##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI AspEI AviII AvrII
BbvCI BciVI BclI BfrI BlnI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI Ksp632I KspI MamI MfeI
MluI MroI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI TaqI TfiI TthHB8I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1132 GGTACC G^GTACC
AccII 1396 CGCG CG^CG
Alw44I 305 GTGCAC G^TGCAC
ApaI 481 GGGCCC GGGCC^C
ApaLI 305 GTGCAC G^TGCAC
Asp700I 1549 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1132 GGTACC G^GTACC
AspI 2527 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2535 CCCGGG C^[CT]CG[GA]G
BamHI 2068 GGATCC G^GATCC
BbeI 153 GGCGCC GGCGC^C
BbrPI 1204 CACGTG CAC^GTG
BglI 156 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1451 AGATCT A^GATCT
BseRI 218 CTCCTC GAGGAG(10/8)
BsgI 822 GTGCAG GTGCAG(16/14)
BsiEI 2321 CGGTCG CG[GA][CT]^CG
BsoBI 2535 CCCGGG C^[CT]CG[GA]G
BspMI 301 GCAGGT ACCTGC(4/8)
BsrFI 670 ACCGGC [GA]^CCGG[CT]
BssHII 1395 GCGCGC G^CGCGC
BstAPI 1991 GCAAACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1396 CGCG CG^CG
BstXI 1909 CCAAATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 670 ACCGGC [GA]^CCGG[CT]
HaeII 153 GGCGCC [GA]GCGC^[CT]
HincII 2107 GTCAAC GT[CT]^[GA]AC
HindII 2107 GTCAAC GT[CT]^[GA]AC
HindIII 2576 AAGCTT A^AGCTT
HinfI 807 GACTC G^A[ACGT]TC
KasI 153 GGCGCC G^GCGCC
KpnI 1132 GGTACC GGTAC^C
MvnI 1396 CGCG CG^CG
NarI 153 GGCGCC GG^CGCC
PflFI 2527 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 807 GACTC GAGTC(4/5)
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
SacI 828 GAGCTC GAGCT^C
SfoI 153 GGCGCC GGC^GCC
SmaI 2535 CCCGGG CCC^GGG
SpeI 954 ACTAGT A^CTAGT
Tth111I 2527 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1458 TCTAGA T^CTAGA
XmaCI 2535 CCCGGG C^CCGGG
XmaI 2535 CCCGGG C^CCGGG
XmnI 1549 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1026 GTCTAC GT^[AC][GT]AC
2254 GTCTAC
AlwNI 966 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2171 CAGGGGCTG
BbsI 898 GTCTTC GAAGAC(2/6)
1150 GTCTTC
BcgI 707 CGACCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
346 GCATCTGCATCG
BlpI 2425 GCTTAGC GC^T[ACGT]AGC
2495 GCTTAGC
Bpu1102I 2425 GCTTAGC GC^T[ACGT]AGC
2495 GCTTAGC
BpuAI 898 GTCTTC GAAGAC(2/6)
1150 GTCTTC
BsaAI 30 TACGTG [CT]AC^GT[GA]
1204 CACGTG
BsmBI 473 CGTCTC CGTCTC(1/5)
592 GAGACG
BsrDI 1334 CATTGC GCAATG(2/0)
52 GCAATG
CelII 2425 GCTTAGC GC^T[ACGT]AGC
2495 GCTTAGC
DrdI 230 GACCCTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2055 GACTTGGAGGTC
FauI 801 GCGGG CCCGC(4/6)
1097 GCGGG
PstI 715 CTGCAG CTGCA^G
2383 CTGCAG
SexAI 321 ACCTGGT A^CC[AT]GGT
909 ACCAGGT
SphI 1513 GCATGC GCATG^C
2166 GCATGC
Tsp509I 1637 AATT ^AATT
1665 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AocI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI Tru9I TseI Tsp45I
TspRI Van91I XhoII