI ApaI AscI AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BcgI BcnI BglI
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspEI
BspMI BsrBI BssHII BssSI BstEII
BstPI BstXI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI HaeII HgaI HpaI KasI
KspI MfeI MluI MroI MunI
NarI NciI NcoI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1923 GACGTC GACGT^C
Acc65I 1859 GGTACC G^GTACC
AccII 1051 CGCG CG^CG
AcyI 1923 GACGTC G[GA]^CG[CT]C
Alw44I 464 GTGCAC G^TGCAC
AocI 2938 CCTAAGG CC^T[ACGT]AGG
ApaLI 464 GTGCAC G^TGCAC
AseI 1816 ATTAAT AT^TAAT
AsnI 1816 ATTAAT AT^TAAT
Asp718I 1859 GGTACC G^GTACC
BanI 1859 GGTACC G^G[CT][GA]CC
BbvCI 3446 GCTGAGG CCTCAGC(-5/-2)
BciVI 1552 GTATCC GTATCC(6/5)
BglII 1101 AGATCT A^GATCT
BlpI 1197 GCTTAGC GC^T[ACGT]AGC
BpmI 341 CTCCAG CTGGAG(16/14)
Bpu1102I 1197 GCTTAGC GC^T[ACGT]AGC
BsaBI 882 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1923 GACGTC G[GA]^CG[CT]C
BseRI 3449 GAGGAG GAGGAG(10/8)
Bsp1407I 602 TGTACA T^GTACA
BsrFI 1521 GCCGGC [GA]^CCGG[CT]
BsrGI 602 TGTACA T^GTACA
BstBI 48 TTCGAA TT^CGAA
BstUI 1051 CGCG CG^CG
Bsu36I 2938 CCTAAGG CC^T[ACGT]AGG
CelII 1197 GCTTAGC GC^T[ACGT]AGC
CfoI 1050 GCGC GCG^C
Cfr10I 1521 GCCGGC [GA]^CCGG[CT]
Eco81I 2938 CCTAAGG CC^T[ACGT]AGG
EcoNI 2938 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1516 CCTTGG C^C[AT][AT]GG
EcoT22I 490 ATGCAT ATGCA^T
HapII 1522 CCGG C^CGG
HhaI 1050 GCGC GCG^C
Hin1I 1923 GACGTC G[GA]^CG[CT]C
HinP1I 1050 GCGC G^CGC
HindIII 3505 AAGCTT A^AGCTT
HpaII 1522 CCGG C^CGG
KpnI 1859 GGTACC GGTAC^C
MamI 882 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1203 CAGCTG C[AC]G^C[GT]G
MspI 1522 CCGG C^CGG
MvnI 1051 CGCG CG^CG
NaeI 1521 GCCGGC GCC^GGC
NdeI 640 CATATG CA^TATG
NgoMIV 1521 GCCGGC G^CCGGC
NheI 155 GCTAGC G^CTAGC
NsiI 490 ATGCAT ATGCA^T
NspV 48 TTCGAA TT^CGAA
PflMI 876 CCAATAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 718 GTTTAAAC GTTT^AAAC
PshBI 1816 ATTAAT AT^TAAT
PvuII 1203 CAGCTG CAG^CTG
SapI 404 GAAGAGC GCTCTTC(1/4)
SfuI 48 TTCGAA TT^CGAA
SspBI 602 TGTACA T^GTACA
StuI 1592 AGGCCT AGG^CCT
StyI 1516 CCTTGG C^C[AT][AT]GG
Van91I 876 CCAATAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 3530 CCGC CCGC(-3/-1)
18 GCGG
AflII 3943 CTTAAG C^TTAAG
4162 CTTAAG
BalI 2388 TGGCCA TGG^CCA
2458 TGGCCA
BbsI 1421 GTCTTC GAAGAC(2/6)
1563 GTCTTC
BfrI 3943 CTTAAG C^TTAAG
4162 CTTAAG
BpuAI 1421 GTCTTC GAAGAC(2/6)
1563 GTCTTC
BsaI 1493 GAGACC GGTCTC(1/5)
823 GGTCTC
BsaJI 1192 CCAGGG C^C[ACGT][ACGT]GG
1516 CCTTGG
BsgI 2609 CTGCAC GTGCAG(16/14)
3677 CTGCAC
BsmFI 580 GGGAC GGGAC(10/14)
1512 GGGAC
BspDI 73 ATCGAT AT^CGAT
282 ATCGAT
BspLU11I 2963 ACATGT A^CATGT
3887 ACATGT
BssKI 1192 CCAGG ^CC[ACGT]GG
1805 CCTGG
BstAPI 679 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2853 GCATTTTTTGC
BstNI 1192 CCAGG CC^[AT]GG
1805 CCTGG
ClaI 73 ATCGAT AT^CGAT
282 ATCGAT
DraII 1512 GGGACCT [GA]G^G[ACGT]CC[CT]
3862 AGGTCCT
EcoO109I 1512 GGGACCT [GA]G^G[ACGT]CC[CT]
3862 AGGTCCT
EcoRI 226 GAATTC G^AATTC
4252 GAATTC
EcoRII 1192 CCAGG ^CC[AT]GG
1805 CCTGG
HincII 346 GTTGAC GT[CT]^[GA]AC
3332 GTCAAC
HindII 346 GTTGAC GT[CT]^[GA]AC
3332 GTCAAC
MluNI 2388 TGGCCA TGG^CCA
2458 TGGCCA
MscI 2388 TGGCCA TGG^CCA
2458 TGGCCA
MvaI 1192 CCAGG CC^[AT]GG
1805 CCTGG
PpuMI 1512 GGGACCT [GA]G^G[AT]CC[CT]
3862 AGGTCCT
PstI 150 CTGCAG CTGCA^G
2675 CTGCAG
ScrFI 1192 CCAGG CC^[ACGT]GG
1805 CCTGG
XbaI 694 TCTAGA T^CTAGA
3238 TCTAGA
XcmI 1499 CCAGATCGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3838 CCACTGTTAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbvI BclI BfaI BmyI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspHI
BsrDI BsrI Bst1107I BstF5I BstYI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI Sau3AI Sau96I
ScaI SfaNI SfcI SmlI SspI
SwaI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI